Functional characteristics of apheresis‐derived platelets treated with ultraviolet light combined with either amotosalen‐HCl (S‐59) or riboflavin (vitamin B<sub>2</sub>) for pathogen‐reduction

Picker, Susanne M.; Oustianskaia, Larissa; Schneider, Volker; Gathof, Birgit

doi:10.1111/j.1423-0410.2009.01176.x

Cited by 74 publications

(108 citation statements)

References 35 publications

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“…TRAP-6 stimulation induced an increase in P-selectin and PS on platelets adhering to fibrinogen until day 7 but did not have any additional effect on the expression of P-selectin or PS on platelets adhering to collagen beads. The reduced response to TRAP-6 at the end of storage (lower fibrinogen binding and lower expression of P-selectin on adherent platelets) is in accordance to other studies using aggregometry to assess platelet function [25][26][27].…”

Section: Discussionsupporting

confidence: 73%

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by apheresis technique (N = 7).Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage.Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analysed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p < 0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p < 0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p < 0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation.We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.3

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Section: Discussionsupporting

confidence: 73%

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

et al. 2014

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“…The most likely explanation for the discrepancies between our results and the study by Stivala et al 1 and others [4][5][6] is the fact that we isolated platelets from the storage milieu in order to explore their intrinsic functional properties, independently of the storage milieu which may have an inhibitory-yet-reversible effect on platelet responsiveness. We also looked at the reactivity of platelets in their storage milieu and, indeed, found there was already inhibition of platelet aggregation in response to ADP on Day 1.5, and to collagen on Day 6.5, which was, however, similar between the untreated and the IBS-PCs 2 (see Online Supplementary Figure S2B).…”

contrasting

confidence: 55%

“…5,6 In addition, in our study, the pH values of the storage milieu remained similar in both untreated and IBS-PCs, while Stavila et al reported a significant decrease in pH in IBS-treated PCs compared to untreated units by Day 1.5. Thus, independently of the washing procedure, differences in the overall initial collection procedure and/or storage conditions may play a role and could explain such differences between centers.…”

mentioning

confidence: 58%

Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance

2017

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Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearanceWith interest we read the paper by Stivala et al., 1 recently published in Haematologica, evaluating the structural and functional consequences induced by Amotosalen/UVA treatment using the Intercept Blood System (IBS) on platelets from apheresis platelet concentrates (PCs) during storage. This study provides results of early diminished platelet function in IBS-treated PCs as compared to conventional PCs, i.e., reduced aggregation response to collagen or thrombin and adhesion to collagen or vWF under flow, increased platelet apoptosis, MAPK p38 activation, and glycoprotein Ibα (GPIbα) shedding and enhanced clearance from the circulation of mice.These results strikingly contrast with our previous published study 2 which evaluated the impact of IBS on platelets isolated from buffy coat PCs, washed and suspended in Tyrode's buffer. Surprisingly, Stivala et al.

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“…Further, there is an unbalance between APCs gamma-irradiated at the day of transfusion and those irradiated in advance in favor of the former. It would be of interest to evaluate in more detail both the association between transfusion efficacy and the time frame between gamma irradiation of APCs and transfusion as well as the effect of treatment by riboflavin or amotosalen-HCI plus ultraviolet light also including the time frame between irradiation and transfusion, which in the later procedure could be up to 7 days [26]. …”

Section: Discussionmentioning

confidence: 99%

Transfusion Efficacy of Apheresis Platelet Concentrates Irradiated at the Day of Transfusion Is Significantly Superior Compared to Platelets Irradiated in Advance

Julmy

Ammann

Fontana

et al. 2014

Transfus Med Hemother

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Background: Gamma irradiation is currently the standard care to avoid transfusion-associated graft-versus-host disease. Guidelines on gamma irradiation of blood components state that platelets (PLTs) can be irradiated at any stage in their 5-day storage and can thereafter be stored up to their normal shelf life of 5 days after collection. In this study, we explored whether the timing of irradiation has an effect on transfusion efficacy of apheresis PLT concentrates (APCs). Methods: Based on the 1-hour percent PLT recovery (PPR_1h), transfusion efficacy of 1,000 eligible APCs transfused to 144 children were evaluated retrospectively. PPR_1h was compared in transfused APCs irradiated at the day of transfusion and APCs irradiated in advance. Results: In univariate analysis, transfusion efficacy of APCs irradiated in advance was significantly lower than that of APCs irradiated at the day of transfusion (mean PPR_1h 27.7 vs. 35.0%; p = 0.007). This was confirmed in multivariate analysis (p = 0.030). Compared to non-irradiated APCs, transfusion efficacy of APCs irradiated at the day of transfusion was not significantly inferior (mean difference -2.8%; 95% CI -6.1 to 0.5%; p = 0.092), but APCs irradiated in advance were clearly less efficient (mean difference -8.1%; 95% CI -12.2 to -4.0%; p < 0.001). Conclusion: Our data strongly support that APCs should not be irradiated in advance, i.e., ≥24 h before transfusion.

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Functional characteristics of apheresis‐derived platelets treated with ultraviolet light combined with either amotosalen‐HCl (S‐59) or riboflavin (vitamin B₂) for pathogen‐reduction

Cited by 74 publications

References 35 publications

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance

Transfusion Efficacy of Apheresis Platelet Concentrates Irradiated at the Day of Transfusion Is Significantly Superior Compared to Platelets Irradiated in Advance

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Functional characteristics of apheresis‐derived platelets treated with ultraviolet light combined with either amotosalen‐HCl (S‐59) or riboflavin (vitamin B2) for pathogen‐reduction

Cited by 74 publications

References 35 publications

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads

Amotosalen/UVA pathogen inactivation technology reduces platelet activability, induces apoptosis and accelerates clearance

Transfusion Efficacy of Apheresis Platelet Concentrates Irradiated at the Day of Transfusion Is Significantly Superior Compared to Platelets Irradiated in Advance

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Functional characteristics of apheresis‐derived platelets treated with ultraviolet light combined with either amotosalen‐HCl (S‐59) or riboflavin (vitamin B₂) for pathogen‐reduction