Polyphosphate accumulation dynamics in a population of Synechocystis sp. PCC 6803 cells under phosphate overplus

Воронков, А. С.; Sinetova, Maria A.

doi:10.1007/s00709-019-01374-2

Cited by 26 publications

(17 citation statements)

References 50 publications

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“…This risk is mitigated when a considerable part of the P i taken up by the cell during LPU is converted into PolyP. Although the cell reserves of PolyP were depleted after P starvation, there were cells with measurable levels of PolyP ( Figure 5 ), which is typical for P-starving cyanobacterial cultures [ 70 ]. In such a situation, cyanobacteria rapidly convert the absorbed P i into PolyP, the main P reserve in the cell.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Solovchenko

Gorelova

Карпова

et al. 2020

Cells

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To cope with fluctuating phosphorus (P) availability, cyanobacteria developed diverse acclimations, including luxury P uptake (LPU)—taking up P in excess of the current metabolic demand. LPU is underexplored, despite its importance for nutrient-driven rearrangements in aquatic ecosystems. We studied the LPU after the refeeding of P-deprived cyanobacterium Nostoc sp. PCC 7118 with inorganic phosphate (Pi), including the kinetics of Pi uptake, turnover of polyphosphate, cell ultrastructure, and gene expression. The P-deprived cells deployed acclimations to P shortage (reduction of photosynthetic apparatus and mobilization of cell P reserves). The P-starved cells capable of LPU exhibited a biphasic kinetic of the Pi uptake and polyphosphate formation. The first (fast) phase (1–2 h after Pi refeeding) occurred independently of light and temperature. It was accompanied by a transient accumulation of polyphosphate, still upregulated genes encoding high-affinity Pi transporters, and an ATP-dependent polyphosphate kinase. During the second (slow) phase, recovery from P starvation was accompanied by the downregulation of these genes. Our study revealed no specific acclimation to ample P conditions in Nostoc sp. PCC 7118. We conclude that the observed LPU phenomenon does not likely result from the activation of a mechanism specific for ample P conditions. On the contrary, it stems from slow disengagement of the low-P responses after the abrupt transition from low-P to ample P conditions.

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Section: Discussionmentioning

confidence: 99%

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Solovchenko

Gorelova

Карпова

et al. 2020

Cells

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“…The accumulation of polyP during luxury uptake is considered important for protection of aquatic organisms against future exposure to P limitation ( Hupfer et al., 2004 ; McMahon and Read, 2013 ). For Synechocystis PCC6803, PO 4 3- uptake and polyP accumulation by cells exposed to overplus conditions resulted in the initial detection of polyP granules in most cells within 3 min of replenishing the medium with PO 4 3- , with the number of cells containing these granules sustained for about 1 h ( Voronkov and Sinetova, 2019 ) followed by degradation and redistributed of the polyP within the cell over a period of a few to several days. Additionally, polyP accumulation in Synechocystis occurred to a lesser extent in the dark than in the light and did not appear to be very sensitive to temperature changes.…”

Section: Polyp Functions and Regulation In Photosynthetic Microbesmentioning

confidence: 99%

Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae

2020

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Polyphosphate (polyP), a polymer of orthophosphate (PO 4 3-) of varying lengths, has been identified in all kingdoms of life. It can serve as a source of chemical bond energy (phosphoanhydride bond) that may have been used by biological systems prior to the evolution of ATP. Intracellular polyP is mainly stored as granules in specific vacuoles called acidocalcisomes, and its synthesis and accumulation appear to impact a myriad of cellular functions. It serves as a reservoir for inorganic PO 4 3and an energy source for fueling cellular metabolism, participates in maintaining adenylate and metal cation homeostasis, functions as a scaffold for sequestering cations, exhibits chaperone function, covalently binds to proteins to modify their activity, and enables normal acclimation of cells to stress conditions. PolyP also appears to have a role in symbiotic and parasitic associations, and in higher eukaryotes, low polyP levels seem to impact cancerous proliferation, apoptosis, procoagulant and proinflammatory responses and cause defects in TOR signaling. In this review, we discuss the metabolism, storage, and function of polyP in photosynthetic microbes, which mostly includes research on green algae and cyanobacteria. We focus on factors that impact polyP synthesis, specific enzymes required for its synthesis and degradation, sequestration of polyP in acidocalcisomes, its role in cellular energetics, acclimation processes, and metal homeostasis, and then transition to its potential applications for bioremediation and medical purposes.

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“…2a ), whereby P was accumulated to a significant part as polyP, which is characteristic for overplus uptake [ 25 ]. Many different types of oligotrophic aquatic habitats experience only temporal P pulses, e.g., from redox changes at the benthic interface leading to P release from the sediment [ 32 ], storm run-off [ 28 ], upwelling [ 46 ], or excretions of aquatic animals [ 47 ]. The capability of microorganisms to immediately take up, store, and efficiently re-use this P by overplus uptake is hence of critical importance for a population to sustain a potential subsequent period of low P availability.…”

Section: Discussionmentioning

confidence: 99%

Response of cyanobacterial mats to ambient phosphate fluctuations: phosphorus cycling, polyphosphate accumulation and stoichiometric flexibility

Jentzsch

Grossart

Plewe

et al. 2023

ISME Communications

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Cyanobacterial mats inhabit a variety of aquatic habitats, including the most extreme environments on Earth. They can thrive in a wide range of phosphorus (P) levels and are thus important players for ecosystem primary production and P cycling at the sediment-water interface. Polyphosphate (polyP), the major microbial P storage molecule, is assigned a critical role in compensating for phosphate fluctuations in planktonic cyanobacteria, but little is known about potentially analogous mechanisms of mat-forming cyanobacteria. To investigate acclimation strategies of cyanobacterial mats to fluctuating phosphate concentrations, laboratory batch experiments were conducted, in which the cosmopolitan mat-forming, marine cyanobacterium Sodalinema stali was exposed to low dissolved P concentrations, followed by a P pulse. Our results show that the cyanobacteria dynamically adjusted cellular P content to ambient phosphate concentrations and that they had accumulated polyP during periods of high phosphate availability, which was subsequently recycled to sustain growth during phosphate scarcity. However, following the depletion of dispensable cellular P sources, including polyP, we observed a reallocation of P contained in DNA into polyP, accompanied by increasing alkaline phosphatase activity. This suggests a change of the metabolic focus from growth towards maintenance and the attempt to acquire organic P, which would be naturally contained in the sediment. P overplus uptake following a simulated P pulse further suggests that Sodalinema-dominated mats exhibit elaborated mechanisms to cope with severe P fluctuations to overcome unfavourable environmental conditions, and potentially modulate critical P fluxes in the aquatic cycle.

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Polyphosphate accumulation dynamics in a population of Synechocystis sp. PCC 6803 cells under phosphate overplus

Cited by 26 publications

References 50 publications

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae

Response of cyanobacterial mats to ambient phosphate fluctuations: phosphorus cycling, polyphosphate accumulation and stoichiometric flexibility

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