Functional expression of olfactory-adrenergic receptor chimeras and intracellular retention of heterologously expressed olfactory receptors

McClintock, Timothy S.; Landers, Teresa M.; Gimelbrant, Alexander A.; Fuller, Lynn Z.; Jackson, Brian A.; Jayawickreme, Channa; Lerner, Michael R.

doi:10.1016/s0169-328x(97)00099-5

Cited by 89 publications

(80 citation statements)

References 37 publications

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“…ORs do not traffic well to the plasma membrane when expressed in common cultured cell lines, in neuron-like cell lines, and even cell lines derived from the olfactory epithelium (11,12). This is consistent with the presumption that plasma membrane insertion is necessary for OR function and explains why ORs are difficult to functionally express in heterologous systems.…”

supporting

confidence: 67%

“…In contrast, the ␤ 2 -adrenergic receptor is expressed in the ER, the Golgi apparatus, endosomes, and on the plasma membrane (11,12). Because undifferentiated odora cells do not traffic ORs to the plasma membrane, we tested whether they also retained ORs in the ER.…”

Section: Undifferentiated Odora Cells Do Not Traffic Ors To Thementioning

confidence: 99%

“…Recombinant DNA Methods-Several of the constructs used were described previously (11); the OR constructs were OR5-GFP, U131-GFP, HA-OR5, and HA-U131, and the human ␤ 2 -adrenergic receptor constructs were ␤ 2 -adrenergic receptor-GFP and HA-␤ 2 -adrenergic receptor. Epitope-tagged receptors contain the hemagglutinin epitope tag (HA-1) at their N termini; the GFP constructs have receptors fused at their C termini to hGFP-S65T (obtained from CLONTECH; see Ref.…”

mentioning

confidence: 99%

“…Immunocytochemistry and Image Analysis-Procedures described previously for other cell types (11,12) were used for immunocytochemistry of odora cells. For cell staining prior to fixation, cells were rinsed with PBS (pH 7.3), incubated with primary antibodies (16B12) diluted at 1:300 in 1% BSA in PBS (solution B), then fixed with 3.7% paraformaldehyde, washed in PBS and incubated in solution B and with secondary antibodies (Cy3-conjugated anti-mouse, 1:300 in solution B).…”

mentioning

confidence: 99%

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Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

Gimelbrant

Haley

McClintock

2001

Journal of Biological Chemistry

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Olfactory receptors are difficult to functionally express in heterologous cells. They are typically retained in the endoplasmic reticulum of cells commonly used for functional expression studies and are only released to the plasma membrane in mature cells of the olfactory receptor neuron lineage. A recently developed olfactory cell line, odora, traffics olfactory receptors to the plasma membrane when differentiated. We found that undifferentiated odora cells do not traffic olfactory receptors to their surface, even though they release the receptors to the Golgi apparatus and endosomes. This behavior differs from other cell lines tested thus far. Differentiated odora cells also properly traffic vomeronasal receptors of the VN1 type, which lack sequence similarity to olfactory receptors. ODR-4, a protein that is necessary for plasma membrane trafficking of a chemosensory receptor in nematodes, facilitates trafficking of rat olfactory receptor U131 in odora and Chinese hamster ovary cells. Olfactory receptor trafficking from the endoplasmic reticulum to the plasma membrane involves at least two steps whose regulation depends on the maturation state of cells in the olfactory receptor neuron lineage. These results also indicate that some components of the regulatory mechanism are conserved.

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supporting

confidence: 67%

Section: Undifferentiated Odora Cells Do Not Traffic Ors To Thementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

Gimelbrant

Haley

McClintock

2001

Journal of Biological Chemistry

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“…It is known that OR proteins are usually retained in the endoplasmic reticulum (ER) and subsequently degraded in cultured cell lines of non-olfactory origins (7,8). Extensive efforts have been made to enhance the cell-surface expression of ORs in heterologous cells.…”

mentioning

confidence: 99%

Receptor-transporting Protein 1 Short (RTP1S) Mediates Translocation and Activation of Odorant Receptors by Acting through Multiple Steps

Pan

Chen

et al. 2012

Journal of Biological Chemistry

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Background: RTP1S facilitates the translocation of odorant receptors (ORs).Results: Different domains in RTP1S are important for different stages of OR trafficking, odorant-mediated responses, and interaction with ORs. Conclusion: RTP1S mediates the trafficking and ligand-induced response of ORs by acting through multiple steps. Significance: Probing the structure-function of RTP1S is crucial for understanding the mechanism of OR trafficking and activation.

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Taste, Chemical Biology of

Behrens

Stähler

Shi

et al. 2008

Wiley Encyclopedia of Chemical Biology

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The mammalian sense of taste is crucial for evaluating food palatability and nutritional quality. To achieve the detection of relevant chemicals, evolution has shaped a set of receptor molecules that allow the detection of five basic taste qualities: sweet, umami, bitter, salty, and sour. Each taste modality has unique characteristics and serves a distinct function for an animal's nutrition. Therefore, we address the characteristics, the recent advances, the persisting difficulties, and the future perspectives of the basic taste qualities in separate paragraphs. Enormous progress has been made in the identification and the characterization of taste receptor molecules, and in the growing number of animal genomes accessible from databases, which has inspired us to devote a section of this review to discuss a series of sophisticated evolutionary studies on taste receptor molecules. Finally, evidence is accumulating to show that taste receptor and signal transduction molecules have nongustatory functions as well. The extragustatory expression of such genes and the resulting implications are summarized in the final section.

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Functional expression of olfactory-adrenergic receptor chimeras and intracellular retention of heterologously expressed olfactory receptors

Cited by 89 publications

References 37 publications

Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

Olfactory Receptor Trafficking Involves Conserved Regulatory Steps

Receptor-transporting Protein 1 Short (RTP1S) Mediates Translocation and Activation of Odorant Receptors by Acting through Multiple Steps

Taste, Chemical Biology of

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