Functional Melatonin Receptors in Rat Ovaries at Various Stages of the Estrous Cycle

Soares, José Maria; Masana, Monica I.; Erşahin, Çağatay; Dubocovich, Margarita L.

doi:10.1124/jpet.103.049916

Cited by 175 publications

(139 citation statements)

References 40 publications

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“…This is consistent with the earlier reports on human and rat demonstrating the presence of MTNR1A and MTNR1B in granulosa cells and luteal cells (Soares et al 2003). The presence of high levels of melatonin in follicular fluid in human and binding sites in the ovary (Brzezinski et al 1987, Ronnberg et al 1990, Niles et al 1999 and, now, the presence of MTNR1A and MTNR1B in ovary suggest that melatonin directly acts as a modulator of ovarian functions.…”

Section: Discussionsupporting

confidence: 82%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

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The aim of the present study was to evaluate the seasonal variation in serum melatonin levels and their relationship to the changes in the serum progesterone level, ovarian steroidogenesis, and embryonic development during two successive pregnancies of Cynopterus sphinx. Circulating melatonin concentrations showed two peaks; one coincided with the period of low progesterone synthesis and delayed embryonic development, whereas the second peak coincided with regressing corpus luteum. This finding suggests that increased serum melatonin level during November-December may be responsible for delayed embryonic development by suppressing progesterone synthesis. The study showed increased melatonin receptors (MTNR1A and MTNR1B) in the corpus luteum and in the utero-embryonic unit during the period of delayed embryonic development. The in vitro study showed that a high dose of melatonin suppressed progesterone synthesis, whereas a lower dose of melatonin increased progesterone synthesis by the ovary. The effects of melatonin on ovarian steroidogenesis are mediated through changes in the expression of peripheral-type benzodiazepine receptor, P450 side chain cleavage enzyme, and LH receptor proteins. This study further showed a suppressive impact of melatonin on the progesterone receptor (PGR) in the utero-embryonic unit; this effect might contribute to delayed embryonic development in C. sphinx. The results of the present study thus suggest that a high circulating melatonin level has a dual contribution in retarding embryonic development in C. sphinx by impairing progesterone synthesis as well as by inhibiting progesterone action by reducing expression of PGR in the utero-embryonic unit. Reproduction (2009) 138 935-944

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Section: Discussionsupporting

confidence: 82%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

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“…Reported increases in prolificacy subsequent to the administration of melatonin in sheep might be due to the direct effect of exogenous melatonin on the ovine oocyte, which has been observed in other species (Ishizuka et al, 2000;Na et al, 2005;Parka et al, 2006). Several studies have reported the presence of melatonin in human pre-ovulatory follicular fluid and the seasonal variation of its contents (Brzezinski et al, 1987;Yie et al, 1995), and melatonin receptors have been found on rat, mouse, and porcine ovarian cells (Soares et al, 2003;Na et al, 2005;Kang et al, 2008), which suggests that melatonin has a direct effect on oocyte competence.…”

mentioning

confidence: 99%

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Casao

Abecia

Pérez

et al. 2010

Span. j. agric. res.

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The aim of this study was to assess the effects of melatonin on the in vitro maturation and fertilization of sheep oocytes, and on the in vitro culture of the embryos. Oocytes from sheep ovaries collected at the slaughterhouse were divided into four groups, two of which were treated with either 10E5 M (M5) or 10E6 M (M6) melatonin, while the other two groups served as untreated controls (C5 and C6). After in vitro fertilization with fresh ram semen, the embryos produced in each group were divided into two sets, one cultured with melatonin (M5M, C5M, M6M and C6M), and the other without melatonin (M5C, C5C, M6C, and C6C). A melatonin concentration of 10E6 M increased maturation rate (82.5% vs. 73.7% for M6 and C6, respectively; P < 0.05) and tended to improve cleavage rate 36 hours after in vitro fertilization (79.4% vs. 72.6% for M6 and C6, respectively, P = 0.08). A higher melatonin concentration (10E5 M) did not have significant effects on those parameters. Blastocyst rates on day 8 did not differ significantly among groups.

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“…Melatonin is found in ovarian follicular fluid (Rönnberg et al, 1990), suggesting a direct effect of this hormone on ovarian function. The effects of melatonin on ovarian function vary with tissue structure, cell type, and with the fact whether the species is a seasonal or non-seasonal breeder (Soares et al, 2003). Two high-affinity melatonin receptor types, MTNR1A and MTNR1B, have been cloned in humans, sheep, Siberian hamsters, mice, and rats (Nishiyama et al, 2009) and found to exhibit different molecular structures and chromosomal locations among these species.…”

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confidence: 99%

Genetic effects of melatonin receptor genes on chicken reproductive traits

Li¹,

Zhang²,

Smith³

et al. 2013

Czech J. Anim. Sci.

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ABSTRACT:The melatonin receptors are G protein-coupled receptors (GPCR) that bind melatonin. Three types of melatonin receptors have been cloned. The MTNR1A (or Mel1A or MT1) and MTNR1B (or Mel1B or MT2) receptor subtypes are present in humans and other mammals, while an additional melatonin receptor subtype MTNR1C (or Mel1C or MT3) has been identified in amphibians and birds. Previous research has shown that the three common melatonin receptors regulate physiological processes, including seasonal reproduction and ovarian physiology. However, whether or not any polymorphisms of the different melatonin receptor subtypes are associated with reproductive traits in chickens is not known. In this study, we performed candidate gene analysis to identify single-nucleotide polymorphisms (SNPs) in the MTNR1A, MTNR1B, and MTNR1C genes in the Erlang Mountain Chicken population. SNP discovery was achieved by sequencing pooled DNA samples. Direct PCR-sequencing, PCR-SSCP/PCR-sequencing, and PCR-RFLP method were used to genotype the MTNR1A, MTNR1B, and MTNR1C genes, respectively. The GLM Procedure was used to estimate the statistical significance of association between genotypes at each locus and reproductive traits of chickens. In a sample of 460 chickens, four novel polymorphisms (JQ249890:g.384T>C, JQ249891:g.387T>C, JQ249894:g.63C>T, and JQ249896:g.294G>A) were detected in the melatonin receptor genes MTNR1A, MTNR1B, and MTNR1C, respectively. A statistically significant association (P < 0.01) was found between two SNPs (MTNR1A SNP, MTNR1C SNP) and reproductive traits: egg number at 300 days of age (EN) and age at first egg (AFE).

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Functional Melatonin Receptors in Rat Ovaries at Various Stages of the Estrous Cycle

Cited by 175 publications

References 40 publications

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Genetic effects of melatonin receptor genes on chicken reproductive traits

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