2020
DOI: 10.1038/s41598-020-68789-3
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Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family

Abstract: Bacterial alginate initially consists of 1–4-linked β-D-mannuronic acid residues (M) which can be later epimerized to α- L -guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii has been extensively studied, and three genes putatively encoding AlgE-type epimerases have recently been identified in the genome of Azotobacter chroococcum . The three A. chroococcum genes, here designate… Show more

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Cited by 22 publications
(33 citation statements)
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“…The product pattern changed somewhat, especially on polyMG where NaCl enhances epimerization (Figure S4 and Table S1). 13 C NMRexperiments on polyM without NaCl showed the same trends as discussed here, except for a total lack of MGG-signals during the reaction course. Lyase activity seems to be more dominating in the absence of salt, and this was investigated in more detail (see later section).…”
Section: The Lyase and Epimerization Activities Are Mutually Dependentsupporting
confidence: 77%
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“…The product pattern changed somewhat, especially on polyMG where NaCl enhances epimerization (Figure S4 and Table S1). 13 C NMRexperiments on polyM without NaCl showed the same trends as discussed here, except for a total lack of MGG-signals during the reaction course. Lyase activity seems to be more dominating in the absence of salt, and this was investigated in more detail (see later section).…”
Section: The Lyase and Epimerization Activities Are Mutually Dependentsupporting
confidence: 77%
“…The epimerization pattern and preferred cleavage sites of AlgE7 have been studied previously (11,23), but the mode of action is not understood. To verify the end products and further elucidate the mode of action, we have used 1 H NMR at different reaction time points, timeresolved 13 C NMR, and 13 C HSQC (Heteronuclear single quantum coherence spectroscopy) spectra (Figure 2, Figure S1, and Table S1). The lyase and epimerase activities are closely linked in how they affect each other in the creation of the product pattern.…”
Section: The Lyase and Epimerization Activities Are Mutually Dependentmentioning
confidence: 99%
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