Functional monitoring of lung tissue using a hybrid hyperspectral Time-Resolved GASMAS system: a systematic study on ex vivo sample.

Lacerenza, Michele; Pacheco, Andrea; Sekar, Sanathana Konugolu Venkata; Nogueira, Marcelo Saito; Buttafava, Mauro; Tosi, Alberto; Pifferi, Antonio; Contini, Davide; Andersson‐Engels, Stefan

doi:10.1364/ots.2020.sw1d.2

Cited by 10 publications

(4 citation statements)

References 6 publications

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“…They include diffuse reflectance spectroscopy 7,8 , endogenous 9 or exogenous fluorescence spectroscopy 10,11 , optical coherent tomography 12 , and Raman spectroscopy 13 . Much effort is also being devoted to model the photon propagation through the lung by means of simulations 14,15 , anthropomorphic lung phantoms 16,17 , and ex vivo bovine lung tissues 18 . Conversely, there have not been many attempts to probe the lung non-invasively by optical transcutaneous means.…”

Section: Introductionmentioning

confidence: 99%

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

Pifferi,

Miniati,

Farina

et al. 2023

Preprint

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Non-invasive in vivo sensing of the lung with light would help diagnose and monitor pulmonary disorders (caused by e.g., emphysema, immature lung tissue in infants, Covid-19). We investigated the possibility to probe the lung with time domain diffuse optics, taking advantage of the increased depth (few cm) reached by photons detected after a long (few ns) propagation time. An initial study on 5 healthy volunteers included time-resolved broadband diffuse optical spectroscopy measurements at 3 cm source-detector distance over the 600-1100 nm range, and long-distance (7-9 cm) measurements at 820 nm performed during a breathing protocol. The interpretation of the in vivo data with a simplified homogeneous model yielded a maximum probing depth of 2.6-3.9 cm, suitable to reach the lung. Also, signal changes related to the inspiration act were observed, especially at long photon propagation times. Yet, intra- and inter-subject variability and inconsistencies, possibly alluring to competing scattering and absorption effects, prevented a straightforward interpretation. Aspects to be further investigated to gain a deeper insight are discussed.

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Section: Introductionmentioning

confidence: 99%

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

Pifferi,

Miniati,

Farina

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Therefore, scattering is associated with the tissue microstructure. On the other hand, absorption is associated with the tissue biomolecular composition, as its absorption bands are dependent on the molecular energy levels (including electronic [ 10 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], vibrational [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ] and rotational levels [ 57 ]). Tissue absorption is related to biological chromophores (or absorbing biomolecules), which were previously investigated in a number of clinical and preclinical studies [ 58 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

Nogueira

Maryam

Amissah

et al. 2022

Cancers

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Colorectal cancer (CRC) is the third most common and second most deadly type of cancer worldwide. Early detection not only reduces mortality but also improves patient prognosis by allowing the use of minimally invasive techniques to remove cancer while avoiding major surgery. Expanding the use of microsurgical techniques requires accurate diagnosis and delineation of the tumor margins in order to allow complete excision of cancer. We have used diffuse reflectance spectroscopy (DRS) to identify the main optical CRC biomarkers and to optimize parameters for the integration of such technologies into medical devices. A total number of 2889 diffuse reflectance spectra were collected in ex vivo specimens from 47 patients. Short source-detector distance (SDD) and long-SDD fiber-optic probes were employed to measure tissue layers from 0.5 to 1 mm and from 0.5 to 1.9 mm deep, respectively. The most important biomolecules contributing to differentiating DRS between tissue types were oxy- and deoxy-hemoglobin (Hb and HbO2), followed by water and lipid. Accurate tissue classification and potential DRS device miniaturization using Hb, HbO2, lipid and water data were achieved particularly well within the wavelength ranges 350–590 nm and 600–1230 nm for the short-SDD probe, and 380–400 nm, 420–610 nm, and 650–950 nm for the long-SDD probe.

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“…They include diffuse reflectance spectroscopy [7,8], endogenous [9] or exogenous fluorescence spectroscopy [10,11], optical coherent tomography [12], and Raman spectroscopy [13]. Much effort is also being devoted to model the photon propagation through the lung by means of simulations [14,15] anthropomorphic lung phantoms [16,17], and ex vivo bovine lung tissues [18]. Conversely, there are not many attempts to probe the lung optically non-invasively transcutaneously.…”

Section: Introductionmentioning

confidence: 99%

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

Pifferi¹,

Miniati²,

Farina³

et al. 2022

Preprint

View full text Add to dashboard Cite

Non-invasive in vivo sensing of the lung with light would help diagnose and monitor pulmonary disorders (caused by e.g. COVID-19, emphysema, immature lung tissue in infants). We investigated the possibility to probe the lung with time domain diffuse optics, taking advantage of the increased depth (few cm) reached by photons detected after a long (few ns) propagation time. An initial study on 5 healthy volunteers included time-resolved broadband diffuse optical spectroscopy measurements at 3 cm source-detector distance over the 600-1100 nm range, and long-distance (6-9 cm) measurements at 820 nm performed during a breathing protocol. The interpretation of the in vivo data with a simplified homogeneous model yielded a maximum probing depth of 2.6-3.9 cm, suitable to reach the lung. Also, signal changes related to the inspiration act were observed, especially at high photon propagation times. Yet, intra-and inter-subject variability and inconsistencies, possibly alluring to competing scattering and absorption effects, prevented a simple interpretation. Aspects to be further investigated to gain a deeper insight are discussed.

show abstract

Functional monitoring of lung tissue using a hybrid hyperspectral Time-Resolved GASMAS system: a systematic study on ex vivo sample.

Cited by 10 publications

References 6 publications

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

Initial non-invasive in vivo sensing of the lung using time domain diffuse optics

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