The TFIIS and TFIIS-like genes from Medicago truncatula are involved in oxidative stress response

Macovei, Anca; Balestrazzi, Alma; Confalonieri, Massimo; Buttafava, A.; Carbonera, Daniela

doi:10.1016/j.gene.2010.09.004

Cited by 35 publications

(16 citation statements)

References 51 publications

(58 reference statements)

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“…No information is currently available concerning the possible involvement of the TFIIS gene in the antioxidant response in plants, except for the report from Macovei et al (2010b). In this case, the up-regulation of the MtTFIIS gene in both the aerial parts and roots of barrel medic plantlets challenged with copper and PEG6000, respectively, were clearly demonstrated.…”

Section: Discussionmentioning

confidence: 89%

“…In plants, the TFIIS gene was characterized only recently (Grasser et al, 2009;Macovei et al, 2010b). In A. thaliana, the lack of TFIIS gene expression significantly reduced the dormant stage of fully developed seeds before germination, suggesting that the TFIIS protein might be involved in the regulation of seed dormancy by modulating those genes which play a key role in this process.…”

Section: Genementioning

confidence: 99%

“…It is worth noting that plant cells contain a novel gene, isolated for the first time in barrel medic and named MtTFIIS-like, encoding a protein of unknown function that shares common features with TFIIS. Both the MtTFIIS and MtTFIIS-like genes are up-regulated in response to copper-and PEG-mediated stress and also during seed imbibition (Macovei et al, 2010b).…”

Section: Genementioning

confidence: 99%

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Seed imbibition in Medicago truncatula Gaertn.: Expression profiles of DNA repair genes in relation to PEG-mediated stress

Balestrazzi

Confalonieri

Macovei

et al. 2011

Journal of Plant Physiology

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Section: Discussionmentioning

confidence: 89%

Section: Genementioning

confidence: 99%

Section: Genementioning

confidence: 99%

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Seed imbibition in Medicago truncatula Gaertn.: Expression profiles of DNA repair genes in relation to PEG-mediated stress

Balestrazzi

Confalonieri

Macovei

et al. 2011

Journal of Plant Physiology

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“…AFK34552.1; Medtr1g048990) that has 95% identity to a Medicago sativa FeSOD (GenBank accession no. AAL32441.1), predicted to be localized to the chloroplast (Alscher et al, 2002;Rubio et al, 2004;Asensio et al, 2012), as well as a Cu/ ZnSOD (CytoCu/ZnSOD; Medtr7g114240) predicted to be cytoplasmic (Macovei et al, 2011) did not show a difference in gene expression between latd and wildtype roots (Supplemental Fig. S5).…”

Section: Decreasing Ros Levels Can Increase Cell Elongation In Latd Rmentioning

confidence: 98%

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

2014

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Abscisic acid (ABA) modulates root growth in plants grown under normal and stress conditions and can rescue the root growth defects of the Medicago truncatula lateral root-organ defective (latd) mutant. Here, we demonstrate that reactive oxygen species (ROS) function downstream of ABA in the regulation of root growth by controlling cell elongation. We also show that the MtLATD/NUMEROUS INFECTIONS AND POLYPHENOLICS (NIP) nitrate transporter is required for ROS homeostasis and cell elongation in roots and that this balance is perturbed in latd mutants, leading to an excess of superoxide and hydrogen peroxide and a corresponding decrease in cell elongation. We found that expression of the superoxide-generating NADPH oxidase genes, MtRbohA and MtRbohC (for respiratory burst oxidase homologs), is increased in latd roots and that inhibition of NADPH oxidase activity pharmacologically can both reduce latd root ROS levels and increase cell length, implicating NADPH oxidase function in latd root growth defects. Finally, we demonstrate that ABA treatment alleviates ectopic ROS accumulation in latd roots, restores MtRbohC expression to wild-type levels, and promotes an increase in cell length. Reducing the expression of MtRbohC using RNA interference leads to increased root elongation in both wild-type and latd roots. These results reveal a mechanism by which the MtLATD/NIP nitrate transporter and ABA modulate root elongation via superoxide generation by the MtRbohC NADPH oxidase.

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“…In addition, the activities of antioxidant enzymes and the metabolism of antioxidants such as glutathione and flavonoids significantly increase in plants exposed to heavy metal stress [4, 19, 20]. Genes involved in heavy metal responses have been widely studied in different plant species [15, 21–24]; however, the genome-wide network for gene expression and interactions in response to heavy metals are still relatively unknown. Recent studies using next-generation sequencing technology identified a number of microRNAs and their corresponding targets, which were genes whose expression is sensitive to heavy metal stress [20, 25–27].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and physiological analyses of Medicago sativa L. roots in response to lead stress

Wang

Zhang

et al. 2017

PLoS ONE

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Lead (Pb) is one of the nonessential and toxic metals that threaten the environment and human health. Medicago sativa L. is a legume with high salt tolerance and high biomass production. It is not only a globally important forage crop but is also an ideal plant for phytoremediation. However, the biological and molecular mechanisms that respond to heavy metals are still not well defined in M. sativa. In this study, de novo and strand-specific RNA-sequencing was performed to identify genes involved in the Pb stress response in M. sativa roots. A total of 415,350 unigenes were obtained from the assembled cDNA libraries, among which 5,416 were identified as significantly differentially expressed genes (DEGs) (false discovery rate < 0.005) between cDNA libraries from control and Pb-treated plants. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs showed they mainly clustered with terms associated with binding, transport, membranes, and the pathways related to signal and energy metabolism. Moreover, a number of candidate genes included antioxidant enzymes, metal transporters, and transcription factors involved in heavy metal response were upregulated under Pb stress. Quantitative real-time PCR(qRT-PCR) validation of the expression patterns of 10 randomly selected candidate DEGs were consistent with the transcriptome analysis results. Thus, this study offers new information towards the investigation of biological changes and molecular mechanisms related to Pb stress response in plants.

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The TFIIS and TFIIS-like genes from Medicago truncatula are involved in oxidative stress response

Cited by 35 publications

References 51 publications

Seed imbibition in Medicago truncatula Gaertn.: Expression profiles of DNA repair genes in relation to PEG-mediated stress

Seed imbibition in Medicago truncatula Gaertn.: Expression profiles of DNA repair genes in relation to PEG-mediated stress

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

Transcriptomic and physiological analyses of Medicago sativa L. roots in response to lead stress

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