Functional contribution of mesencephalic locomotor region nuclei to locomotor recovery after spinal cord injury

Roussel, Marie; Lafrance-Zoubga, David; Josset, Nicolas; Lemieux, Maxime; Bretzner, Frédéric

doi:10.1016/j.xcrm.2023.100946

Cited by 8 publications

(13 citation statements)

References 84 publications

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“…Given their excitatory reticulospinal drive on motor control and locomotion 7 , we focused in the current study on glutamatergic neurons of the MRF. As previously shown in intact settings 7,20 and more recently in SCI mice 27 , using a paradigm of 10 ms photostimulation pulses allowed us to probe changes in reticulospinal e cacy over time at rest and during locomotion after SCI.…”

Section: Plasticity Of the Reticulospinal Drive Of Medullary Glutamat...mentioning

confidence: 98%

“…Kinematic recording was obtained at a frequency of 250 frames s -1 on both sides. As previously described 7,20,27 , percutaneous EMG recordings of the Tibialis anterior and Gastrocnemius lateralis on both sides were acquired with insulated zinc-chromium 50 µm wires in a duplex con guration ampli ed with Model 3600 extracellular ampli er (A-M Systems) and recorded on Spike2 (Cambridge Eletronic Design).…”

Section: Kinematic and Recordingsmentioning

confidence: 99%

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Functional contribution of glutamatergic neurons of medullary reticular nuclei to locomotor recovery after spinal cord injury

Bretzner¹,

Lemieux

Karimi³

2023

Preprint

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Spinal cord injury (SCI) disrupts the descending drive from the brain and causes a range of deficits. We have investigated the functional plasticity of the glutamatergic reticulospinal drive of the medullary reticular formation (MRF) after a lateral thoracic hemisection in mice. The excitatory descending drive was impaired after SCI, independently of its origin. The direction of functional plasticity was anti-correlated with its pre-injury efficacy. Stimulation of sites in the most dorsal MRF decelerated or stopped locomotion, whereas the most ventral sites increased speed. The ability to steer was preserved with modest impairment. Activating the descending MRF drive improved stepping ability of chronically impaired SCI mice and its conditioning improved recovery of skilled locomotion. These results highlight the resilience and capacity for reorganization of the glutamatergic reticulospinal command after SCI, along with its suitability as a therapeutical target to promote functional recovery.

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Section: Plasticity Of the Reticulospinal Drive Of Medullary Glutamat...mentioning

confidence: 98%

Section: Kinematic and Recordingsmentioning

confidence: 99%

Functional contribution of glutamatergic neurons of medullary reticular nuclei to locomotor recovery after spinal cord injury

Bretzner¹,

Lemieux

Karimi³

2023

Preprint

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“…Now, combining mouse genetics and activation and ablation strategies alongside sensitive outcome measures, Roussel et al. 1 aim to probe and pinpoint any distinction with greater accuracy.…”

Section: Main Textmentioning

confidence: 99%

“…Roussel et al. 1 first focused on this spontaneous recovery following a thoracic lateral hemisection model of spinal cord injury. This is a one-sided transection lesion of the spinal cord, abolishing the MLR input on that same (left, ipsilesional) side but largely sparing that coming from the contralesional MLR.…”

Section: Main Textmentioning

confidence: 99%

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Mesencephalic locomotor region stimulation—cuneiform or pedunculopontine?

Burnside

Bradke

2023

Cell Reports Medicine

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Functional plasticity of glutamatergic neurons of medullary reticular nuclei after spinal cord injury in mice

Lemieux,

Karimi,

Bretzner

2024

Nat Commun

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Spinal cord injury disrupts the descending command from the brain and causes a range of motor deficits. Here, we use optogenetic tools to investigate the functional plasticity of the glutamatergic reticulospinal drive of the medullary reticular formation after a lateral thoracic hemisection in female mice. Sites evoking stronger excitatory descending drive in intact conditions are the most impaired after injury, whereas those associated with a weaker drive are potentiated. After lesion, pro- and anti-locomotor activities (that is, initiation/acceleration versus stop/deceleration) are overall preserved. Activating the descending reticulospinal drive improves stepping ability on a flat surface of chronically impaired injured mice, and its priming enhances recovery of skilled locomotion on a horizontal ladder. This study highlights the resilience and capacity for reorganization of the glutamatergic reticulospinal command after injury, along with its suitability as a therapeutical target to promote functional recovery.

show abstract

Functional contribution of mesencephalic locomotor region nuclei to locomotor recovery after spinal cord injury

Cited by 8 publications

References 84 publications

Functional contribution of glutamatergic neurons of medullary reticular nuclei to locomotor recovery after spinal cord injury

Functional contribution of glutamatergic neurons of medullary reticular nuclei to locomotor recovery after spinal cord injury

Mesencephalic locomotor region stimulation—cuneiform or pedunculopontine?

Functional plasticity of glutamatergic neurons of medullary reticular nuclei after spinal cord injury in mice

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