Functional Characterization of the slr1944 Gene of Cyanobacterium Synechocystis sp. PCC 6803

Serebriiskaya, T. S.; Los, Dmitry A.

doi:10.1023/b:rupp.0000047826.13738.e1

Cited by 3 publications

(1 citation statement)

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“…The accumulation of FFAs could be attributed to enzymes such as lipases that can degrade phospholipids into FFAs. So far, two putative lipases have been identified in Synechocystis (lipA, sll1969 [34] and slr1994 [41]). In our study, we found that lipA was significantly up-regulated in our ∆aas strains during the dark phase (Table S5B).…”

Section: Fatty Acid Extraction and Derivatizationmentioning

confidence: 99%

Diel light:dark cycles significantly reduce FFA accumulation in FFA producing mutants of Synechocystis sp. PCC 6803 compared to continuous light

et al. 2015

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We examine the effect of diel light:dark cycles (12:12 LD) on the production of free fatty acids (FFAs) in Synechocystis sp. PCC 6803. Our results show that FFA titers in FFA producing strains of Synechocystis were significantly reduced in 12:12 LD compared to continuous light. On the transcriptional level, we saw no significant changes of the fatty acid biosynthesis pathway as a result of increased FFA production. In addition, we describe our efforts in increasing FFA production by introducing 'tesA from E. coli. Despite evidence of 'tesA mRNA transcript abundance, no measurable TesA enzymatic activity was observed in our Synechocystis mutants. Overall, our work demonstrates the importance of considering the effects of diel light:dark cycles in the metabolic engineering of cyanobacteria.

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Section: Fatty Acid Extraction and Derivatizationmentioning

confidence: 99%