Progesterone Stimulation by Prostaglandin F2.ALPHA. Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells.

Okuda, Kiyoshi; Uenoyama, Yoshihisa; Lee, Kang Woo; Sakumoto, Ryosuke; Jan, Skarzynski Dariusz

doi:10.1262/jrd.44.79

Cited by 56 publications

(78 citation statements)

References 31 publications

(41 reference statements)

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“…Thus, the dominant intracellular mechanisms involved in the action of PGF2α is the activation of the PKC cascade in bovine luteal cells. Indeed, recent data from bovine CL cells in culture showed a lack of P stimulation in PKC-deficient cells [8]. The present in vivo data are consistent with the above findings.…”

Section: Discussionsupporting

confidence: 93%

“…In these in vitro studies, PGF2α clearly activated intracellular mechanisms involved in the cascade of stimulation of protein kinase C (PKC) [3][4][5] and free calcium ion (Ca 2+ ) [4][5][6][7]. Recently, it has been further demonstrated that PGF2α failed to stimulate the release of P from PKC-deficient bovine luteal cells, suggesting that the luteotropic action of PGF2α is mediated by the activation of intracellular PKC in culture [8]. These results support the hypothesis that luteal PGF2α may have a dual function (luteotropic in young CL, but luteolytic in aged CL).…”

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confidence: 99%

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Direct Effect of PGF2.ALPHA., TPA and lonophore A23187 on Progesterone Release from Microdialyzed Corpus Luteum inthe Cow.

Ohtani

Kobayashi

Miyamoto

1999

Journal of Reproduction and Development

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Abstract. There is general agreement that prostaglandin F2α (PGF2α) is a physiological luteolysin in the cow. Contrary to this fact, PGF2α has been shown to stimulate the progesterone (P) release from luteal cells in vitro, through the activation of intracellular protein kinase C (PKC) and free calcium ion (Ca 2+ ). To examine the possibility that an essential factor or function(s) may be lost through the process of preparing cells from the corpus luteum (CL), we evaluated the direct effect of PGF2α, TPA and ionophore A23187 on local P release within the CL in the cow, by utilizing in vivo microdialysis system (MDS). Normal cyclic Holstein cows (n=4) were superovulated with FSH and the PGF2α analogue, and on Day 8 after estrus, they were surgically implanted with the MDS (cutoff Mr=1000 kDa) into multiple CL (1-3 line/CL). The system was continuously perfused with Ringer's solution from immediately after the surgery. On Days 10-11, several CL were assigned to the control, PGF2α (10 -5 M), TPA (10 -5 M), A23187 (10 -5 M), PGF2α+A23187 and TPA+A23187. The stimulants were infused into the CL using the MDS for 6 h, and each line of the MDS was further perfused with Ringer's solution for 26 h. A 6-h infusion with PGF2α stimulated P release during infusion, but after infusion the P release was again stable around the baseline. A 6-h infusion with TPA or A23187 strongly stimulated the P release. Similarly, PGF2α+A23187 or TPA+A23187 stimulated the P release. During the last 8 h of the experiment, A23187 alone, PGF2α+A23187 or TPA+A23187 inhibited the P release, whereas TPA as well as PGF2α maintained the same level as in the control. The results indicate that the direct exposure of the microenvironment in the bovine CL to PGF2α in vivo does not result in the suppression of P release until 26 h after stimulation, and that the activation of cytosolic free calcium is more effective in inhibiting the P release than the stimulation of PKC during the following period. Thus, the results support the concept that PGF2α needs some local luteolytic intermediator(s) that may enhance the intracellular calcium concentration.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Direct Effect of PGF2.ALPHA., TPA and lonophore A23187 on Progesterone Release from Microdialyzed Corpus Luteum inthe Cow.

Ohtani

Kobayashi

Miyamoto

1999

Journal of Reproduction and Development

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“…Based on an in vitro study (Speroff & Ramwell 1970), a direct PGF steroidogenic stimulation of luteal cells has been suggested (Hixon & Hansel 1974). Direct inhibitory and stimulating effects of PGF on small and large luteal cells respectively have been demonstrated in vitro (Alila et al 1988, Meidan et al 1992, Girsh et al 1995, Okuda et al 1998, Skarzynski & Okuda 1999. In addition, the results of studies involving treatment with a bolus luteolytic dose of PGF have been interpreted to indicate that nitric oxide is a crucial factor in initiating luteolysis through a drastic increase in luteal blood flow (Shirasuna et al 2008), and that the induced increase in luteal blood flow is the first step in the luteolytic cascade (Miyamoto et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Shrestha¹,

Beg²,

Imam³

et al. 2010

Reproduction

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Progesterone and luteal blood flow effects of an i.u. 2-h infusion of 0.25 mg/h of prostaglandin F 2a (PGF) that simulated a natural pulse of 13,14-dihydro-15-keto-PGF (PGFM) were compared to the effects of a single bolus i.u. injection of PGF (4 mg) that induced complete luteolysis in heifers. Blood sampling and an estimate of the percentage of luteal area with colour-Doppler signals of blood flow were performed every 2 min for 20 min and less frequently thereafter for 6 h. After the beginning of PGF infusion or a bolus injection, progesterone increased to a peak at 14 and 10 min respectively, and was accompanied by an increase in blood flow in the bolus group but not in the infusion group. Progesterone then decreased for 1 or 2 h and was accompanied by a continued elevation in blood flow in the PGF bolus group and by a slight increase in the PGF infusion group. Progesterone then rebounded in both groups, but the rebound was greater in the infusion group. Blood flow decreased during the descending arm of the progesterone rebound. Cortisol and prolactin began to increase 6 min after the bolus PGF injection but did not increase during or after PGF infusion. The increases in cortisol, prolactin and blood flow after a PGF bolus treatment but not during a simulated PGFM pulse indicated that the bolus treatment was pharmacologic, and its use may lead to faulty conclusions on the nature of physiologic luteolysis. The comparisons between progesterone and blood flow are novel.

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“…Ryan et al [10,11] indicated that a high fat diet causes increased frequency of luteal activity and alterations in specific aspects of ovarian steroidogenic potential. Other researchers have shown that PL activates protein kinase, which controls luteal cells [12,13], and that lipoprotein, which is composed of PL, regulates P4 production in luteal cells [14,15].…”

Section: Discussionmentioning

confidence: 99%

Relationships between the Conception Rate of Estrus Synchronization Using Estradiol Benzoate and CIDR (Progesterone) and Other Parameters in Holstein Lactating Dairy Cows

Miura

Kotani

Kohiruimaki

et al. 2008

Journal of Reproduction and Development

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Abstract. The purpose of this study was to determine the relationships between conception rate and other parameters before estrus synchronization with a Controlled Internal Drug Release Device (CIDR) and estradiol benzoate (EB). In the estrus synchronization program, animals were injected with 2 mg EB and then received a CIDR. Seven days later, the CIDR was removed and the animals were given an injection of Prostaglandin F2α. Twenty-four hours later, they received an injection of 1 mg EB, and they were artificially inseminated 24 h after that. This program was applied to 258 Holstein cows in Tohoku-machi (Aomori, Japan). Blood was collected at the beginning of the program, and the conception rate was determined about 40 days after insemination. The relationships among conception rate, blood biochemical values, age, body condition score and days in milk were statistically analyzed to determine better conditions for cow conception. The conception rate of the cows in the high progesterone group (more than 1 ng/ml, P4+) was significantly higher than that of the low progesterone group (less than 1 ng/ml, P4-; 47.9% vs. 28.6% P<0.01). In the P4-groups, the serum phospholipid level was significantly higher in the conception group than in the nonconception group, and the same tendency was seen in the P4+ groups. Blood urea nitrogen (BUN), albumin (Alb), and total cholesterol (TChol) were significantly higher in the conception group compared with the non-conception group, but no with P4 was observed. We concluded that 1) the conception rate of the P4-group was remarkably low, that 2) the low conception rate and low P4 level was related to a low PL level and that 3) BUN, Alb and TChol were higher in the conception group, although no relation with P4 was found. Key words: Conception rate, Controlled Internal Drug Release Device (CIDR), Estrus synchronization program, Holstein cow (J. Reprod. Dev. 54: [214][215][216] 2008) ecline of the conception rate and extension of the calving interval in the dairy cow resulted in economic losses. In recent years, decline of the conception rate has become a serious problem [1,2]. One problem related to this is low fertilization resulting from an unsuitable nutrition status after calving. Some researchers have shown that low energy intake or body condition score (BCS) causes decreased conception rates [3,4]. Milking requires expenditure of energy, and if the animal's BCS is low, increase of the negative energy balance (NEB) causes a negative influence on breeding function.Another problem is that the estrus signs become weak and AI timing is unsuitable [5]. To solve these problems, some estrus synchronization programs, such as ovsynch, heatsynch [6], quicksynch [7] and modified programs using a Controlled Internal Drug Release (CIDR) have been developed. In this study, a synchronizing method that utilizes EB and a CIDR was used to clarify the influence of nutrition and internal secretion state at the time of synchronization start. Materials and Methods AnimalsA total of 258 lactating H...

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Progesterone Stimulation by Prostaglandin F2.ALPHA. Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells.

Cited by 56 publications

References 31 publications

Direct Effect of PGF2.ALPHA., TPA and lonophore A23187 on Progesterone Release from Microdialyzed Corpus Luteum inthe Cow.

Direct Effect of PGF2.ALPHA., TPA and lonophore A23187 on Progesterone Release from Microdialyzed Corpus Luteum inthe Cow.

Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Relationships between the Conception Rate of Estrus Synchronization Using Estradiol Benzoate and CIDR (Progesterone) and Other Parameters in Holstein Lactating Dairy Cows

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