Functional Characterization of Chemosensory Proteins in the Scarab Beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeida)

Sun, Hongyan; Guan, Li; Feng, Honglin; Yin, Jing; Cao, Yujin; Xi, Jinghui; Li, Kebin

doi:10.1371/journal.pone.0107059

Cited by 22 publications

(18 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According Larsson et al (2001) study of A. cuprea, the placoid sensilla of peripheral area of lamellae are responsible for detecting sexual pheromones, while sensilla in the central part detect plant volatiles and other odors. Otherwise, Sun et al (2014) suggested that sensilla placodea was plant volatile main receptor and coeloconica as sexual attractant receptor to Holotrichia oblita (Faldermann, 1835) (Melolonthinae).…”

Section: Discussionmentioning

confidence: 99%

Aspects of mating behavior and antennal sensilla in Anomala inconstans Burmeister, 1844 (Coleoptera: Scarabaeidae: Rutelinae)

2019

View full text Add to dashboard Cite

When suitable, adults of Scarabaeidae usually form swarms to find food and breeding sites. The steps of mating behavior can be mediated by chemical communication, and antennal sensilla are released volatiles detection structures, as sexual pheromones. In present work the mating behavior and the antennal sensilla of Anomala inconstans Burmeister, 1844 are described. The study was conducted at the Universidade Estadual de Mato Grosso do Sul, Cassilândia, Brazil from March 2015 to December 2017. Adults were collected through a light trap and taken to the laboratory for studies. Field and laboratory observations provided data for the description of the steps of mating behavior. Adults swarms were registered from September to November 2015 at 05:30 pm to 00:00 am. Females display a calling behavior from 05:25 pm to 08:00 pm, in which they rub their posterior legs against their abdomen, and after a few minutes males are able to locate them. In laboratory, the mating process lasted 20.4 minutes on average, and the possibility of chemical communication between adults was here discussed. The antennae of the species have trichoid, chaetica, placoid types I, II and III, and coeloconic types I and II sensilla. Placoid sensilla are the most abundant and females have more sensilla than males.

show abstract

Section: Discussionmentioning

confidence: 99%

Aspects of mating behavior and antennal sensilla in Anomala inconstans Burmeister, 1844 (Coleoptera: Scarabaeidae: Rutelinae)

2019

View full text Add to dashboard Cite

show abstract

“…In olfactory perception, CSPs have similar functions to OBP. The hydrophobic pocket of CSPs can also recognize and transport chemical signals to chemoreceptors (Sun et al, 2014 ; Wang et al, 2016 ). Our results show that BodoCSP3/5 were antennae-enriched and might be involved in the chemosensory process.…”

Section: Discussionmentioning

confidence: 99%

Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in Bradysia odoriphaga (Diptera: Sciaridae)

et al. 2018

View full text Add to dashboard Cite

Bradysia odoriphaga is an agricultural pest insect affecting the production of Chinese chive and other liliaceous vegetables in China, and it is significantly attracted by sex pheromones and the volatiles derived from host plants. Despite verification of this chemosensory behavior, however, it is still unknown how B. odoriphaga recognizes these volatile compounds on the molecular level. Many of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play crucial roles in olfactory perception. Here, we identified 49 OBP and 5 CSP genes from the antennae and body transcriptomes of female and male adults of B. odoriphaga, respectively. Sequence alignment and phylogenetic analysis among Dipteran OBPs and CSPs were analyzed. The sex- and tissue-specific expression profiles of 54 putative chemosensory genes among different tissues were investigated by quantitative real-time PCR (qRT-PCR). qRT-PCR analysis results suggested that 22 OBP and 3 CSP genes were enriched in the antennae, indicating they might be essential for detection of general odorants and pheromones. Among these antennae-enriched genes, nine OBPs (BodoOBP2/4/6/8/12/13/20/28/33) were enriched in the male antennae and may play crucial roles in the detection of sex pheromones. Moreover, some OBP and CSP genes were enriched in non-antennae tissues, such as in the legs (BodoOBP3/9/19/21/34/35/38/39/45 and BodoCSP1), wings (BodoOBP17/30/32/37/44), abdomens and thoraxes (BodoOBP29/36), and heads (BodoOBP14/23/31 and BodoCSP2), suggesting that these genes might be involved in olfactory, gustatory, or other physiological processes. Our findings provide a starting point to facilitate functional research of these chemosensory genes in B. odoriphaga at the molecular level.

show abstract

“…However, several pieces of evidence now indicate strongly that at least some members of the CSP family are involved in chemodetection and should be regarded as a second class of binding proteins. In particular: ( i ) CSPs are abundant in the lymph of chemosensory hairs, both in olfactory and contact sensilla, in locusts (Angeli et al., ; Jin et al., ), phasmids (Monteforti et al., ), Lepidoptera (Jacquin‐Joly et al., ) and Coleoptera (Sun et al., ); ( ii ) they bind semiochemicals with micromolar dissociation constants, similarly to OBPs (Iovinella et al., ). In particular, CSP3 of the honeybee [reported in the original work as antennal specific protein 3 (ASP3)], specifically binds some components of brood pheromone (Briand et al., ); ( iii ) in some species, such as the paper wasp Polistes dominulus (Calvello et al., ) and the Argentine ant Linepithema humile (Ishida, Chiang & Leal, ), some CSPs seem to be exclusive to or most abundant in the antennae.…”

Section: Multiple Functions Of Obps and Cspsmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

View full text Add to dashboard Cite

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.

show abstract

Functional Characterization of Chemosensory Proteins in the Scarab Beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeida)

Cited by 22 publications

References 62 publications

Aspects of mating behavior and antennal sensilla in Anomala inconstans Burmeister, 1844 (Coleoptera: Scarabaeidae: Rutelinae)

Aspects of mating behavior and antennal sensilla in Anomala inconstans Burmeister, 1844 (Coleoptera: Scarabaeidae: Rutelinae)

Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in Bradysia odoriphaga (Diptera: Sciaridae)

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

Contact Info

Product

Resources

About