Functional and Ultrastructural Changes in Cryopreserved Corneas

Horn, Diane L. Van; Edelhauser, Henry F.; DeBruin, Janet

doi:10.1001/archopht.1973.01000050314016

Cited by 15 publications

(4 citation statements)

References 3 publications

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“…9 Other studies have suggested that hypothermia slows down tissue metabolism rate and enzymatic reactions. 16 This statement is consistent with a study by Wan et al, in which corneal endothelial cells in the 4 o C group had a higher endothelial cell density than the 24 o C room temperature group on day 1 postoperative. However, there was no significant difference in endothelial morphology between the two groups on the 7 th postoperative day.…”

Section: Discussionsupporting

confidence: 92%

Expression of Na+/K+ATPase and changes of corneal endothelium cell density after phacoemulsification with hypothermic perfusion

et al. 2022

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Background: Intraocular hypothermic perfusion can be used as an alternative to reduce corneal endothelial cell damage in phacoemulsification procedures. This study aimed to analyze the effect of hypothermic intraocular perfusion on cell density and expression of the Na+/K+-ATPase on corneal endothelial cells after exposure to ultrasonic phacoemulsification energy. Methods: Sixteen New Zealand white rabbits (n=16 eyes) were randomly divided into two groups and exposed to phacoemulsification ultrasonic energy. The control group received room-temperature Intraocular Balanced Salt Solution (BSS) hyperthermic perfusion (24oC) and the treatment group received low-temperature intraocular BSS hypothermic perfusion (4oC). Corneal endothelial cell density (ECD) was measured before and 1 day after surgery using a specular microscope. On the first postoperative day, the expression of Na+/K+ATPase was examined using immunohistochemical antibody staining. Data were analyzed using SPSS version 26 for Windows. Results: There was no statistically significant difference in the expression of corneal endothelial Na+/K+-ATPase between the control and treatment groups (p=0.053). There was no significant difference in corneal endothelial cell density changes between the control and treatment groups (p=0.115). There was no correlation between changes in cell density and corneal endothelial Na+/K+ATPase expression in the control and treatment groups (p=0.216). Conclusion: The effect of intraocular hypothermic perfusion on phacoemulsification procedures remains controversial. Endothelial cell density decreased in hypothermic perfusion after phacoemulsification and Na+/K+ATPase expression was not significantly affected by hypothermic perfusion.

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Section: Discussionsupporting

confidence: 92%

Expression of Na+/K+ATPase and changes of corneal endothelium cell density after phacoemulsification with hypothermic perfusion

et al. 2022

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“…In a study conducted on rabbit eyes, it was observed that cooled irrigating solutions lowered fibrin production and postoperative inflammation as compared with solutions at room temperature. 21 It has been hypothesized by others 9 that the hypothermic effect may be due to a generalized slowing down in the tissue metabolism and enzymatic reactions as well as decreased uveal blood flow. Our observations contradict these findings.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6][7][8] Hypothermic conditions lower metabolic rates, thereby decreasing the demand for oxygen. 9 Recent work has extended this interest to the effects of intraoperative hypothermic perfusion on the retinovascular tissue. 10 The functional and ultrastructural changes that occur in the cornea due to controlled freezing have been studied extensively.…”

Section: Introductionmentioning

confidence: 99%

Effect of room temperature and cooled intraocular irrigating solution on the cornea and anterior segment inflammation after phacoemulsification: a randomized clinical trial

Praveen

Vasavada

Shah

et al. 2008

Eye

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Purpose To compare the effects of cooled and room temperature BSS Plus solution on the central corneal thickness (CCT), anterior segment inflammation, and corneal endothelium in patients undergoing phacoemulsification. Methods In this prospective, randomized study, 70 consecutive patients undergoing phacoemulsification were assigned to one of two groups: Group 1 (controls) (n ¼ 35) received BSS Plus at a room temperature of 231C and Group 2 (cases; n ¼ 35) received BSS Plus cooled at 101C. In both the groups, the percentage of difference for CCT, absolute change in endothelial cell density, and coefficient of variation (CV) from preoperative to postoperative periods was analysed. The presence of anterior chamber flare and cells were compared postoperatively between the two groups. Results There was no significant difference in percentage change for CCT from preoperative to postoperative days 1, 7, and 30 between the two groups (P ¼ 0.30, P ¼ 0.56, and P ¼ 0.52). There was no statistically significant difference in the ECD loss (P ¼ 0.19) and CV (P ¼ 0.88) at 1 month between the two groups. The influence of the solutions on anterior chamber flare and cells was not statistically significant between the two groups on the first postoperative day (P ¼ 0.17, P ¼ 0.11). Conclusions The temperature of the BSS Plus solution did not affect any parameter of the study. The use of moderately cooled BSS had no detectable effect and benefit on the outcome of the surgery.

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“…Ό μεταβολισμός των ενδοθηλιακών κυττάρων βλάπτεται ή δέ πρόσληψις οξυγόνου βρέθηκε νά είναι κατά 50% ελαττωμένη σέ ιστούς πού συντηρήθηκαν μέ τήν μέθοδο K-C σέ σχέσι μέ τους φρέσκους κερατοειδείς (105).…”

Section: κρυοσυντηρηση βιωσιμου ιστου για διαμπερεις μεταμοσχευσειςunclassified

Ιστομορφολογικες Αλλοιωσεις Των Μοσχευματων Του Κερατοειδους Μετα Απο Κρυοσυντηρηση

Συρμποπουλοσ¹

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ΑΠΟΤΕΛΕΣΜΑΤΑ 1. Τοξικότης σέ 35°C 2. Τοξικότης σέ 0°C 3. Μορφολογία καί Λειτουργικότητα σέ ψύξη στους -15°C, -20°C, -30°C 77 4. Κρυοπροοτατευτικά διαλύματα-Ποσοοτιαϊες αναλογίες 5. Ρυθμοί ψύξης 6. ' Απόψυξη 85 7."Απομάκρυνση κρυοπροστατευτικών παραγόντων μετά τήν απόψυξη 8. Ψύξη «έν στεγνώ» 9. Ψύξη χωρίς κρυοπροοτατευτικά 10. Μορφολογικές-ίοτολογικές παρατηρήσεις 89 α. Χορήγηση κρυοπροστατευτικών σέ υψηλές θερμοκρασίες β. » » » 0°C 89 γ. Ψύξη στους -15° μέ τελική πυκνότητα 12,5% D.M.S.O. καί 12,5% P.V. (έν ύγρώ) 90 δ. Ψύξη στους -30°C 90 ε. Ψύξη στους -30°C μέ κανονική διαπέραση άπό όλα τά κρυοπροοτατευτικά διάλυμα «έν στεγνώ» καί μέ «πλατώ» ψύξη 93 ζ. Ψύξη μέ υπερβολικά ταχύ ρυθμό, χωρίς «πλατώ» καί μέ υπερβολικά «βραδύ» ρυθμό 95 η. Ψύξη «έν στεγνώ» μέ τίς προτεινόμενες αναλογίες προστα τευτικών καί μέ τόν προτεινόμενο ρυθμό ατούς -196°C 97 θ. Βλάβες τοΰ επιθηλίου καί του στρώματος 1Q . ΣΥΖΗΤΗΣΗ 105 ΣΥΜΠΕΡΑΣΜΑΤΑ-ΠΕΡΙΛΗΨΗ 113 SUMMARY 115 ΒΙΒΛΙΟΓΡΑΦΙΑ 117 Τό 1982 πήρα υποτροφία τών Special Trustees τής Avon Health District.

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Functional and Ultrastructural Changes in Cryopreserved Corneas

Cited by 15 publications

References 3 publications

Expression of Na+/K+ATPase and changes of corneal endothelium cell density after phacoemulsification with hypothermic perfusion

Expression of Na+/K+ATPase and changes of corneal endothelium cell density after phacoemulsification with hypothermic perfusion

Effect of room temperature and cooled intraocular irrigating solution on the cornea and anterior segment inflammation after phacoemulsification: a randomized clinical trial

Ιστομορφολογικες Αλλοιωσεις Των Μοσχευματων Του Κερατοειδους Μετα Απο Κρυοσυντηρηση

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