Functional Identification and Characterization of the Brassica Napus Transcription Factor Gene BnAP2, the Ortholog of Arabidopsis Thaliana APETALA2

Yan, Xiaohong; Zhang, Lei; Chen, Bin; Xiong, Zhiyong; Chen, Chunli; Wang, Lijun; Yu, Jingyin; Liu, Changming; Wei, Wenhui

doi:10.1371/journal.pone.0033890

Cited by 25 publications

(28 citation statements)

References 76 publications

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“…The above four constructs were used to transform B. napus cultivar Zhongyou 821 by an Agrobacterium ‐mediated gene transformation method (Yan et al ., ). T 0 transformants were first subjected to Basta‐ or kanamycin‐based selection, and rooted well in selective medium.…”

Section: Resultsmentioning

confidence: 97%

Induction of telomere‐mediated chromosomal truncation and behavior of truncated chromosomes in Brassica napus

Yan

Yang

et al. 2017

The Plant Journal

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Engineered minichromosomes could be stably inherited and serve as a platform for simultaneously transferring and stably expressing multiple genes. Chromosomal truncation mediated by repeats of telomeric sequences is a promising approach for the generation of minichromosomes. In the present work, direct repetitive sequences of Arabidopsis telomere were used to study telomere-mediated truncation of chromosomes in Brassica napus. Transgenes containing alien Arabidopsis telomere were successfully obtained, and Southern blotting and fluorescence in situ hybridization (FISH) results show that the transgenes resulted in successful chromosomal truncation in B. napus. In addition, truncated chromosomes were inherited at rates lower than that predicted by Mendelian rules. To determine the potential manipulations and applications of the engineered chromosomes, such as the stacking of multiple transgenes and the Cre/lox and FRT/FLP recombination systems, both amenable to genetic manipulations through site-specific recombination in somatic cells, were tested for their ability to undergo recombination in B. napus. These results demonstrate that alien Arabidopsis telomere is able to mediate chromosomal truncation in B. napus. This technology would be feasible for chromosomal engineering and for studies on chromosome structure and function in B. napus.

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Section: Resultsmentioning

confidence: 97%

Induction of telomere‐mediated chromosomal truncation and behavior of truncated chromosomes in Brassica napus

Yan

Yang

et al. 2017

The Plant Journal

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“…This transcription factor belongs to the AP2/ERF family and is important for flower and seed development [ 71 ]. In Brassica napus , BnAP2 RNAi lines have delayed flower abscission and senescence [ 72 ]. In tomato, AP2 RNAi lines have altered levels of ethylene biosynthesis and differences in the timing of fruit ripening [ 73 ].…”

Section: Resultsmentioning

confidence: 99%

RNA-sequencing reveals early, dynamic transcriptome changes in the corollas of pollinated petunias

et al. 2014

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BackgroundPollination reduces flower longevity in many angiosperms by accelerating corolla senescence. This response requires hormone signaling between the floral organs and results in the degradation of macromolecules and organelles within the petals to allow for nutrient remobilization to developing seeds. To investigate early pollination-induced changes in petal gene expression, we utilized high-throughput sequencing to identify transcripts that were differentially expressed between corollas of pollinated Petunia × hybrida flowers and their unpollinated controls at 12, 18, and 24 hours after opening.ResultsIn total, close to 0.5 billion Illumina 101 bp reads were generated, de novo assembled, and annotated, resulting in an EST library of approximately 33 K genes. Over 4,700 unique, differentially expressed genes were identified using comparisons between the pollinated and unpollinated libraries followed by pairwise comparisons of pollinated libraries to unpollinated libraries from the same time point (i.e. 12-P/U, 18-P/U, and 24-P/U) in the Bioconductor R package DESeq2. Over 500 gene ontology terms were enriched. The response to auxin stimulus and response to 1-aminocyclopropane-1-carboxylic acid terms were enriched by 12 hours after pollination (hap). Using weighted gene correlation network analysis (WGCNA), three pollination-specific modules were identified. Module I had increased expression across pollinated corollas at 12, 18, and 24 h, and modules II and III had a peak of expression in pollinated corollas at 18 h. A total of 15 enriched KEGG pathways were identified. Many of the genes from these pathways were involved in metabolic processes or signaling. More than 300 differentially expressed transcription factors were identified.ConclusionsGene expression changes in corollas were detected within 12 hap, well before fertilization and corolla wilting or ethylene evolution. Significant changes in gene expression occurred at 18 hap, including the up-regulation of autophagy and down-regulation of ribosomal genes and genes involved in carbon fixation. This transcriptomic database will greatly expand the genetic resources available in petunia. Additionally, it will guide future research aimed at identifying the best targets for increasing flower longevity by delaying corolla senescence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0307-2) contains supplementary material, which is available to authorized users.

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“…Members of different subfamilies were reported to display distinct DNA-binding activities. Members of the AP2 subfamily could recognize the sequence GCAC (A/G) N (A/T) TCCC (A/G) ANG (C/T) (Krizek, 2003 ; Yan et al, 2012 ). Many DREB proteins had been shown to bind to a dehydration-responsive element (DRE)/C-repeat (CRT) element [A/G)CCGAC] (Park et al, 2001 ; Zhang et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

AP2/ERF Transcription Factor, Ii049, Positively Regulates Lignan Biosynthesis in Isatis indigotica through Activating Salicylic Acid Signaling and Lignan/Lignin Pathway Genes

Xiao

et al. 2017

Front. Plant Sci.

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Lignans, such as lariciresinol and its derivatives, have been identified as effective antiviral ingredients in Isatis indigotica . Evidence suggests that the APETALA2/ethylene response factor (AP2/ERF) family might be related to the biosynthesis of lignans in I. indigotica . However, the special role played by the AP2/ERF family in the metabolism and its underlying putative mechanism still need to be elucidated. One novel AP2/ERF gene, named Ii049 , was isolated and characterized from I. indigotica in this study. The quantitative real-time PCR analysis revealed that Ii049 was expressed highest in the root and responded to methyl jasmonate, salicylic acid (SA) and abscisic acid treatments to various degrees. Subcellular localization analysis indicated that Ii049 protein was localized in the nucleus. Knocking-down the expression of Ii049 caused a remarkable reduction of lignan/lignin contents and transcript levels of genes involved in the lignan/lignin biosynthetic pathway. Ii049 bound to the coupled element 1, RAV1AAT and CRTAREHVCBF2 motifs of genes IiPAL and IiCCR , the key structural genes in the lignan/lignin pathway. Furthermore, Ii049 was also essential for SA biosynthesis, and SA induced lignan accumulation in I. indigotica . Notably, the transgenic I. indigotica hairy roots overexpressing Ii049 showed high expression levels of lignan/lignin biosynthetic genes and SA content, resulting in significant accumulation of lignan/lignin. The best-engineered line (OVX049-10) produced 425.60 μg·g −1 lariciresinol, an 8.3-fold increase compared with the wild type production. This study revealed the function of Ii049 in regulating lignan/lignin biosynthesis, which had the potential to increase the content of valuable lignan/lignin in economically significant medicinal plants.

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Functional Identification and Characterization of the Brassica Napus Transcription Factor Gene BnAP2, the Ortholog of Arabidopsis Thaliana APETALA2

Cited by 25 publications

References 76 publications

Induction of telomere‐mediated chromosomal truncation and behavior of truncated chromosomes in Brassica napus

Induction of telomere‐mediated chromosomal truncation and behavior of truncated chromosomes in Brassica napus

RNA-sequencing reveals early, dynamic transcriptome changes in the corollas of pollinated petunias

AP2/ERF Transcription Factor, Ii049, Positively Regulates Lignan Biosynthesis in Isatis indigotica through Activating Salicylic Acid Signaling and Lignan/Lignin Pathway Genes

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