Functional Diversity of Microorganisms in Heavy Metal-Polluted Soils

Kenarova, Anelia; Boteva, Silvena

doi:10.1007/978-3-319-14526-6_13

Cited by 6 publications

(14 citation statements)

References 76 publications

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“…The MicroResp™ developed by Campbell et al (2003) is a method to study the carbon substrate utilization, which offers a rapid and sensitive method for the determination of differences in microbial community function (Creamer et al 2016;Chapman et al 2007;Burton et al 2010). MicroResp™ has been frequently used to determine the effects of contaminants such as heavy metals on soil catabolic functions (Bérard et al 2014;Kenarova and Boteva 2015). In our study, microbial catabolic activity measured by MicroResp™ was strengthened in the sub-layer rhizospheric soils due to the growth of S. alfredii, which was consistent with some previous studies.…”

Section: Effects Of S Alfredii Growth On Soil Microbial Metabolic Fumentioning

confidence: 99%

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Yang

Zhang

Lin

et al. 2017

Environ Sci Pollut Res

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The recovery of microbial community and activities is crucial to the remediation of contaminated soils. Distance-dependent variations of microbial community composition and metabolic characteristics in the rhizospheric soil of hyperaccumulator during phytoextraction are poorly understood. A 12-month phytoextraction experiment with Sedum alfredii in a Cd-contaminated soil was conducted. A pre-stratified rhizobox was used for separating sub-layer rhizospheric (0-2, 2-4, 4-6, 6-8, 8-10 mm from the root mat)/bulk soils. Soil microbial structure and function were analyzed by phospholipid fatty acid (PLFA) and MicroResp™ methods. The concentrations of total and specified PLFA biomarkers and the utilization rates for the 14 substrates (organic carbon) in the 0-2-mm sub-layer rhizospheric soil were significantly increased, as well as decreased with the increase in the distance from the root mat. Microbial structure measured by the ratios of different groups of PLFAs such as fungal/bacterial, monounsaturated/saturated, ratios of Gram-positive to Gram-negative (GP/GN) bacterial, and cyclopropyl/monoenoic precursors and 19:0 cyclo/18:1ω7c were significantly changed in the 0-2-mm soil. The PLFA contents and substrate utilization rates were negatively correlated with pH and total, acid-soluble, and reducible fractions of Cd, while positively correlated with labile carbon. The dynamics of microbial community were likely due to root exudates and Cd uptake by S. alfredii. This study revealed the stimulations and gradient changes of rhizosphere microbial community through phytoextraction, as reduced Cd concentration, pH, and increased labile carbons are due to the microbial community responses.

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Section: Effects Of S Alfredii Growth On Soil Microbial Metabolic Fumentioning

confidence: 99%

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Yang

Zhang

Lin

et al. 2017

Environ Sci Pollut Res

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“…This was likely due to metal stress inducing a variety of cellular detoxification mechanisms in microorganisms, including protein and amino acid macromolecules (Hall, 2002). This can be metabolized by microbial species that are better able to use amino acids than other carbon sources (Kenarova and Boteva, 2015). The effect of HMs on microbial functional diversity is lower with low to moderate soil contamination (Kenarova et al, 2014), and diversity is substantially reduced under high HMs contamination (Gremion et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Structural and functional characteristics of soil microbial communities in response to different ecological risk levels of heavy metals

Chen

Zhang

et al. 2022

Front. Microbiol.

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ObjectiveThe potential ecological risk index (RI) is the most commonly used method to assess heavy metals (HMs) contamination in soils. However, studies have focused on the response of soil microorganisms to different concentrations, whereas little is known about the responses of the microbial community structures and functions to HMs at different RI levels.MethodsHere, we conducted soil microcosms with low (L), medium (M) and high (H) RI levels, depending on the Pb and Cd concentrations, were conducted. The original soil was used as the control (CK). High-throughput sequencing, qPCR, and Biolog plate approaches were applied to investigate the microbial community structures, abundance, diversity, metabolic capacity, functional genes, and community assembly processes.ResultThe abundance and alpha diversity indices for the bacteria at different RI levels were significantly lower than those of the CK. Meanwhile, the abundance and ACE index for the fungi increased significantly with RI levels. Acidobacteria, Basidiomycota and Planctomycetes were enriched as the RI level increased. Keystone taxa and co-occurrence pattern analysis showed that rare taxa play a vital role in the stability and function of the microbial community at different RI levels. Network analysis indicates that not only did the complexity and vulnerability of microbial community decrease as risk levels increased, but that the lowest number of keystone taxa was found at the H level. However, the microbial community showed enhanced intraspecific cooperation to adapt to the HMs stress. The Biolog plate data suggested that the average well color development (AWCD) reduced significantly with RI levels in bacteria, whereas the fungal AWCD was dramatically reduced only at the H level. The functional diversity indices and gene abundance for the microorganisms at the H level were significantly lower than those the CK. In addition, microbial community assembly tended to be more stochastic with an increase in RI levels.ConclusionOur results provide new insight into the ecological impacts of HMs on the soil microbiome at different risk levels, and will aid in future risk assessments for Pb and Cd contamination.

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“…Heavy metals are dangerous because they tend to bio-accumulate [6]. Bioaccumulation is an increase in the concentration of chemical elements in a biological organism over time, as opposed to its concentration in the environment [6], [8]. In the natural environment, their concentration is low.…”

Section: Introductionmentioning

confidence: 99%

“…In the natural environment, their concentration is low. Whereas high concentrations of heavy metals in the case of contaminated environments result in environmental and public health impacts [3], [8].…”

Section: Introductionmentioning

confidence: 99%

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Microbial and Heavy Metal Presence and Impact on Soil Pollution for Central Albania

Jançe

Bogoev

2023

ICSIS

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This scientific study was undertaken by presenting data on the presence of microorganisms (bacteria) and heavy metals found in the underground layers of Elbasan city. From the obtained results, it was judged whether the presence of bacteria and chemical elements is at such levels as to be considered as a soil pollution factor. The main purpose of this scientific paper is to provide the possible correlation between chemical and microbial soil pollution, which directly or indirectly affects the health of the city's residents. To achieve this goal, five soil samples were taken by digging to a depth of 0.5 m, during March 2023. Mainly, the Elbasan area has been at the center of criticism over the years and is considered an area with significant atmospheric pollution, of a chemical and microbiological nature. We have obtained results that prove chemical and bacterial data that can be considered as a soil pollution factor. In conclusion, based on the analysis of the results and compared with the permitted rates of pollution, the level of chemical pollution of the soil from the nickel element appears, which is in the amount of about 2 and a half times more than the standards determined by the relevant Regulatory Acts of the EU. Based on the study and monitoring that was done to the Elbasan city, we think that soil pollution is mainly dedicated to the activity of the farmer in the misuse of organic and chemical fertilizers, the mismanagement of wastewater by the relevant authorities, and especially the abusive activity to the detriment of nature carried out by industries that operate in the city.

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Functional Diversity of Microorganisms in Heavy Metal-Polluted Soils

Cited by 6 publications

References 76 publications

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Distance-dependent varieties of microbial community structure and metabolic functions in the rhizosphere of Sedum alfredii Hance during phytoextraction of a cadmium-contaminated soil

Structural and functional characteristics of soil microbial communities in response to different ecological risk levels of heavy metals

Microbial and Heavy Metal Presence and Impact on Soil Pollution for Central Albania

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