Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation

Cui, Li; Johkura, Kohei; Yue, Fengming; Ogiwara, Naoko; Okouchi, Yasumitsu; Asanuma, Kazuhiko; Sasaki, Katsunori

doi:10.1369/jhc.3a6241.2004

Cited by 90 publications

(83 citation statements)

References 34 publications

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“…The different expression of CD9, nanog and taube nuss that we observed in adult myocardium could be associated to the degree of differentiation of embryonic stem cells. Indeed, it has been reported that nanog and CD9 expression was elevated in undifferentiated embryonic stem cells and down-regulated during their differentiation, in concomitance with the loss of the pluripotency (Cui et al, 2004;Hart et al, 2004). Since it has been already demonstrated that the absence of taube nuss causes apoptosis of stem cells from blastocisty inner cellular mass (Voss et al, 2000), we postulate that this factor could be important for stem cell survival even in adult heart.…”

Section: Discussionmentioning

confidence: 71%

Research of cardiomyocyte precursors in adult rat heart

Bellafiore

Cappello

et al. 2006

Tissue and Cell

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Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability.

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Section: Discussionmentioning

confidence: 71%

Research of cardiomyocyte precursors in adult rat heart

Bellafiore

Cappello

et al. 2006

Tissue and Cell

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“…Recent reports have demonstrated that surface markers, such as Ssea1, Pecam1, and Icam1, are expressed heterogeneously in mouse ES cell cultures, and their expression patterns appear to be modulated by differentiation processes in these cultures (Cui et al, 2004;Li et al, 2005). Furthermore, in the case of Pecam1, overall expression levels and isoform distributions are indicative of ES cell differentiation state (Cui et al, 2004;Li et al, 2005) as well as embryonic developmental potential (Furusawa et al, 2004).…”

Section: Rationale For the Studymentioning

confidence: 99%

An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells

Carter

Stagg

Falco

et al. 2008

Gene Expression Patterns

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We previously reported that Zscan4 showed heterogeneous expression patterns in mouse embryonic stem (ES) cells. To identify genes that show similar expression patterns, we carried out highthroughput in situ hybridization assays on ES cell cultures for 244 genes. Most of the genes are involved in transcriptional regulation, and were selected using microarray-based comparisons of gene expression profiles in ES and embryonal carcinoma (EC) cells versus differentiated cell types. Pou5f1 (Oct4, Oct3/4) and Krt8 (EndoA) were used as controls. Hybridization signals were detected on ES cell colonies for 147 genes (60%). The majority (136 genes) of them showed relatively homogeneous expression in ES cell colonies. However, we found that two genes unequivocally showed Zscan4-like spotted expression pattern (spot-in-colony pattern; Whsc2 and Rhox9). We also found that nine genes showed relatively heterogeneous expression pattern Rest, Atf4, Pa2g4, E2f2, Nanog, Dppa3/Pgc7/Stella, Esrrb, and Fscn1). Among these genes, Zfp42/Rex1 showed unequivocally heterogeneous expression in individual ES cells prepared by the CytoSpin. These results show the presence of different types or states of cells within ES cell cultures otherwise thought to be undifferentiated and homogeneous, suggesting a previously unappreciated complexity in ES cell cultures. KeywordsES cells; EC cells; pluripotent stem cells; heterogeneous gene expression; homogeneous gene expression; Zscan4; Pou5f1; Oct4; Oct3/4; Krt8; EndoA; Whsc2; Nelfa; Rhox9; Zfp42; Rex1; Rest; Zfp42; Rex1; Rest; Atf4; Pa2g4; E2f2; Nanog; Dppa3; Pgc7; Stella; Esrrb; Fscn1 &Corresponding author: Minoru S.H. Ko, M.D., Ph.D., Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, Triad Technology Center, suite 3000, 333 Cassell Drive, Baltimore, MD 21224, USA, Phone: (410) 558-8359; Fax: (410) 558-8331, E-mail: kom@mail.nih.gov. # Current Address: Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut, 06269 $ Current Address: Stem Cell and Drug Discovery Institute, Kyoto 600-8813, Japan @ Current Address: Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan * Equal contribution Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. (Falco et al., 2007). We suspect the existence of other transcription-regulating genes that show similar expression patterns. NIH Public AccessThe goals of this study are to find additional evidence for heterogeneous cell populations within undifferentiated ES cell cultures by identifying such genes and characterizing their expression patte...

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“…Furthermore, they require less LIF, probably due to the homogeneous exposure of cells to this cytokine (figures 6(b) and (c)). It has also been reported that cell-cell adhesion molecules in undifferentiated ES cell colonies can be distributed heterogeneously [37]. These observations indicate that an aggregated colony formation with close cell-cell communications may generate a heterogeneous environment within the colonies, which potentially inhibit the proliferation of ES cells and the distribution of soluble factors.…”

Section: Single Cell Culture On Matricesmentioning

confidence: 90%

Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials

Haque

Nagaoka

Hexig

et al. 2010

Science and Technology of Advanced Materials

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Nanobiomaterials can play a central role in regenerative medicine and tissue engineering by facilitating cellular behavior and function, such as those where extracellular matrices (ECMs) direct embryonic stem (ES) cell morphogenesis, proliferation, differentiation and apoptosis. However, controlling ES cell proliferation and differentiation using matrices from natural sources is still challenging due to complex and heterogeneous culture conditions. Moreover, the systemic investigation of the regulation of self-renewal and differentiation to lineage specific cells depends on the use of defined and stress-free culture conditions. Both goals can be achieved by the development of biomaterial design targeting ECM or growth factors for ES cell culture. This targeted application will benefit from expansion of ES cells for transplantation, as well as the production of a specific differentiated cell type either by controlling the differentiation in a very specific pathway or by elimination of undesirable cell types.

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Spatial Distribution and Initial Changes of SSEA-1 and Other Cell Adhesion-related Molecules on Mouse Embryonic Stem Cells Before and During Differentiation

Cited by 90 publications

References 34 publications

Research of cardiomyocyte precursors in adult rat heart

Research of cardiomyocyte precursors in adult rat heart

An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells

Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials

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