Functional identification of a fatty acid Δ<sup>5</sup> desaturase gene from <i>Caenorhabditis elegans</i>

Michaelson, Louise V.; Napier, Johnathan A.; Lewis, Mervyn J.; Griffiths, Gareth; Lazarus, Colin M.; Stobart, A. K.

doi:10.1016/s0014-5793(98)01385-4

Cited by 81 publications

(53 citation statements)

References 27 publications

(37 reference statements)

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“…Full-length cDNAs for Δ6 desaturases have been isolated from the filamentous fungus Mortierella alpina (Huang et al, 1999), the nematode Caenorhabditis elegans (Napier et al, 1998), rat (Aki et al, 1999), mouse and human (Cho et al, 1999a). Fatty acid Δ5 desaturase genes have been isolated from M. alpina (Michaelson et al, 1998a), C. elegans (Michaelson et al, 1998b;Watts and Browse, 1999) and human (Cho et al, 1999b;Leonard et al, 2000). Using extant sequence information, we isolated a cDNA from the freshwater zebrafish (Danio rerio) with high similarity to mammalian Δ6 desaturase genes (Hastings et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Zheng

Seiliez

Hastings

et al. 2004

Comparative Biochemistry and Physiology Part B: Biochemistry an

172

139

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Abstract:Fish are the most important dietary source of the n-3 highly unsaturated fatty acids (HUFA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), that have particularly important roles in human nutrition reflecting their roles in critical physiological processes. The objective of the study described here was to clone, functionally characterise and compare expressed fatty acid desaturase genes involved in the production of EPA and DHA in freshwater and marine teleost fish species.Putative fatty acid desaturase cDNAs were isolated and cloned from common carp (Cyprinus carpio) and turbot (Psetta maximus). The enzymic activities of the products of these cDNAs, together with those of cDNAs previously cloned from rainbow trout (Oncorhynchus mykiss) and gilthead seabream (Sparus aurata), were determined by heterologous expression in the yeast containing the haem-binding motif, HPGG. Functional expression showed all four fish cDNAs encode basically unifunctional Δ6 fatty acid desaturase enzymes responsible for the first and ratelimiting step in the biosynthesis of HUFA from 18:3n-3 and 18:2n-6. All the fish desaturases were more active towards the n-3 substrate with 59.5%, 31.5%, 23.1% and 7.0% of 18:3n-3 being converted to 18:4n-3 in the case of turbot, trout, seabream and carp, respectively. The enzymes also showed very low, probably physiologically insignificant, levels of Δ5 desaturase activity, but none of the products showed Δ4 desaturase activity. The cloning and characterisation of desaturases from these fish is an important advance, as they are species in which there is a relative wealth of data on the nutritional regulation of fatty acid desaturation and HUFA synthesis, and between which substantive differences occur.

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Section: Introductionmentioning

confidence: 99%

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Zheng

Seiliez

Hastings

et al. 2004

Comparative Biochemistry and Physiology Part B: Biochemistry an

172

139

View full text Add to dashboard Cite

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“…Full length cDNAs for Δ6 fatty acid desaturases have been isolated from the nematode Caenorhabditis elegans , rat (Aki et al, 1999), mouse and human (Cho et al, 1999a). Fatty acid Δ5 desaturase genes have been isolated from C. elegans (Michaelson et al, 1998;Watts and Browse, 1999) and human (Cho et al, 1999b;Leonard et al, 2000a). A fatty acid desaturase has been cloned from zebrafish that has been shown to have both Δ6 and Δ5 desaturase activities (Hastings et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar)

et al. 2004

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Fish are an important dietary source of the long-chain C 20 and C 22 highly unsaturated fatty acids (HUFA), arachidonate (20:4n-6), eicosapentaenoate (20:5n-3) and docosahexaenoate (22:6n-3), that are crucial to the health of higher vertebrates and that can be beneficial in human diets. Δ5 and Δ6 fatty acid desaturases, and fatty acid elongases are critical enzymes in the biosynthetic pathways of HUFA from shorter chain C 18 polyunsaturated fatty acids (PUFA) such as linoleic (18:2n-6) and α-linolenic (18:3n-3) acids. Recently, full-length cDNAs for fatty acid desaturase and elongase enzymes have been cloned from Atlantic salmon. Functional characterisation of the desaturase revealed n-3 Δ5 desaturase activity, whereas the elongase had broad substrate specificity for PUFA with a range of chain lengths from C 18 to C 22 . The study described here was primarily focused on the nutritional regulation of genes involved in the HUFA biosynthetic pathway in Atlantic salmon. A feeding trial was performed whereby salmon smolts in seawater pens were fed for 40 weeks on five different diets. The diets consisted of a control diet containing fish oil (FO) and four diets in which the FO was replaced in a graded manner by linseed oil (LO). Specifically, in terms of added oils, the five diets were 100% FO (FO), 100%LO (LO100) and FO/LO in ratios of 3:1 (LO25), 1:1 (LO50) and 1:3 (LO75). Fish were sampled at 20 and 40 weeks, and samples of liver were collected for lipid analyses and total RNA extraction. Hepatocytes were also prepared and the activity of the HUFA biosynthetic pathway determined. Expression of fatty acid desaturase and elongase genes was determined by quantitative real time PCR and the ratio of the copy number of the targeted gene against that of β-actin was calculated. The results showed that after 20 weeks of feeding, desaturase and elongase gene expression in liver was increased in a graded manner by increasing dietary LO. Expression of both genes was positively and negatively correlated with dietary 18:3n-3 and n-3 HUFA, respectively. By 40 weeks of feeding, expression of neither gene showed the same degree of correlation with dietary fatty acid composition. In contrast, activity of the HUFA biosynthetic pathway, which showed some association with diet at 20 weeks, was positively and significantly correlated with dietary LO after 40 weeks of feeding. Elongation activity reflected the overall activity of the HUFA biosynthetic pathway to a greater degree than Δ5 desaturation activity. The possible mechanisms underlying the observed results and the regulation of the HUFA biosynthetic pathway are discussed.3

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“…The genes for Δ5-desaturase have been isolated from many organisms e.g. Caenorhabditis elegans (Michaelson et al 1998), various strain of Mortierella alpina (Tavares et al 2011), Rattus norvegicus (Zolfaghari et al 2001), Pavlova saliva (Zhou et al 2007), Naegleria gruberi (Fritz-Laylin et al 2010), Pythium irregulare (Hong et al 2002a), Homo sapiens (Cho et al 1999a), Octopus vulgaris (Monroig et al 2011) etc. Figure 4 shows the alignment of these sequences.…”

Section: δ5-desaturasementioning

confidence: 99%

Characterization of membrane-bound fatty acid desaturases

Klempová

Mihálik²,

Čertı́k³

2013

gpb

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Abstract. Membrane-bound desaturases play key role in metabolism of polyunsaturated fatty acids. Characterization of these enzymes and their genes is the first step in basic understanding of their proper functioning in living cells as well as in tailor-made preparation of highly-specific and highlyproductive strains of microorganisms interesting for applied biotechnology. It is also the crucial step in creation of transgenic agricultural crops with enhanced content of individual polyunsaturated fatty acids. Properties and applications of identified membrane-bound desaturases genes and enzymes are discussed in this review.

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Functional identification of a fatty acid Δ⁵ desaturase gene from Caenorhabditis elegans

Abstract: We have identified a cDNA from the nematode worm Caenorhabditis elegans that encodes a fatty acid v v S desaturase.

Cited by 81 publications

References 27 publications

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Effects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar)

Characterization of membrane-bound fatty acid desaturases

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Functional identification of a fatty acid Δ5 desaturase gene from Caenorhabditis elegans

Abstract: We have identified a cDNA from the nematode worm Caenorhabditis elegans that encodes a fatty acid v v S desaturase.

Cited by 81 publications

References 27 publications

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species

Effects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar)

Characterization of membrane-bound fatty acid desaturases

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Functional identification of a fatty acid Δ⁵ desaturase gene from Caenorhabditis elegans