Functional annotation of the genome unravels probiotic potential of Bacillus coagulans HS243

Abstract

Spore forming Bacillus species are widely used as probiotics for human dietary supplements and in animal feeds. However, information on genetic basis of their probiotic action is obscure. Therefore, the present investigation was undertaken to elucidate probiotic traits of B. coagulans HS243 through its genome analysis. Genome mining revealed the presence of an arsenal of marker genes attributed to genuine probiotic traits. In silico analysis of HS243 genome revealed the presence of multi subunit ATPases, ADI pathway genes, chologlycine hydrolase, adhesion proteins for surviving and colonizing harsh gastric transit. HS243 genome harbored vitamin and essential amino acid biosynthetic genes, suggesting the use of HS243 as a nutrient supplement. Bacteriocin producing genes highlighted the disease preventing potential of HS243. Thus, this work established that HS243 possessed the genetic repertoire required for surviving harsh gastric transit and conferring health benefits to the host which were further validated by wet lab evidences.

Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms

“…are currently used as probiotic dietary supplements [15]. One of the reasons is that spore-forming probiotics are highly resistant to extreme environmental conditions [16]. Bacillus spp.…”

Integrated genome-based probiotic relevance and safety evaluation of Lactobacillus reuteri PNW1

“…A total of 12 coding sequences were found in the genome of L. reuteri PNW1, predictably encoding for both D-lactate dehydrogenase (EC 1.1.1.28) and L-lactate dehydrogenase (EC 1.1.1.27) which are responsible for lactic acids production. In addition, a promising probiotic candidate with inherent ability for producing bioactive peptides and hydrogen peroxide would be an added advantage in antagonising neighbouring pathogens within the gut ecosystem [39]. The in silico analysis of the entire genome for putative secondary metabolites, coupled with the functional annotation of the coding sequences within the L. reuteri PNW1 genome assembly, revealed the occurrence of CDS for bacteriocin helveticin J and S-ribosylhomocysteinelyase (EC 4.4.1.21) @Autoinducer-2 production protein LuxS, a protein involved in the secretion of bioactive peptides.…”

“…reuteri PNW1, predictably encoding for both D-lactate dehydrogenase (EC 1.1.1.28) and L-lactate dehydrogenase (EC 1.1.1.27) which are responsible for lactic acids production. In addition, a promising probiotic candidate with inherent ability for producing bioactive peptides and hydrogen peroxide would be an added advantage in antagonising neighbouring pathogens within the gut ecosystem [ 39 ]. The in silico analysis of the entire genome for putative secondary metabolites, coupled with the functional annotation of the coding sequences within the L .…”

Criterios y estrategias tecnológicas para la incorporación y supervivencia de probióticos en frutas, cereales y sus derivados

“…Otra especie que destaca actualmente por su resistencia a ambientes adversos, es el Bacillus coagulans, conocido anteriormente como Lactobacillus sporogenes. Este microorganismo comparte características, tanto de la familia Lactobacillaceae como de la Bacillaceae (Kapse, Engineer, Gowdaman, Wagh & Dhakephalkar, 2018). B. coagulans produce la bacteriocina es necesario realizar una identificación fenotípica y genotípica precisa del microorganismo (género, especie y cepa).…”

“…Es importante recordar que el efecto probiótico de las bacterias es cepa específico (Bagon et al, 2018). Por tanto el genoma de diferentes cepas de B. coagulans, y sus propiedades probióticas, están siendo actualmente investigadas con la finalidad de determinar si son aptas para su uso en alimentos (Yao, Gao & Zhang, 2016;Kapse, Engineer, Gowdaman, Wagh & Dhakephalkar, 2018).…”