Functional Characterization of a New Class of Odorant-Binding Proteins in the MothMamestra brassicae

Bohbot, Jonathan D.; Sobrio, Franck; Lucas, Philippe; Meillour, Patricia Nagnan‐Le

doi:10.1006/bbrc.1998.9806

Cited by 71 publications

(62 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it is not a six-cysteine odorant-binding protein, but a four-cysteine chemosensory protein. Although the physiological role of CSPs has not been clarified to date, their demonstrated ability to bind several ligands (Ban et al 2002;Bohbot et al 1998;Jacquin-Joly et al 2001;Nagnan-Le Meillour et al 2000;Lartigue et al 2002) suggests that they may have a function analogous to that of pheromone-binding proteins. In ants, the perireceptor events (Kaissling 2001) may involve CSPs in the transport of ligands to the receptors.…”

Section: Resultsmentioning

confidence: 99%

“…A third class of small proteins has been identified in several sensorial organs from a number of insect orders and classified as chemosensory proteins (CSPs). These proteins contain only four cysteine residues and share no sequence "homology" with OBPs, although they also may function as carrier proteins (Bohbot et al 1998;Campanacci et al 2001;Jacquin-Joly et al 2001;Marchese et al 2000;Lartigue et al 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protein that makes sense in the Argentine ant

Ishida

Chiang

Leal

2002

Naturwissenschaften

View full text Add to dashboard Cite

With a protein-based approach, we have identified and cloned the cDNA encoding a chemosensory protein (LhumCSP) in the Argentine ant, Linepithema humile. The open reading frame of the cloned cDNA encoded a signal peptide (20 residues), and a mature protein (pI 4.62) of 106 amino acid residues. The calculated molecular mass (12,453 Da) was in agreement with the molecular mass measured by on-line chromatography-electrospray ionization mass spectrometry (12,448 Da), given the formation of two disulfide bridges. LhumCSP shared sequence similarity with various CSPs, particularly those identified and/or cloned from moth species. Also, LhumCSP showed the hallmark of the chemosensory proteins, i.e., four well conserved cysteine residues. The antennal protein was not detected in non-olfactory tissues (leg and thorax) contrary to a putative pheromone-binding protein isolated from the thorax of the red imported fire ant, Solenopsis invicta. In addition, these findings suggest that, as in Orthopterans and Phasmids, the protein that makes sense in the Argentine ant is not an odorant-binding protein, but rather a chemosensory protein.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein that makes sense in the Argentine ant

Ishida

Chiang

Leal

2002

Naturwissenschaften

View full text Add to dashboard Cite

show abstract

“…This hypothesis is based on three facts: their specific localization to the sensillum lymph Nagnan-Le Meillour et al, 2000;Monteforti et al, 2002), the globular 3-D structure with six a-helices surrounding an internal hydrophobic binding channel, and the capability of binding a variety of chemicals (Bohbot et al, 1998;Nagnan-Le Meillour et al, 2000;Jacquin-Joly et al, 2001;Briand et al, 2002;Lartigue et al, 2002;Campanacci et al, 2003, Mosbah et al, 2003. Nevertheless, insect CSP genes are also expressed in other tissues including the legs, head, thorax, wings, labial palps, tarsi, proboscis, pheromone glands and ejaculatory ducts.…”

Section: Discussionmentioning

confidence: 99%

“…They are also known as sensory appendage proteins (SAPs; Robertson et al, 1999). So far, several members of this class of protein have been described in insects of different orders, including Lepidoptera (Maleszka and Stange, 1997;Bohbot et al, 1998;Nagnan-Le Meillour et al, 2000;Picimbon et al, 2000aPicimbon et al, , 2001Jacquin-Joly et al, 2001;Robertson et al, 1999), Orthoptera Picimbon et al, 2000b;Ban et al, 2003), Hymenoptera (Danty et al, 1998;Briand et al, 2002;Ishida et al, 2002), Blattoidea (Kitabayashi et al, 1998;Picimbon and Leal, 1999), Phasmatodea Mameli et al, 1996;Marchese et al, 2000), and Hemiptera (Jacobs et al, 2005). Zhou et al (2006) identified 74 genes encoding putative CSPs of insect species from 10 different orders based on genome and expressed sequence tags (EST).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori

Gong¹,

Zhang²,

Zhao³

et al. 2007

Insect Biochemistry and Molecular Biology

161

173

View full text Add to dashboard Cite

“…However, the exact physiological role of CSPs has still to be identified. In the moth Mamestra brassicae, several CSPs have been identified in the proboscis (12) and in the antennae (13). M. brassicae CSPs have been shown to bind several components of the pheromonal blend and therefore might also have a function analogous to that of PBPs (12).…”

mentioning

confidence: 99%

X-ray Structure and Ligand Binding Study of a Moth Chemosensory Protein

Lartigue¹,

Campanacci²,

Roussel³

et al. 2002

Journal of Biological Chemistry

161

187

View full text Add to dashboard Cite

Two classes of highly soluble and very abundant proteins of ϳ150 amino acids have been detected in sensilla of Lepidoptera, both containing 6 conserved cysteines forming three disulfide bridges. The first class, that of GOBPs, 1 is equally distributed in both sexes, whereas the second class, that of PBPs, is mainly present in males (1). A third class of small proteins (average M r 13,000) has been identified in antennae from Drosophila melanogaster and in antennae and several sensorial organs (tarsi, labrum) from a wide range of species of the insect order (2-11). These proteins have been proposed to be involved in CO 2 detection (3), in chemical signal transmission in regenerating legs (5), or in chemo-perception (either olfaction or taste (9, 12)), and they were therefore called chemosensory proteins (CSPs). CSPs are shorter (110 -115 amino acids) than PBP or GOBP, contain only 4 conserved cysteines forming two disulfide bridges (9), and share no sequence homology with them.

show abstract

Functional Characterization of a New Class of Odorant-Binding Proteins in the MothMamestra brassicae

Cited by 71 publications

References 32 publications

Protein that makes sense in the Argentine ant

Protein that makes sense in the Argentine ant

Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori

X-ray Structure and Ligand Binding Study of a Moth Chemosensory Protein

Contact Info

Product

Resources

About