Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli

Gao, Jie; Lan, Ting

doi:10.1038/srep19467

Cited by 90 publications

(71 citation statements)

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“…The transformed E. coli (pET28a- DofLEA ) cell were grown in our lab as described previously103037. Briefly, DofLEA genes were inserted into the pET28a vector and then transformed into the E. coli host strain BL21 (DE3).…”

Section: Methodsmentioning

confidence: 99%

“…Heat and salt tolerance assays were performed as described previously103037 (shown in Figure 6S). The transformed E. coli (pET28a- DofLEA ) colonies grown on Luria-Bertani (LB)+ kanamycin plates were used to inoculate starting cultures, which were incubated at 37 °C for 12–16 h. The cultures were diluted 50-fold using fresh LB medium, and incubation continued at 37 °C until the mid-log phase (2–3 h, OD 600 = 0.6).…”

Section: Methodsmentioning

confidence: 99%

“…The viability ratio of the transformants under heat and salt conditions was calculated according to the following formula10: Cell viability ratio = (colony number on stressed plate/colony number on unstressed plate) × 100%. For all experiments (heat and salt), the means of three experiments were determined from three independent transformants.…”

Section: Methodsmentioning

confidence: 99%

“…However, LEA family proteins have thus far only been investigated in a few plant species, such as Arabidopsis , rice, poplar, Chinese plum, legume, tomato, potato, barley and Pinus 910111213141516. In contrast, very few LEA genes have been identified in Orchidaceae, and studies on LEA family evolutionary relationships and functional characteristics are scarce.…”

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confidence: 99%

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Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Ling

Guo

2016

Sci Rep

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Late embryogenesis abundant (LEA) proteins, a diverse family, accumulate during seed desiccation in the later stages of embryogenesis. LEA proteins are associated with tolerance to abiotic stresses, such as drought, salinity and high or cold temperature. Here, we report the first comprehensive survey of the LEA gene family in Dendrobium officinale, an important and widely grown medicinal orchid in China. Based on phylogenetic relationships with the complete set of Arabidopsis and Oryza LEA proteins, 17 genes encoding D. officinale LEAs (DofLEAs) were identified and their deduced proteins were classified into seven groups. The motif composition of these deduced proteins was correlated with the gene structure found in each LEA group. Our results reveal the DofLEA genes are widely distributed and expressed in tissues. Additionally, 11 genes from different groups were introduced into Escherichia coli to assess the functions of DofLEAs. Expression of 6 and 7 DofLEAs in E. coli improved growth performance compared with the control under salt and heat stress, respectively. Based on qPCR data, all of these genes were up-regulated in various tissues following exposure to salt and heat stresses. Our results suggest that DofLEAs play an important role in responses to abiotic stress.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Ling

Guo

2016

Sci Rep

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“…An analysis of genes involved in water and cold stress revealed some substantial differences between conifers and Gnetum. The late embryogenesis abundant protein (LEA) gene family encodes crucial proteins that are involved in protecting plants from desiccation or osmotic stresses associated with low temperature 42,43 . An analysis of LEA family members suggests that some members have been reduced in number in Gnetum or expanded in conifers (for example, LEA-3), or lost completely in Gnetum (LEA-4, 5, 6).…”

Section: Water Stressmentioning

confidence: 99%

A genome for gnetophytes and early evolution of seed plants

et al. 2018

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Gnetophytes are an enigmatic gymnosperm lineage comprising three genera, Gnetum, Welwitschia and Ephedra, which are morphologically distinct from all other seed plants. Their distinctiveness has triggered much debate as to their origin, evolution and phylogenetic placement among seed plants. To increase our understanding of the evolution of gnetophytes, and their relation to other seed plants, we report here a high-quality draft genome sequence for Gnetum montanum, the first for any gnetophyte. By using a novel genome assembly strategy to deal with high levels of heterozygosity, we assembled >4 Gb of sequence encoding 27,491 protein-coding genes. Comparative analysis of the G. montanum genome with other gymnosperm genomes unveiled some remarkable and distinctive genomic features, such as a diverse assemblage of retrotransposons with evidence for elevated frequencies of elimination rather than accumulation, considerable differences in intron architecture, including both length distribution and proportions of (retro) transposon elements, and distinctive patterns of proliferation of functional protein domains. Furthermore, a few gene families showed Gnetum-specific copy number expansions (for example, cellulose synthase) or contractions (for example, Late Embryogenesis Abundant protein), which could be connected with Gnetum's distinctive morphological innovations associated with their adaptation to warm, mesic environments. Overall, the G. montanum genome enables a better resolution of ancestral genomic features within seed plants, and the identification of genomic characters that distinguish Gnetum from other gymnosperms. NATuRe PLANTS ArticlesNATurE PLANTs phylogenetic position of gnetophytes, with topologies differing depending on the type of sequence data (for example, plastid versus nuclear genes, nucleotide versus amino acid data) and analytical approach used (for example, maximum parsimony, maximum likelihood, Bayesian, multispecies coalescent based methods) [6][7][8] . Consequently, several possible hypotheses have been put forward that place gnetophytes as sister to (1) Pinaceae ('Gnepine' hypothesis); (2) cupressophytes ('Gnecup' hypothesis); (3) all conifers ('Gnetifer' hypothesis); (4) all other gymnosperms; or (5) all seed plants 9 . Currently, the emerging consensus, based on both older and more recent studies, and recently released data from the 1KP initiative (see https://sites.google.com/a/ualberta.ca/onekp/, and Wickett et al. 8 ), indicates that gnetophytes are sister to, or within, the conifers.So far, the availability of whole genome sequences for gymnosperms has been limited to conifers (specifically to Pinaceae) [10][11][12][13] and G. biloba 14 , with no whole genome assemblies available for the two remaining major seed plant lineages-cycads and gnetophytes. This deficiency, together with the conflicting phylogenetic evidence for relationships among these groups, is impeding our understanding of genome evolution across all seed plants. Here, we present a high-quality draft genome of Gnetum ...

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Insights on the persistence of pines (Pinusspecies) in the Late Cretaceous and their increasing dominance in the Anthropocene

Singh¹,

Inderjit

Singh

et al. 2018

Ecology and Evolution

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Although gymnosperms were nearly swept away by the rise of the angiosperms in the Late Cretaceous, conifers, and pines (Pinus species) in particular, survived and regained their dominance in some habitats. Diversification of pines into fire‐avoiding (subgenus Haploxylon) and fire‐adapted (subgenus Diploxylon) species occurred in response to abiotic and biotic factors in the Late Cretaceous such as competition with emerging angiosperms and changing fire regimes. Adaptations/traits that evolved in response to angiosperm‐fuelled fire regimes and stressful environments in the Late Cretaceous were key to pine success and are also contributing to a new “pine rise” in some areas in the Anthropocene. Human‐mediated activities exert both positive and negative impacts of range size and expansion and invasions of pines. Large‐scale afforestation with pines, human‐mediated changes to fire regimes, and other ecosystem processes are other contributing factors. We discuss traits that evolved in response to angiosperm‐mediated fires and stressful environments in the Cretaceous and that continue to contribute to pine persistence and dominance and the numerous ways in which human activities favor pines.

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Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli

Cited by 90 publications

References 59 publications

Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

A genome for gnetophytes and early evolution of seed plants

Insights on the persistence of pines (Pinusspecies) in the Late Cretaceous and their increasing dominance in the Anthropocene

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