Functional Oxytocin Receptors in Bovine Granulosa Cells1

Okuda, Kiyoshi; Uenoyama, Yoshihisa; Fujita, Yuka; Iga, Kosuke; Sakamoto, Kazuichi; Kimura, Tadashi

doi:10.1095/biolreprod56.3.625

Cited by 49 publications

(32 citation statements)

References 25 publications

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“…Functional OT receptors have been detected in bovine granulosa cells, suggesting that OT may be an autocrine factor during follicular growth (Okuda et al 1997). OT also increased the rate of mouse blastocyst development and might therefore play some part in the early stage of development of fertilized oocytes (Furuya et al 1995).…”

Section: Expression Of Ctr Mrnamentioning

confidence: 99%

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Kanda

Takuwa‐Kuroda²,

Ukena³

et al. 2003

Journal of Endocrinology

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We reported that the common octopus, Octopus vulgaris, in common with vertebrates, possesses two members of the oxytocin/vasopressin superfamily: octopressin (OP) and cephalotocin (CT). This was the first observation of its kind in invertebrates. As OP and CT have different biological activities, the presence of specific receptors has been proposed. We cloned the cDNA of an orphan receptor from Octopus brain and found it to encode a polypeptide of 397 amino acids that displays sequences characteristic of G-protein coupled receptors. The orphan receptor showed high homology to receptors of the oxytocin/vasopressin superfamily and seemed to conserve the agonist-binding pocket common to the oxytocin and vasopressin receptors. Xenopus oocytes that express the orphan receptor responded to the application of CT by an induction of membrane Cl currents coupled to the inositol phosphate/Ca 2+ pathway. OP and the other members of the oxytocin/vasopressin superfamily did not activate this receptor. HPLC fractionation of the Octopus brain extract combined with an oocyte assay yielded a single substance that was identical to CT. On the basis of these results, we conclude that the cloned receptor is the CT receptor (CTR). Expression of CTR mRNA in Octopus was detected in the central and the peripheral nervous systems, the pancreas, the oviduct and the ovary. This receptor may mediate physiological functions of CT in Octopus such as neurotransmission, reproduction and metabolism.

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Section: Expression Of Ctr Mrnamentioning

confidence: 99%

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Kanda

Takuwa‐Kuroda²,

Ukena³

et al. 2003

Journal of Endocrinology

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“…Concentrations of progesterone were determined directly in the media from the cell culture with an enzyme immunoassay (EIA) as previously described [15]. The standard curve ranged from 0.39 to 100 ng/ml, and the effective dose for 50% inhibition (ED50) of the assay was 4.5 ng/ml.…”

Section: Progesterone Determinationmentioning

confidence: 99%

Progesterone Stimulation by Prostaglandin F2.ALPHA. Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells.

et al. 1998

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Abstract. The intracellular mechanism that mediates the stimulatory effect of prostaglandin F2α (PGF2α) on progesterone secretion by bovine luteal cells is not well understood. In the present study, we investigated to test the hypothesis that PGF2α stimulates progesterone secretion through activation of protein kinase C (PKC) by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to varying concentrations of PGF2α (10 -8 , 10 -7 , 10 -6 M) or luteinizing hormone (LH; 100 ng/ml) for 4, 6, or 16 h. Treatment of the cells with the highest dose of PGF2α (10 -6 M) or LH resulted in increases in progesterone secretion for all three exposure times. Following down-regulation of PKC with a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA; 10 -6 M), the stimulatory effect of PGF2α was no longer evident, whereas the response to LH was unaffected.These results support the hypothesis that the luteotropic action of PGF2α is mediated by the activation of intracellular PKC.

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“…Progesterone concentrations in the cell culture medium were determined directly by an enzyme immunoassay, as described previously (Okuda et al 1997). The significance of values was analyzed by Duncan's new multiple range test.…”

Section: Isolation Of Granulosa Cells and Measurement Of Progesteronementioning

confidence: 99%

Expression and purification of biologically active porcine follicle-stimulating hormone in insect cells bearing a baculovirus vector

Kato

Sato

Ihara

et al. 1998

Journal of Molecular Endocrinology

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Biologically active recombinant porcine FSH (rec-pFSH) free from the cognate pituitary glycoprotein hormone LH was produced. It was synthesized by a baculovirus vector-insect cell system using two cDNAs encoding the glycoprotein and FSH subunits. Its antigenicity was the same as that of pFSH prepared from the pituitary. Glycosylation of rec-pFSH was shown by tunicamycin treatment but the molecular mass of each subunit was lower than that of pituitary-derived FSH, because of the absence of trimming of terminal sugars in insect cells. Rec-pFSH was secreted into the culture medium at about 1 mg/l and purified in six fractions, because of the heterogeneity of the sugar group, by S-Sepharose and concanavalin A-Sepharose column chromatography. The biological activity of rec-pFSH was examined by measuring its effect on progesterone secretion from porcine granulosa cells and germinal vesicle breakdown (GVBD) of porcine oocytes. It showed adequate activity with respect to progesterone secretion, although some fractions rich in the sugar group showed lower activity than that of pituitary-derived FSH. It exhibited higher GVBD activity than that of pituitary-derived FSH at concentrations as low as 1 ng/ml. These results demonstrate that the baculovirus vector-insect cell system can provide biologically active rec-pFSH.

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Functional Oxytocin Receptors in Bovine Granulosa Cells1

Cited by 49 publications

References 25 publications

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Progesterone Stimulation by Prostaglandin F2.ALPHA. Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells.

Expression and purification of biologically active porcine follicle-stimulating hormone in insect cells bearing a baculovirus vector

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