Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1)

Belaaloui, Ghania; Imbert, Anne-Marie; Bardin, Florence; Tonnelle, Cécile; Dubreuil, Patrice; Lopez, Marc; Chabannon, Christian

doi:10.1038/sj.leu.2402916

Cited by 7 publications

(7 citation statements)

References 48 publications

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“…Virus titers decreased by 4 h and all virus was cleared by 7 d p.i. The data indicate that HSV infects CD34 þ cells, which is consistent with recent findings that they express the virus receptor nectin 1 (Belaaloui et al, 2003). CD34 þ cells, however, do not sustain HSV replication and are not latently infected with whole virus that is capable of reactivation.…”

Section: Resultssupporting

confidence: 90%

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM)

Ono

Sharma

Smith

et al. 2005

Journal of Investigative Dermatology

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Herpes simplex virus (HSV)-associated erythema multiforme (HAEM) is a recurrent disease characterized by the presence and expression of HSV DNA fragments in lesional skin. Our studies examined the mechanism of viral DNA transport to the skin of HAEM patients. CD34+ cells were isolated from the blood of normal subjects and HSV and HAEM patients during acute lesions and at quiescence. They were cultured with cytokines that favor their differentiation into Langerhans cells (LC) precursors (CD1a+/CD14-) and examined for HSV replication, HSV-induced cellular alterations, viral DNA fragmentation, and clearance. CD34+ cells from all study groups were non-permissive for HSV replication but infection favored their differentiation into CD1a+/CD14- LC precursors and upregulated E-cadherin expression, thereby assisting LC targeting to the skin. Only HAEM patients had CD34+ cells that retained viral DNA fragments, notably polymerase DNA, for at least 7 d of in vitro culture. The percentages of circulating CD34+ (and CD34+/CLA+) cells were significantly higher in HAEM patients at the time of acute lesions. A similar increase was not seen for HSV patients. The data are the first report implicating CD34+ cells in HAEM pathogenesis, likely by transporting HSV DNA fragments to lesional skin.

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Section: Resultssupporting

confidence: 90%

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM)

Ono

Sharma

Smith

et al. 2005

Journal of Investigative Dermatology

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“…Therefore, it is not likely that the different fates of cells during differentiation are responsible for heterogeneity in SSEA-1 expression. Another possible explanation is that SSEA-1 antigen is expressed differently during different phases of the cell cycle, as occurs for other anti- gens (Belaaloui et al 2003). More work is necessary to elucidate the significance of SSEA-1 heterogeneity.…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation

Cui

Johkura

Yue

et al. 2004

J Histochem Cytochem.

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S U M M A R YWe examined the distribution of cell adhesion-related molecules (CAMs) among mouse embryonic stem (ES) cells and the spatial distribution on cell surfaces before and during differentiation. The cell-cell heterogeneity of SSEA-1, PECAM-1, and ICAM-1 among the undifferentiated cells in the ES cell colonies was evident by immunohistochemistry and immuno-SEM, supporting the flow cytometry findings. In contrast, most undifferentiated ES cells strongly expressed CD9. SSEA-1 was located preferentially on the edge of low protuberances and microvilli and formed clusters or linear arrays of 3-20 particles. PECAM-1 and ICAM-1 were randomly localized on the free cell surfaces, whereas CD9 was preferentially localized on the microvilli or protuberances, especially in the cell periphery. Both the SSEA-1 ϩ fraction and the SSEA-1 Ϫ fraction of magnetic cell sorting (MACS) formed undifferentiated colonies after plating. Flow cytometry showed that these populations reverted separately again to a culture with a mixed phenotype. Differentiation induced by retinoic acid downregulated the expression of all CAMs. Immuno-SEM showed decreases of SSEA-1 in the differentiated ES cells, although some clustering still remained. Our findings help to elucidate the significance of these molecules in ES cell maintenance and differentiation and suggest that cell surface antigens may be useful for defining the phenotype of undifferentiated and differentiated ES cells.

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“…An equal volume of a 50-g/mL propidium iodide solution was added, and cells were incubated for 1 hour at 37°C before flow cytometry analysis. 46 Detection of apoptotic cells. Apoptosis was determined by monitoring phosphatidyl serine exposure at the outer cell membrane, using an assay based on binding of annexin V-Cy5 (Molecular Probes); Sytox was used as a viability probe.…”

Section: Limiting Dilution Analysis Of Cobblestone Area Colony-forminmentioning

confidence: 99%

“…CAFCs were scored at week 5. 45,46 Erythroid cell differentiation assay. The technique was adapted from a previously published report.…”

Section: Limiting Dilution Analysis Of Cobblestone Area Colony-forminmentioning

confidence: 99%

CD99 expressed on human mobilized peripheral blood CD34+ cells is involved in transendothelial migration

Imbert

Belaaloui

Bardin

et al. 2006

Blood

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Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1)

Cited by 7 publications

References 48 publications

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM)

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM)

Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation

CD99 expressed on human mobilized peripheral blood CD34+ cells is involved in transendothelial migration

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