Characterization of a feruloyl esterase B from Talaromyces cellulolyticus

Abstract

A feruloyl esterase catalyzes the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from esterified sugars in plant cell walls. Talaromyces cellulolyticus is a high cellulolytic-enzyme producing fungus. However, there is no report for feruloyl esterase activity of T. cellulolyticus. Analysis of the genome database of T. cellulolyticus identified a gene encoding a putative feruloyl esterase B. The recombinant enzyme was prepared using a T. cellulolyticus homologous expression system and characterized. The purified enzyme exhibited hydrolytic activity toward p-nitrophenyl acetate, p-nitrophenyl trans-ferulate, methyl ferulate, rice husk, and bagasse. HPLC assays showed that the enzyme released ferulic acid and p-coumaric acid from hydrothermal-treated rice husk and bagasse. Trichoderma sp. is well-known high cellulolytic-enzyme producing fungus useful for the lignocellulosic biomass saccharification. Interestingly, no feruloyl esterase has been reported from Trichoderma sp. The results show that this enzyme is expected to be industrially useful for biomass saccharification.

Talaromyces borbonicus, sp. nov., a novel fungus from biodegraded Arundo donax with potential abilities in lignocellulose conversion

“…Strains belonging to the Talaromyces genus have an enormous biotechnological potential because of their ability to secrete a variety of small molecules and enzymes with different applications, such as xylanases for pulp bleaching Maalej-Achouri et al 2009;Orencio-Trejo et al 2016), cellulases for lignocellulosic biomass deconstruction (Inoue et al 2014;Fujii et al 2015;Schafhauser et al 2015;Orencio-Trejo et al 2016), feruloyl esterases for agro-food industries and tailored synthesis of pharmaceuticals (Crepin et al 2003;Garcia-Conesa et al 2004;Vafiadi et al 2006;Mandalari et al 2008;Watanabe et al 2015) and pigments for the sustainable bioproduction of environmentally friendly dyes (Schafhauser et al 2015). In this work, a Talaromyces isolate from the lignocellulosic biomass A. donax during biodegradation under natural conditions (Ventorino et al 2015) in the Gussone Park of the Royal Palace of Portici, Naples, Italy, was studied.…”

AtSYP71 Is Important for Plant Cell Wall Biosynthesis and Stress Adaptation.

“…On the other hand, the cycling and vacuolar degradation pathways coordinately ne regulate homeostasis of the PM-localized proteins, especially under environmental stimuli [11,18]. The small CesA compartments (SmaCCs) derived from the Golgi deliver CSCs to the PM during PCW and SCW deposition [33,91]. SmaCCs are also implicated in CesAs endocytosis and cycling between the TGN and PM [9,11,25,33,92,93].…”

Cross-Linked Enzyme Aggregates of Feruloyl Esterase Preparations from Thermothelomyces thermophila and Talaromyces wortmannii

“…FA is widely known for its antioxidant properties, and for this purpose, it is used today in cosmetic products, such as C E FERULIC ® Serum by the company SkinCeuticals, as a food preservative [13] and as a building block for the production of several chemicals, such as polymers, protocatechuic acid derivatives and catechol derivatives, mainly vanillin [14]. Therefore, the widespread industrial use of FA has led to significant research efforts for the discovery, characterization, heterologous expression and mode of action of several FAEs [14][15][16][17], aiming at the complete elucidation of their action mechanism, on the one hand, and at the development of industrially-attractive biocatalyst preparations, on the other. The main focus of the present work was the improvement of the stability and reusability properties of eight different commercial FAE preparations, through CLEA immobilization.…”