1999
DOI: 10.1002/(sici)1097-0169(199909)44:1<1::aid-cm1>3.0.co;2-8
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Functional dissection of nebulette demonstrates actin binding of nebulin-like repeats and Z-line targeting of SH3 and linker domains

Abstract: Nebulette, a 107 kDa protein associated with the I‐Z‐I complex of cardiac myofibrils, may play an important role in the assembly of the Z‐line. Determination of the complete primary structure of 1011 residue human fetal nebulette reveals a four‐domain layout similar to skeletal muscle nebulin: a short N‐terminal domain, followed by 22 nebulin‐like repeats that are linked to a C‐terminal Src homology 3 (SH3) domain via a short linker domain. To elucidate the mechanisms of assembly for nebulette in the Z‐line, t… Show more

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Cited by 52 publications
(80 citation statements)
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References 57 publications
(101 reference statements)
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“…This was in contrast to the MSN fragment, which was confined to the inclusion bodies upon large scale expression in E. coli (data not shown), similar to previously published data on mammalian nebulin fragments (6,8,24). A mouse polyclonal antiserum was generated using the purified CN5 protein as the immunogen.…”
Section: Figsupporting
confidence: 86%
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“…This was in contrast to the MSN fragment, which was confined to the inclusion bodies upon large scale expression in E. coli (data not shown), similar to previously published data on mammalian nebulin fragments (6,8,24). A mouse polyclonal antiserum was generated using the purified CN5 protein as the immunogen.…”
Section: Figsupporting
confidence: 86%
“…Evidence has been mounted by studies showing the interaction of nebulin or nebulette fragments with F-actin (6,8), protein constituents of the Z-line (6,9), and with tropomodulin at the pointed ends of the thin filaments (11). Correlations between nebulin/nebulette expression and Z-line thickness as well as nebulin protein size and actin filament length in skeletal muscle sarcomeres (5,26) have all contributed to the large body of evidence in support of a structural role for nebulin/nebulette.…”
Section: Discussionmentioning
confidence: 99%
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