Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in Bradysia odoriphaga (Diptera: Sciaridae)

Abstract

Bradysia odoriphaga is an agricultural pest insect affecting the production of Chinese chive and other liliaceous vegetables in China, and it is significantly attracted by sex pheromones and the volatiles derived from host plants. Despite verification of this chemosensory behavior, however, it is still unknown how B. odoriphaga recognizes these volatile compounds on the molecular level. Many of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play crucial roles in olfactory perception. Here, we identified 49 OBP and 5 CSP genes from the antennae and body transcriptomes of female and male adults of B. odoriphaga, respectively. Sequence alignment and phylogenetic analysis among Dipteran OBPs and CSPs were analyzed. The sex- and tissue-specific expression profiles of 54 putative chemosensory genes among different tissues were investigated by quantitative real-time PCR (qRT-PCR). qRT-PCR analysis results suggested that 22 OBP and 3 CSP genes were enriched in the antennae, indicating they might be essential for detection of general odorants and pheromones. Among these antennae-enriched genes, nine OBPs (BodoOBP2/4/6/8/12/13/20/28/33) were enriched in the male antennae and may play crucial roles in the detection of sex pheromones. Moreover, some OBP and CSP genes were enriched in non-antennae tissues, such as in the legs (BodoOBP3/9/19/21/34/35/38/39/45 and BodoCSP1), wings (BodoOBP17/30/32/37/44), abdomens and thoraxes (BodoOBP29/36), and heads (BodoOBP14/23/31 and BodoCSP2), suggesting that these genes might be involved in olfactory, gustatory, or other physiological processes. Our findings provide a starting point to facilitate functional research of these chemosensory genes in B. odoriphaga at the molecular level.

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

“…The main host plant of B. odoriphaga is Chinese chive ( Allium tuberosum Rottle ex Spreng), a perennial vegetable that originated in China and is widely cultivated in East Asia . Bradysia odoriphaga larvae usually accumulate in the roots, bulbs and even immature stems of Chinese chives, making the pest hard to control, leading to significant production losses of Chinese chives …”

“…[19][20][21] Bradysia odoriphaga larvae usually accumulate in the roots, bulbs and even immature stems of Chinese chives, making the pest hard to control, leading to significant production losses of Chinese chives. 22,23 Neonicotinoid insecticides act on the insect nicotinic acetylcholine receptor, and possess high control activity against sucking pests and some dipteran, coleopteran and lepidopteran pest species. [24][25][26] Because B. odoriphaga larvae usually accumulate in the bulbs of Chinese chive plants, neonicotinoid insecticides such as imidacloprid and thiamethoxam have good field control efficacy towards B. odoriphaga due to their internal conduction within the plant.…”

Selection of Reference Genes for Quantitative Real-Time PCR in Aquatica leii (Coleoptera: Lampyridae) Under Five Different Experimental Conditions

“…Chemosensory proteins, including olfaction-related genes in insect antennae, play important roles in olfaction ( Zhang L. W. et al, 2016 ; Wang et al, 2017 ). Previous studies have reported the antenna-specific expression of CSPs in insects ( Zhao et al, 2018 ). To confirm the reliability of the selected RGs, the expression profiles of the target gene CYP3A and CSP8 were determined in different concentrations of BaP and adult tissues, respectively, and independently normalized with the two most stable RGs as well as the least stable RGs.…”

Characterization of MaltOBP1, a Minus-C Odorant-Binding Protein, From the Japanese Pine Sawyer Beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae)

“…Studies involving OBP identification (Li L. et al, 2017;Zhao et al, 2018), protein structural analysis (Northey et al, 2016;Falchetto et al, 2019), immunocytochemistry in specific olfactory sensilla Zhang et al, 2018), ligand binding properties (Sun et al, 2017(Sun et al, , 2018, and in vivo RNA interference (Yin et al, 2018;He et al, 2019) have demonstrated that insect OBPs play crucial roles in odorant discrimination, binding, and transportation. These proteins carry lipophilic odorants to the olfactory receptor cells in the sensillar lymph surrounding the sensory dendrite (Pelosi et al, 2014(Pelosi et al, , 2018.…”