Functional characterization of a mammalian transcription factor, Elongin A

Yasukawa, Takashi; Sugimura, Kazuto; Fukuda, Mizue; Yamazaki, Kanami; Kitajima, Shigetaka; Okumura, Katsuhiko; Aso, Teijiro

doi:10.1016/j.bbrc.2006.11.012

Cited by 8 publications

(16 citation statements)

References 28 publications

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“…Our reporter assays using various Elongin A mutants demonstrated that the C-terminal elongation activation domain including the BC box is crucial for the response of ATF3 gene, consistent with the activity in vitro (22,25,63). In addition, the N-terminal SII similarity region has a mild but positive regulatory role for the Elongin A-driven transcription activity in vivo (Fig.…”

Section: Discussionmentioning

confidence: 68%

“…Mammalian expression plasmids for rat Elongin A deletion mutants were as described (22). Rabbit polyclonal anti-RNAPII (N-20), anti-TFIIE-␣ (C-17), and anti-FCP1 (C-16) antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Within this C-terminal domain is a SOCS box containing the Elongin B and C binding site at residues 550 -588 that together with Elongins B and C is required for maximal transcriptional activity in vitro (25). Recently, we showed that the C-terminal elongation activation domain contains a binding site for RNAPII between residues 590 and 690; this region of Elongin A is crucial for in vitro elongation activity (22).…”

mentioning

confidence: 99%

“…Elongin A inter-acts directly with RNAPII in vitro (22) and carries the elongation stimulatory activity of Elongin (21); at least three isoforms of Elongin A (A, A2, and A3) are present in human (23,24). Elongin A contains an N-terminal region (residues 1-120) similar to the N terminus of SII that is dispensable for its elongation activity and a C-terminal elongation stimulatory domain that falls between residues 400 and 773 (25).…”

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confidence: 99%

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Transcriptional Properties of Mammalian Elongin A and Its Role in Stress Response

Kawauchi

Inoue

Fukuda

et al. 2013

Journal of Biological Chemistry

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Background: Transcriptional elongation is a rate-limiting step in activation of stress response genes. Results: Optimal expression of stress response regulator ATF3 requires the elongation activity but not the ubiquitination activity of Elongin A. Conclusion: Elongin A plays a key role for the adequate expression of ATF3 in vivo. Significance: RNAPII ubiquitination and transcriptional elongation are independent activities of Elongin A.

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Section: Discussionmentioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Transcriptional Properties of Mammalian Elongin A and Its Role in Stress Response

Kawauchi

Inoue

Fukuda

et al. 2013

Journal of Biological Chemistry

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“…Elongin A, also known as transcription elongation factor S-III, increases the rate of transcription by suppressing transient pausing in the elongation complex (Yasukawa et al, 2007). CRSP70 is a subunit of the CRSP (cofactor required for Sp1 activation) complex which is required for the activity of the enhancer binding protein Sp1 Deniaud et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The TFIIS and TFIIS-like genes from Medicago truncatula are involved in oxidative stress response

Macovei

Balestrazzi

Confalonieri

et al. 2011

Gene

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Genotoxic stress and DNA repair in plants: emerging functions and tools for improving crop productivity

et al. 2010

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Crop productivity is strictly related to genome stability, an essential requisite for optimal plant growth/development. Genotoxic agents (e.g., chemical agents, radiations) can cause both chemical and structural damage to DNA. In some cases, they severely affect the integrity of plant genome by inducing base oxidation, which interferes with the basal processes of replication and transcription, eventually leading to cell death. The cell response to oxidative stress includes several DNA repair pathways, which are activated to remove the damaged bases and other lesions. Information concerning DNA repair in plants is still limited, although results from gene profiling and mutant analysis suggest possible differences in repair mechanisms between plants and other eukaryotes. The present review focuses on the base- and nucleotide excision repair (BER, NER) pathways, which operate according to the most common DNA repair rule (excision of damaged bases and replacement by the correct nucleotide), highlighting the most recent findings in plants. An update on DNA repair in organelles, chloroplasts and mitochondria is also provided. Finally, it is generally acknowledged that DNA repair plays a critical role during seed imbibition, preserving seed vigor. Despite this, only a limited number of studies, described here, dedicated to seeds are currently available.

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Functional characterization of a mammalian transcription factor, Elongin A

Cited by 8 publications

References 28 publications

Transcriptional Properties of Mammalian Elongin A and Its Role in Stress Response

Transcriptional Properties of Mammalian Elongin A and Its Role in Stress Response

The TFIIS and TFIIS-like genes from Medicago truncatula are involved in oxidative stress response

Genotoxic stress and DNA repair in plants: emerging functions and tools for improving crop productivity

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