Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from <i>Arabidopsis thaliana</i>

Shen, Jinyu; Li, Zhiyong; Fu, Yajuan; Liang, Jiansheng

doi:10.7717/peerj.12673

Cited by 7 publications

(6 citation statements)

References 53 publications

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“…In particular, one isoform, CA1_ID11, was rapidly down-regulated under drought stress, suggesting that the CA1 protein produced by CA1_ID11 may be important for drought response. The CA1_ID11 isoform encodes a slightly shorter protein than the CA1.2 isoform (Figure 7C), and the protein produced by CA1_ID11 was mainly localized in the envelope, whereas the protein derived from CA1.2 appeared to be evenly distributed in the chloroplast stroma (Shen et al, 2021). The importance of CA1 localization was demonstrated by Hines et al (2021): Tobacco stromal CA1 (and CA5) isoforms play no role in photosynthesis but do play a role in plant development, whereas no such function could be ascribed to cytosolic CA1.…”

Section: Alternative Splicing Enhances Proteome Diversity To Countera...mentioning

confidence: 99%

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought

Qian

et al. 2023

Front. Plant Sci.

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Plants have evolved sophisticated mechanisms to cope with drought, which involve massive changes in nuclear gene expression. However, little is known about the roles of post-transcriptional processing of nuclear or organellar transcripts and how meaningful these changes are. To address these issues, we used RNA-sequencing after ribosomal RNA depletion to monitor (post)transcriptional changes during different times of drought exposure in Arabidopsis Col-0. Concerning the changes detected in the organellar transcriptomes, chloroplast transcript levels were globally reduced, editing efficiency dropped, but splicing was not affected. Mitochondrial transcripts were slightly elevated, while editing and splicing were unchanged. Conversely, alternative splicing (AS) affected nearly 1,500 genes (9% of expressed nuclear genes). Of these, 42% were regulated solely at the level of AS, representing transcripts that would have gone unnoticed in a microarray-based approach. Moreover, we identified 927 isoform switching events. We provide a table of the most interesting candidates, and as proof of principle, increased drought tolerance of the carbonic anhydrase ca1 and ca2 mutants is shown. In addition, altering the relative contributions of the spliced isoforms could increase drought resistance. For example, our data suggest that the accumulation of a nonfunctional FLM (FLOWERING LOCUS M) isoform and not the ratio of FLM-ß and -δ isoforms may be responsible for the phenotype of early flowering under long-day drought conditions. In sum, our data show that AS enhances proteome diversity to counteract drought stress and represent a valuable resource that will facilitate the development of new strategies to improve plant performance under drought.

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Section: Alternative Splicing Enhances Proteome Diversity To Countera...mentioning

confidence: 99%

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought

Qian

et al. 2023

Front. Plant Sci.

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“…We have studied the effect of the αCA4 gene knockout on the expression level of several genes encoding CAs located in the cytoplasm, chloroplast stroma, and thylakoid membrane. From four alternative splicing forms of βCA1 gene, there are two pairs with the same sequences on 3′ ends: βCA1.1 and βCA1.2 , and βCA1.3 and βCA1.4 [ 30 ]. Therefore, two forms of βCA1.1 and βCA1.2 , encoding the stromal βCA1, were determined together ( βCA1.1 + 1.2 ), as well as the other two, βCA1.3 and βCA1.4 ( βCA1.3 + 1.4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, two forms of βCA1.1 and βCA1.2 , encoding the stromal βCA1, were determined together ( βCA1.1 + 1.2 ), as well as the other two, βCA1.3 and βCA1.4 ( βCA1.3 + 1.4 ). Since the GFP fluorescence signal for βCA1.3, located in the chloroplast envelope, was much lower than that of βCA1.4, located in the cytoplasm [ 30 ], we consider βCA1.3+1.4 as transcripts encoding the cytoplasmic form of βCA1.…”

Section: Resultsmentioning

confidence: 99%

The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

Rudenko

Permyakova

Ignatova

et al. 2022

Plants

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An homozygous mutant line of Arabidopsis thaliana with a knocked out At4g20990 gene encoding thylakoid carbonic anhydrase αCA4 was created using a CRISPR/Cas9 genome editing system. The effects of the mutation were compared with those in two mutant lines obtained by the T-DNA insertion method. In αCA4 knockouts of all three lines, non-photochemical quenching of chlorophyll a fluorescence was lower than in the wild type (WT) plants due to a decrease in its energy-dependent component. The αCA4 knockout also affected the level of expression of the genes encoding all proteins of the PSII light harvesting antennae, the genes encoding cytoplasmic and thylakoid CAs and the genes induced by plant immune signals. The production level of starch synthesis during the light period, as well as the level of its utilization during the darkness, were significantly higher in these mutants than in WT plants. These data confirm that the previously observed differences between insertional mutants and WT plants were not the result of the negative effects of T-DNA insertion transgenesis but the results of αCA4 gene knockout. Overall, the data indicate the involvement of αCA4 in the photosynthetic reactions in the thylakoid membrane, in particular in processes associated with the protection of higher plants’ photosynthetic apparatus from photoinhibition.

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“…The rate constant of the catalyzed reaction is up to 10 6 times higher than that of the spontaneous reaction [1], with the hydration reaction being accelerated to a greater extent. Several CAs have been found in chloroplasts where photosynthetic reactions occur: α-CA1 [2] and two forms of the soluble CA, β-CA1 [3,4], are located in the stroma, while α-CA4 [5,6] and α-CA5 [7] are bound to the thylakoid membrane. A β-family CA was also discovered in the thylakoid lumen of pea and Arabidopsis [8,9].…”

Section: Introductionmentioning

confidence: 99%

Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2

Nadeeva

Ignatova

Rudenko

et al. 2023

Plants

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The knockout of the At2g28210 gene encoding α-carbonic anhydrase 2 (α-CA2) in Arabidopsis thaliana (Columbia) led to alterations in photosynthetic processes. The effective quantum yields of both photosystem II (PSII) and photosystem I (PSI) were higher in α-carbonic anhydrase 2 knockout plants (α-CA2-KO), and the reduction state of plastoquinone pool was lower than in wild type (WT). The electron transport rate in the isolated thylakoids measured with methyl viologen was higher in α-CA2-KO plants. The amounts of reaction centers of PSII and PSI were similar in WT and α-CA2-KO plants. The non-photochemical quenching of chlorophyll a fluorescence in α-CA2-KO leaves was lower at the beginning of illumination, but became slightly higher than in WT leaves when the steady state was achieved. The degree of state transitions in the leaves was lower in α-CA2-KO than in WT plants. Measurements of the electrochromic carotenoid absorbance shift (ECS) revealed that the light-dependent pH gradient (ΔpH) across the thylakoid membrane was lower in the leaves of α-CA2-KO plants than in WT plants. The starch content in α-CA2-KO leaves was lower than in WT plants. The expression levels of the genes encoding chloroplast CAs in α-CA2-KO changed noticeably, whereas the expression levels of genes of cytoplasmic CAs remained almost the same. It is proposed that α-CA2 may be situated in the chloroplasts.

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Identification and molecular characterization of the alternative spliced variants of beta carbonic anhydrase 1 (βCA1) from Arabidopsis thaliana

Cited by 7 publications

References 53 publications

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought

Response of the organellar and nuclear (post)transcriptomes of Arabidopsis to drought

The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

Features of Photosynthesis in Arabidopsis thaliana Plants with Knocked Out Gene of Alpha Carbonic Anhydrase 2

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