Functional evaluation of macrophages in fish from a polluted estuary

Weeks, B. A.; Warinner, J. Ernest

doi:10.1016/0165-2427(86)90135-2

Cited by 73 publications

(20 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies using this criterion have expressed phagocytosis simply as the percentage of cells which show any phagocytic activity. This is satisfactory in cases involving a very marked depression of macrophage activity, as in the study by Weeks and Warinner (1986) which reported a 3-4 fold decrease in the percentage of phagocytically active cells in fish from polluted water, but it may be rather insensitive in other situations. We have therefore expressed macrophage activity in terms of a phagocytic index, defined as the mean number of yeast cells engulfed per macrophage in a population of 200 macrophages.…”

Section: Discussionmentioning

confidence: 83%

The effect of various stresses, corticosteroids and adrenergic agents on phagocytosis in the rainbow trout Oncorhynchus mykiss

Narnaware

Baker

Tomlinson

1994

Fish Physiol Biochem

View full text Add to dashboard Cite

The effect of acute and chronic stress on the phagocytic activity of putative macrophages from the rainbow trout. Oncorhynchus mykiss has been assessed, using an in vitro phagocytic index, in which the average number of engulfed yeast cells in a population of phagocytes is determined. An injection stress given under light anaesthesia, or a longer noise stress combined with confinement, both significantly reduced, within 3 h, the level of phagocytic activity of macrophages from the spleen and pronephros. Daily injection stress over six days had a lesser effect on the proportion of phagocytically active cells even though plasma cortisol levels were equally raised. Daily dexamethasone injection depressed the proportion of phagocytically active cells more than saline injection. In these in vivo experiments, it was not possible to determine whether stress and steroids depressed the phagocytic activity of individual macrophages or caused the active macrophages to migrate out of the spleen and pronephros. Administration of cortisol (200 nM) to trout macrophages in vitro failed to depress phagocytic activity within a 3h period but both α- and β-adrenergic agonists (10 μM) were usually depressive. It is proposed that the autonomic nervous system may be an early regulator of macrophage phagocytosis following stress and that corticosteroids only exert their suppressive effect on macrophage activity in the longer term.

show abstract

Section: Discussionmentioning

confidence: 83%

The effect of various stresses, corticosteroids and adrenergic agents on phagocytosis in the rainbow trout Oncorhynchus mykiss

Narnaware

Baker

Tomlinson

1994

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…In the case of macrophage mediated innate immune function, bactericidal activity was depressed and respiratory burst activity was elevated in fish from the Ashtabula River. Decreased function of fish phagocytes is a well-documented consequence of contaminant exposure (Weeks and Warinner 1986;Rice and Schlenk 1995;Bols et al 2001;Lacroix et al 2001;Iwanowicz et al 2009). Respiratory burst activity is modulated by contaminants as well, but has been shown to increase or decrease depending on the chemical stimulus (Rice and Schlenk 1995;Rice et al 1996;Bols et al 2001;Regala et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River

et al. 2011

View full text Add to dashboard Cite

The use of fish as sentinels of aquatic ecosystem health is a biologically relevant approach to environmental monitoring and assessment. We examined the health of the Ashtabula River using histologic, immunologic, and endocrine biomarkers in brown bullhead (BB; Ameiurus nebulosus) and largemouth bass (Micropterus salmoides) and compared fish collected from a reference site (Conneaut Creek). Seasonal analysis was necessary to distinguish differences in fish between the two rivers. Overall BB from the Ashtabula River had a lower condition factor and significantly more macrophage aggregates than those from the reference site. Reduced bactericidal and cytotoxic-cell activity was observed in anterior kidney leukocytes from both BB and largemouth bass from the Ashtabula River. Lower plasma thyroxine and triiodo-L-thyronine in both species in the Ashtabula River indicated disruption of the thyroid axis. Differences in physiological biomarker responses were supported by body burden chemical concentrations when data were analyzed on a seasonal basis. The use of two fish species added a level of rigor that demonstrated biological effects were not exclusive to a single species. The results provide strong evidence that contaminants have affected fish in the Ashtabula River, a Great Lakes Area of Concern, and provide a baseline by which to evaluate remediation activities.

show abstract

“…PAHs have also contaminated both marine and freshwater systems [3,4]. One of the most toxic PAHs known and prototypical for the effects induced by this class of compounds is 3-methylcholanthrene (3-MC).…”

Section: Introductionmentioning

confidence: 99%

Possible implication of macrophages in the regulation of cytochrome P450 activities in carp (Cyprinus carpio)

Marionnet¹,

Deschaux²,

Reynaud³

2006

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Functional evaluation of macrophages in fish from a polluted estuary

Cited by 73 publications

References 16 publications

The effect of various stresses, corticosteroids and adrenergic agents on phagocytosis in the rainbow trout Oncorhynchus mykiss

The effect of various stresses, corticosteroids and adrenergic agents on phagocytosis in the rainbow trout Oncorhynchus mykiss

Histologic, immunologic and endocrine biomarkers indicate contaminant effects in fishes of the Ashtabula River

Possible implication of macrophages in the regulation of cytochrome P450 activities in carp (Cyprinus carpio)

Contact Info

Product

Resources

About