Functional classification of the microbial feruloyl esterases

Crepin, Valérie F.; Faulds, Craig B.; Connerton, Ian F.

doi:10.1007/s00253-003-1476-3

Cited by 310 publications

(217 citation statements)

References 50 publications

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“…Crepin et al 28) proposed to classify FAEs into four groups depending on either their substrate specificities toward four synthetic methyl esters of hydroxycinnamic acids (ferulic acid, p coumaric acid, sinapinic acid and caffeic acid) or their primary protein structure. Type A enzymes such as A. niger FAE II 37) and FAE III 38) are inactive towards MCA.…”

Section: Discussionmentioning

confidence: 99%

“…chlorogenate esterase and A. oryzae tannase. 28) FAE 1 had the highest enzyme activity at 50 C. The thermostability of the enzyme was determined by incubating it at various temperatures at pH 5.0 and measuring residual activity. After incubation of the enzyme at pH 5.0 at 40 C for 1 h, the initial activity of the enzyme completely remained.…”

Section: Selection Of Fae-producing Strainsmentioning

confidence: 99%

See 1 more Smart Citation

Efficient Extraction of Ferulic Acid from Sugar Beet Pulp Using the Culture Supernatant of Penicillium chrysogenum

et al. 2005

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Ferulic acids have been found in the cell walls of many plant products including maize bran, 1) sugar cane bagasse,2) wheat bran, 3) sugar beet pulp, 4,5) and spinach. 6) In the three former tissues, ferulic acid is esterified to the C 5 of arabinose in arabinoxylan, a 1,4 D xylan to which L arabinofuranosyl residues are attached at position 2 or 3. In contrast, ferulic acids mainly attach to the C 2 position of 1,5 linked arabinofuranose residues or the C 6 of 1,4 linked galactopyranose residues in the hairy region of pectins of sugar beet and spinach. 7,8) Recently, Levigne et al. demonstrated that ferulic acid is also linked to the C 5 position of arabinofuranose residues in sugar beet pectin by analysis of the structures of feruloylated oligosaccharides obtained by treating sugar beets with a commercial enzyme preparation (Driselase). 9) Furthermore, some ferulic acids exist as dehydrodimers (e.g. 5 5 dehydrodiferulic acid) in the cell walls of several plant species, which are formed through an oxidation reaction catalyzed by peroxidases.10,11) Possible roles of ferulic acid in plant cell walls are to decrease the digestibility of the cell wall by microorganisms 12) and to regulate cell growth 13) by cross linking cell wall polysaccharides.Ferulic acid esterases (FAEs) are enzymes that catalyze the hydrolysis of ester linkages between ferulic acids and sugars or alcohols. The number of studies concerning microbial FAEs has been increasing in the last ten years and many FAEs have been isolated and characterized. In the utilization of agro industrial wastes such as sugar beet pulp, effective degradation of plant cell walls requires FAEs for the release of ferulic acid as well as polysaccharidases such as xylanases 14) and arabinanases. 15) Moreover, ferulic acid has several potential applications: it may be useful as an anti oxidizing agent, 16,17) an anti inflammatory drug, 18,19) and a food preservative that inhibits microbial growth. 20,21) Enzymatic extraction of ferulic acid from industrial wastes has been studied. Penicillium funiculosum FAEB releases 98% of esterified ferulic acid from wheat bran in the presence of xylanase and releases 35% of esterified ferulic acid from sugar beet pulp in the presence of a mixture of endo arabinanase and L arabinofuranosidase. 22) To the best of our knowledge, that releases more ferulic acid from sugar beet pulp than any other enzyme.In this paper we describe the screening of microorganisms that can achieve high yield extraction of free ferulic acid from sugar beet pulp. In addition, we describe the isolation and some of the characteristics of a novel FAE, termed FAE 1, produced by P. chrysogenum 31B. MATERIALS AND METHODS Chemicals and reagents.ResourceQ 6 mL, ResourceS 6 mL, MonoQ HR 5 5, MonoS HR 5 5 and HiLoad 16 60 Superdex 75 columns were purchased from Amersham Biosciences. All other chemicals were from Wako Pure Chemical Industries, Ltd. (Osaka) unless otherwise stated, and were of certified reagent grade.Organism and cultivation conditions. The microorganisms...

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Section: Discussionmentioning

confidence: 99%

Section: Selection Of Fae-producing Strainsmentioning

confidence: 99%

Efficient Extraction of Ferulic Acid from Sugar Beet Pulp Using the Culture Supernatant of Penicillium chrysogenum

et al. 2005

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“…This observation was further confirmed by substrate profiling (using p-nitrophenyl esters of different chain lengths) which revealed that Fae6 prefers short to medium substrate chain lengths, a characteristic typical of true carboxylesterases (5). The application of Crepin's feruloyl esterases classification scheme (6) (6). However, due to the lack of the availability of the FAX and FAXX substrates, we could not study the ability of Fae6 in releasing ferulic acid dimmers, which would have allowed Fae6 to be defined into a specific classification category.…”

Section: Discussionmentioning

confidence: 99%

“…Like other carboxyl ester hydrolases, all reported FAEs are serine hydrolases, with α/β fold tertiary structures (5). FAEs are sub-classified into four types (type A-D), based on the amino acid sequence identities, substrate specificity profiles against methyl esters of hydroxycinnamic acids and the varying ability to release diferulic acids from esterified substrates (6). The applications of FAEs in the industry cover a broad spectrum, including in the food industry as food additives (7), the pharmaceutical industry for the synthesis of bioactive phytochemicals (8), cosmetic industry for the production of flavour and fragment precursors (9), in animal feeds as feed additives (10,11) and in pulp and paper industry as bleaching agents (12).…”

Section: Introductionmentioning

confidence: 99%

A feruloyl esterase derived from a leachate metagenome library

Rashamuse¹,

Sanyika²,

Ronneburg³

et al. 2012

BMB Reports

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“…13) Then, FAE has been classified into four types (types A-D) based on differences in substrate specificity (preferential hydrolysis of methyl p-coumarate, methyl caffeate, methyl ferulate, or methyl sinapate) and effectiveness in releasing diferulic acid. 14,15) Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) is a high cellulolyticenzyme producing fungus first isolated by Yamanobe et al 16) There is no report for FAE activity of T. cellulolyticus. We identified the feruloyl esterase B homologous gene (faeB) by searching the draft genome sequence of T. cellulolyticus.…”

mentioning

confidence: 99%

Characterization of a feruloyl esterase B from Talaromyces cellulolyticus

Watanabe

Yoshida

Fukada

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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A feruloyl esterase catalyzes the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from esterified sugars in plant cell walls. Talaromyces cellulolyticus is a high cellulolytic-enzyme producing fungus. However, there is no report for feruloyl esterase activity of T. cellulolyticus. Analysis of the genome database of T. cellulolyticus identified a gene encoding a putative feruloyl esterase B. The recombinant enzyme was prepared using a T. cellulolyticus homologous expression system and characterized. The purified enzyme exhibited hydrolytic activity toward p-nitrophenyl acetate, p-nitrophenyl trans-ferulate, methyl ferulate, rice husk, and bagasse. HPLC assays showed that the enzyme released ferulic acid and p-coumaric acid from hydrothermal-treated rice husk and bagasse. Trichoderma sp. is well-known high cellulolytic-enzyme producing fungus useful for the lignocellulosic biomass saccharification. Interestingly, no feruloyl esterase has been reported from Trichoderma sp. The results show that this enzyme is expected to be industrially useful for biomass saccharification.

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Functional classification of the microbial feruloyl esterases

Cited by 310 publications

References 50 publications

Efficient Extraction of Ferulic Acid from Sugar Beet Pulp Using the Culture Supernatant of Penicillium chrysogenum

Efficient Extraction of Ferulic Acid from Sugar Beet Pulp Using the Culture Supernatant of Penicillium chrysogenum

A feruloyl esterase derived from a leachate metagenome library

Characterization of a feruloyl esterase B from Talaromyces cellulolyticus

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