Functional characterization and expression study of sugarcane MYB transcription factor gene PEaMYBAS1 promoter from Erianthus arundinaceus that confers abiotic stress tolerance in tobacco

Kharte, Sonali B.; Watharkar, Anuprita D.; Shingote, Prashant Raghunath; Chandrashekharan, Sharanya; Pagariya, Madhuri Chandrakant; Kawar, Prashant G.; Govindwar, Sanjay P.

doi:10.1039/c5ra21414a

Cited by 8 publications

(4 citation statements)

References 63 publications

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“…In higher plants, the MYB superfamily is a highly specialized and broad group of R2R3-MYBs containing various MYB repeats in their sequences unveiling DNA-binding activity (Baldoni et al 2015). The R2R3-MYB proteins have a fundamental role in regulating numerous plant functions, including growth, metabolism, and ABA-mediated reactivity to biotic or abiotic stimuli (Kharte et al 2016). The recent advancements in genetic and molecular approaches have promoted the large-scale characterization of functional MYB proteins, especially R2R3-type classes in crop plants including soybean, rice, and maize (Roy 2016).…”

Section: Myb Transcription Factorsmentioning

confidence: 99%

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

et al. 2019

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Key message We describe here the recent developments about the involvement of diverse stress-related proteins in sensing, signaling, and defending the cells in plants in response to drought or/and heat stress. Abstract In the current era of global climate drift, plant growth and productivity are often limited by various environmental stresses, especially drought and heat. Adaptation to abiotic stress is a multigenic process involving maintenance of homeostasis for proper survival under adverse environment. It has been widely observed that a series of proteins respond to heat and drought conditions at both transcriptional and translational levels. The proteins are involved in various signaling events, act as key transcriptional activators and saviors of plants under extreme environments. A detailed insight about the functional aspects of diverse stress-responsive proteins may assist in unraveling various stress resilience mechanisms in plants. Furthermore, by identifying the metabolic proteins associated with drought and heat tolerance, tolerant varieties can be produced through transgenic/recombinant technologies. A large number of regulatory and functional stress-associated proteins are reported to participate in response to heat and drought stresses, such as protein kinases, phosphatases, transcription factors, and late embryogenesis abundant proteins, dehydrins, osmotins, and heat shock proteins, which may be similar or unique to stress treatments. Few studies have revealed that cellular response to combined drought and heat stresses is distinctive, compared to their individual treatments. In this review, we would mainly focus on the new developments about various stress sensors and receptors, transcription factors, chaperones, and stress-associated proteins involved in drought or/ and heat stresses, and their possible role in augmenting stress tolerance in crops.

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Section: Myb Transcription Factorsmentioning

confidence: 99%

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

et al. 2019

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“…Some genes related to drought resistance have been cloned from sweetcane (Kharte et al, 2016;Liu, Hu, Yao, Xu, & Xing, 2016;Zhang et al, 2007). The gene HSP70 from sweetcane had been shown to play an important role in Sugarcane (Saccharum spp.…”

Section: Droughtmentioning

confidence: 99%

Sweetcane (Erianthus arundinaceus) as a native bioenergy crop with environmental remediation potential in southern China: A review

Wang

et al. 2019

GCB Bioenergy

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Sweetcane (Erianthus arundinaceus [Retzius] Jeswiet) is an ecologically dominant warm‐season perennial grass native to southern China. It traditionally plays an important role in sugarcane breeding due to its excellent biological traits and genetic relatedness to sugarcane. Recent studies have shown that sweetcane has a great potential in bioenergy and environmental remediation. The objective of this paper is to review the current research on sweetcane biology, phenology, biogeography, agronomy, and conversion technology, in order to explore its development as a bioenergy crop with environmental remediation potential. Sweetcane is resistant to a variety of stressors and can adapt to different growth environments. It can be used for ecological restoration, soil and water conservation, contaminated land repairing, nonpoint source pollutants barriers in buffer strips along surface waters, and as an ornamental and remediation plant on roadsides and in wetlands. Sweetcane exhibits higher biomass yield, calorific value and cellulose content than other bioenergy crops under the same growth conditions, thereby indicating its superior potential in second‐generation biofuel production. However, research on sweetcane as a bioenergy plant is still in its infancy. More works need be conducted on breeding, cultivation, genetic transformation, and energy conversion technologies.

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“…Genetic engineering offers some advantages over traditional methods of breeding, and Agrobacterium -meditated transformation of genes has become a gold standard (Shingote et al 2015 ; Kharte et al 2016 ), a method that integrates fewer copies of trans genes into plants compared to the biolistic method and is, therefore, the preferred method for obtaining stable expression of trans genes and avoiding trans gene silencing (Joyce et al 2010 ; Ramesh et al 2004 ). In this process, only the T-DNA region of the vector is transferred, a region engineered to encompass a selectable marker, a reporter gene, and the genes of interest, which are then transferred from the bacterium to the host plant’s nuclear genome.…”

Section: Introductionmentioning

confidence: 99%

Parameters influencing Agrobacterium-mediated transformation system in safflower genotypes AKS-207 and PKV Pink

et al. 2016

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Shoot regeneration in safflower (Carthamus tinctorius ‘AKS 207’ and ‘PKV Pink’) genetically transformed using Agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. Transformation frequency was promising with 8–10-day-old cotyledonary leaf explants. Therefore, explants of that age cultured on Agrobacterium minimal medium (AB) containing 100 µM acetosyringone were infected with Agrobacterium (cell titre 0.5 OD600nm) for 15 min followed by 48 h of co-cultivation on kanamycin-enriched medium (50 mg/L). Transformation of the shoots was confirmed using β-glucuronidase (GUS) histochemical assay and polymerase chain reaction (PCR). With the transformation protocol thus optimized, the transformation frequency as determined using GUS assays was 54.0 % for AKS 207 and 47.6 % for PKV Pink. The corresponding figures using PCR were 27.0 and 33.3 %. The transformed shoots required 10–14 weeks of culture initiation but produced very few roots.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0497-4) contains supplementary material, which is available to authorized users.

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Functional characterization and expression study of sugarcane MYB transcription factor gene PEaMYBAS1 promoter from Erianthus arundinaceus that confers abiotic stress tolerance in tobacco

Abstract: This work provides a thorough understanding about the function ofcis-acting elements regarding drought, salt, cold and wounding stress.

Cited by 8 publications

References 63 publications

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

Sweetcane (Erianthus arundinaceus) as a native bioenergy crop with environmental remediation potential in southern China: A review

Parameters influencing Agrobacterium-mediated transformation system in safflower genotypes AKS-207 and PKV Pink

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