The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Casao, A.; Abecia, J. A.; Pérez, J.A. Cebrián; Blanco, Teresa; Vázquez, M.I.; Forcada, F.

doi:10.5424/sjar/2010081-1141

Cited by 36 publications

(21 citation statements)

References 38 publications

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“…Contrary to our previous results (Soto‐Heras et al, ) we did not find a higher blastocyst yield with melatonin. Other studies neither found improvement on blastocyst production (Casao et al, ; Rodrigues‐Cunha et al, ; Takada, Martins Junior, Mingoti, Balieiro, & Coelho, ) which denotes a variability in results depending on specific culture conditions. However, we observed an increase on the ICM number and ICM:TE ratio in hatched blastocysts.…”

Section: Discussionmentioning

confidence: 92%

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Soto-Heras

Catalá

Roura

et al. 2018

Reprod Domestic Animals

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Contents Melatonin enhances in vitro embryo development in several species by improving the oocyte developmental competence during in vitro maturation (IVM). Melatonin has a wide range of actions, from scavenging reactive oxygen species (ROS) to regulating gene expression, and it can also act by way of melatonin receptors. The aim of this study was to determine the mechanism of action of melatonin during the IVM of juvenile goat oocytes and the role of the membrane receptors. Melatonin receptor 1 was immunolocalized in cumulus cells and oocytes before and after 24 hr of IVM. The effect of melatonin on oocyte developmental competence was tested in three experimental IVM groups: (a) control, (b) 10−7 M melatonin, and (c) 10−7 M melatonin +10−7 M luzindole (an inhibitor of both melatonin receptors). After IVM oocytes were assessed for ROS levels, mitochondrial activity, adenosine 5′‐triphosphate (ATP) concentration and relative gene expression (ACTB, SLC1A1, SOD1, GPx1, BAX, DNMT1, GCLC and GDF9). IVM‐oocytes were in vitro fertilized and cultured under conventional conditions. Blastocyst rate and quality (differential cell count) were assessed at 8 days post‐fertilization. Melatonin decreased ROS levels, increased mitochondrial activity and ATP content and increased blastocyst quality compared to control group (55.8 vs. 30.4 inner cell mass ICM, p < 0.05). There was no effect on the relative gene expression due to treatment with melatonin. In conclusion, we have showed that melatonin improves oocyte developmental competence in juvenile goats by reducing ROS levels and improving mitochondrial activity.

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Section: Discussionmentioning

confidence: 92%

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Soto-Heras

Catalá

Roura

et al. 2018

Reprod Domestic Animals

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“…For example, when melatonin was applied in the pig oocyte maturation medium, the rate of in vitro maturation of oocyte, parthenogenetic blastocyst embryos, and blastocyst cell number were significantly increased; these were accompanied by a substantial reduction in ROS in the melatonin‐treated embryos . It is reported that 10 −6 m melatonin also promoted the maturation rate and cleavage rate in sheep after in vitro fertilization . In addition to studies regarding melatonin's effects on oocytes, Pang et al.…”

Section: Introductionmentioning

confidence: 99%

Melatonin promotes the in vitro development of pronuclear embryos and increases the efficiency of blastocyst implantation in murine

Wang

Tian

Zhang

et al. 2013

Journal of Pineal Research

147

103

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When a defect occurs in the in vitro development of a pronuclear embryo, the interruption of the subsequent implantation limits the success of assisted conception. This common problem remains to be solved. In this study, we observed that melatonin at its physiological concentration (10(-7) m) significantly promoted the in vitro development of murine pronuclear embryos. This was indicated by the increased blastocyst rate, hatching blastocyst rate, and blastocyst cell number with melatonin treatment. In addition, when these blastocysts were implanted into female recipient mice, the pregnancy rates (95.0% versus control 67.8%), litter sizes (4.1 pups/litter versus control 2.7 pups/litter), and postnatal survival rates of offspring (96.84% versus control 81.24%) were significantly improved compared with their non-melatonin-treated counterparts. Mechanistic studies revealed that melatonin treatment upregulates gene expression of the antioxidant enzyme, superoxide dismutase (SOD), and the anti-apoptotic factor bcl-2 while downregulating the expression of pro-apoptotic genes p53 and caspase-3. Due to these changes, melatonin treatment reduces ROS production and cellular apoptosis during in vitro embryo development and improves the quality of blastocysts. The implantation of blastocysts with higher quality leads to more healthy offspring and increased pup survival.

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“…antitumour activity (Zou et al., ), antioxidant protection (Ginther et al., ), enhances immune function (Srinivasan et al., ), sleep (Dawson & Encel, ) and reproduction (Reiter et al., )). Additionally, the melatonin as an antioxidant and free radical scavenger reduces the oxidative damage and dysfunction of oocytes during maturation (Casao et al., ; Tian et al., ) and embryo culture (Wang et al., , ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, oxidative stress detection with direct or indirect measures such as ROS production, amount of intracellular GSH, DNA damage or gene expression could be used to develop and evaluate strategies to prevent or minimize oxidative dysfunction during embryonic development (Kwak et al., ; Mukherjee et al., ). Furthermore, the inclusion of antioxidants or free radical scavengers to the maturation (Casao et al., ; Tian et al., ) and culture medium (Wang et al., , ) may reduce oxidative stress and improve conditions for embryonic development.…”

Section: Introductionmentioning

confidence: 99%

Melatonin reduces apoptotic cells, SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts

Marques

Santos

Diesel

et al. 2017

Reprod Domestic Animals

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Effects of adding different concentrations of melatonin (10 , 10 and 10 M) to maturation (Experiment 1; Control, IVM + 10 , IVM + 10 , IVM + 10 ) and culture media (Experiment 2; Control, IVC + 10 , IVC + 10 , IVC + 10 ) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10 M) from Experiments 1-2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM + 10 , IVC + 10 , IVM/IVC + 10 ). In Experiment 1, maturated oocytes from Control and IVM + 10 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC + 10 (43.5%; 56.7%) and IVC + 10 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC + 10 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC + 10 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM/IVC + 10 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC + 10 treatment also had a higher mRNA expression of antioxidant gene (SOD2) compared to the Control, as well as the heat shock protein (HSPB1) compared to the IVM + 10 . Reactive oxygen species production was greater in the IVM/IVC + 10 treatment group. In conclusion, the 10 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.

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The effects of melatonin on in vitro oocyte competence and embryo development in sheep

Cited by 36 publications

References 38 publications

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Melatonin promotes the in vitro development of pronuclear embryos and increases the efficiency of blastocyst implantation in murine

Melatonin reduces apoptotic cells, SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts

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