Functional Characterization of a Miniature Inverted Transposable Element at the Origin of
            <i>mcr-5</i>
            Gene Acquisition in Escherichia coli

Kieffer, Nicolas; Nordmann, Patrice; Millemann, Yves; Poirel, Laurent

doi:10.1128/aac.00559-19

Cited by 16 publications

(16 citation statements)

References 29 publications

(33 reference statements)

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“…While in transposon Tn6952, the IRR shared 67.5% nucleotide identity with the IRL. The TnAS3 (Kieffer et al, 2019) and TnAS1 (Liu et al, 2017) transposases could mobilize the resistance genes mcr-3 and mcr-5 by IRs, respectively. So, we speculate that the two IRs and transposase of TnAS3 and TnAS1 may be involved in the molecular transposition of plasmid pS13-4 through forming an efficient mobilization unit Tn6952.…”

Section: Proposed Formation Mechanism Of Two Fusion Plasmidsmentioning

confidence: 99%

The Formation of Two Hybrid Plasmids Mediated by IS26 and Tn6952 in Salmonella enterica Serotype Enteritidis

Liu

Zhang

et al. 2021

Front. Microbiol.

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To characterize the formation mechanism and characteristics of two cointegrate plasmids in Salmonella enterica serotype Enteritidis strain S13, plasmids from strain S13 and three corresponding transconjugants were subjected to whole genome sequencing and analyzed using bioinformatics tools. The traits of two fusion plasmids in transconjugants were characterized by stability and conjugation experiments. Sequence analysis indicated that strain S13 contained four plasmids, including mcr-1-bearing pS13-1, blaCTX–M–55-carrying pS13-2, tet(M)-bearing pS13-3, and floR-carrying pS13-4. IncN1-F33:A–:B– plasmid pS13-2, respectively, fused with IncFI:A–:B– plasmid pS13-3 and IncX1 plasmid pS13-4, which generated two cointegrate plasmids, designated pS13D and pS13F, which involved in two intermolecular replicative mechanisms mediated by IS26 and the novel transposon Tn6952 (ΔTnAS3-IS26-ΔISEcp1-ramA-ΔIS26-ΔTnAS1), respectively. This is the first report of the fusion of the IncN1-F33:A–:B– plasmid and IncFI:A–:B– plasmid mediated by IS26, and with IncX1 plasmid mediated by Tn6952. The formation and evolution of cointegrate plasmids could expand the resistance and host spectrum of fusion plasmids.

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Section: Proposed Formation Mechanism Of Two Fusion Plasmidsmentioning

confidence: 99%

The Formation of Two Hybrid Plasmids Mediated by IS26 and Tn6952 in Salmonella enterica Serotype Enteritidis

Liu

Zhang

et al. 2021

Front. Microbiol.

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“…Plasmids conferring antibiotic resistance are known to be stable in the environment, even without antibiotic selection pressures [13,15,17,58,59]. Especially, the presence of self-transmissible plasmids, such as the atrazine catabolic plasmid pADP-1 [60], and putative conjugative plasmids, such as pAR060302 [61] and pPS1 [62], may reinforce the hypothesis of frequent plasmid transfer events between ARGs and bacterial communities during treatment processes, or these plasmids may spread through the acceleration of the ARGs mobility. Thus, the high abundance of plasmids potentially leads to the enrichment of multiple ARGs in wastewater treatment processes.…”

Section: Prevalence Of Mobile Genetic Elements (Mges)mentioning

confidence: 99%

Metagenomic Profiles of Antibiotic Resistance Genes in Activated Sludge, Dewatered Sludge and Bioaerosols

Han

Yoo

2020

Water

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Wastewater treatment plants (WWTPs) have been considered hotspots for the development and dissemination of antibiotic resistance in the environment. Although researchers have reported a significant increase in bioaerosols in WWTPs, the associated bacterial taxa, antibiotic resistance genes (ARGs), and mobile genetic elements (MGEs) remain relatively unknown. In this study, we have investigated the abundance and occurrences of ARGs and MGEs, as well as the bacterial community compositions in activated sludge (AS), dewatered sludge (DS) and bioaerosols (BA) in a WWTP. In total, 153 ARG subtypes belonging to 19 ARG types were identified by the broad scanning of metagenomic profiles obtained using Illumina HiSeq. The results indicated that the total occurrences and abundances of ARGs in AS and DS samples were significantly higher than those in BA samples (p < 0.05). However, some specific ARG types related to sulfonamide, tetracycline, macrolide resistance were present in relatively high abundance in BA samples. Similar to many other full-scale WWTPs, the Proteobacteria (58%) and Bacteroidetes (18%) phyla were dominant in the AS and DS samples, while the Firmicutes (25%) and Actinobacteria (20%) phyla were the most dominant in the BA samples. Although the abundance of genes related to plasmids and integrons in bioaerosols were two to five times less than those in AS and DS samples, different types of MGEs were observed in BA samples. These results suggest that comprehensive analyses of resistomes in BA are required to better understand the emergence of both ARGs and MGEs in the wastewater treatment process due to the significant increase of scientific attention toward bioaerosols effects.

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“…Enzymatic pathways are imposed by the expression of antibiotic inactivating enzymes, while nonenzymatic pathways develop mainly because of gene mutations which result in resistance due to changes in e ux pumps, membrane permeability or target molecules [8]. Genes encoding resistance enzymes can be derived either from the bacterium itself or from Miniature Inverted Transposable Elements (MITEs) such as the plasmid encoding beta-lactamases or aminoglycoside-modifying enzymes [9,10]. The presence of genes encoding beta-lactamase and carbapenems enzymes are the most important mechanisms of antibiotic resistance in K. pneumoniae [11].…”

Section: Introductionmentioning

confidence: 99%

Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae

Ahmadi

Ranjbar

Behzadi

et al. 2021

Expert Review of Anti-infective Therapy

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Background Klebsiella pneumoniae is armed with a wide range of antibiotic resistance mechanisms which mostly challenges effective treatment. Due to this fact, the aims of the current study were to identify the clinical strains of K . pneumoniae as well as to determine their phenotypes and molecular characterization related to antimicrobial resistance and virulence genes. Methods In this investigation, specimens from a hospital and different laboratories located in Shahr-e-Qods, Tehran, Iran were collected during a period of nine-month (December 2018 to August 2019). The isolated strains of K. pneumoniae were then identi ed through standard microbial and biochemical assays. Additionally, disk diffusion, combined disk, modi ed Hodge test and PCR were performed for antibiotic resistance of the strains and virulence genes pro ling, respectively. The molecular typing was accomplished by ERIC-PCR. Results Eighty-four isolates of K. pneumoniae were identi ed and subjected to the study. Fifty-two percent of the isolated strains of K. pneumoniae were detected as multidrug resistant (MDR) pathotypes with the highest resistance to ceftriaxone (65%) and the lowest resistance to colistin (23%). Twenty-seven (52%) out of 52 (100%) MDR pathotypes of isolated K. pneumoniae were identi ed as ESBL producers.According to Modi ed Hodge Test (MHT) results, out of 24 resistant strains of isolated K. pneumoniae to imipenem and meropenem, 15 pathotypes (62.5%) were detected as KPC producers. The gene of blaCTX (encoding carbapenemase) with 96% ranked rst, while the blaKPC gene with the prevalence of 71% ranked second among ESBL producers. The aminoglycoside resistance gene of Aac6-Ib showed the highest frequency with the prevalence percentage of 90%. The virulence genes of mrkD (94%) and magA (11%) were the highest and lowest among isolates, respectively. According to ERIC-PCR results the isolated strains of K. pneumoniae were divided into four clusters in which the cluster 4 was predominant group. Conclusions The high prevalence of antibiotic resistance and virulence genes in conjunction with a signi cant relationship between the strains reveals a high pathogenic capacity of the isolated pathotypes of K. pneumoniae . These ndings emphasize the choose of more effective antibiotic regimens for treatment of infections caused by K. pneumoniae.

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Functional Characterization of a Miniature Inverted Transposable Element at the Origin of mcr-5 Gene Acquisition in Escherichia coli

Cited by 16 publications

References 29 publications

The Formation of Two Hybrid Plasmids Mediated by IS26 and Tn6952 in Salmonella enterica Serotype Enteritidis

The Formation of Two Hybrid Plasmids Mediated by IS26 and Tn6952 in Salmonella enterica Serotype Enteritidis

Metagenomic Profiles of Antibiotic Resistance Genes in Activated Sludge, Dewatered Sludge and Bioaerosols

Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae

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