Functional and ultrastructural alterations of autologous platelets labeled with 111In-oxine

Bernard, P; M, Bazan; C, Foa; Mountaz, Kébir; Juhan-Vague, I.

doi:10.1007/bf00252890

Cited by 12 publications

(3 citation statements)

References 15 publications

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“…The radioisotopic method used has been evaluated by several investigators. It has been shown that In-1 11 oxine is firmly bound to platelets and that dissociation of In-111 oxine from the platelets does not occur in viuo (29,30). I n addition, a previous study on platelet sequestration in the lungs and liver in sheep showed that gamma camera images correlated well with direct tissue activity measurements (25).…”

Section: Discussionmentioning

confidence: 89%

Effects of pentoxifylline on hemodynamics, gas exchange and multiple organ platelet sequestration in experimental endotoxic shock

Sigurdsson

Youssef

1993

Acta Anaesthesiol Scand

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In an intensive care setting we studied the effects of pentoxifylline on hemodynamics, gas-exchange and platelet sequestration in multiple organs in three groups of sheep exposed to endotoxin shock (n = 7 in each). Group P-E was given pentoxifylline before and group E-P after E. coli endotoxin infusion, while group E received normal saline (controls). The endotoxin infusion caused a three-fold increase in pulmonary artery pressure (PAP) and a significant decrease in mean arterial pressure (25-30%; MAP), respiratory compliance (CT; 60%) and arterial oxygen tension (65-70%; Pao2) in all groups after 30 min. After 4 h MAP had improved significantly in the pretreated animals (group P-E) and arterial pH, Pao2 and CT improved in both pentoxifylline-treated groups compared with the controls. On the other hand, the effects of endotoxin on PAP and cardiac index were not significantly influenced by pentoxifylline treatment. In addition, there was a pronounced platelet sequestration in the lungs and in the liver in groups E and E-P during the 4 h study, but in the pretreated group (group P-E) the changes were significantly less marked (P < 0.01). The wet-to-dry weight ratios of the lungs were significantly lower in both pentoxifylline-treated groups compared with the controls (P < 0.01). It was concluded that pentoxifylline modified the effects of endotoxin on hemodynamics, gas exchange and platelet sequestration in the lungs and liver in sheep when it was given prior to endotoxin. However, when it was given after hemodynamic and respiratory signs of shock had appeared, the effects were more moderate.

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Section: Discussionmentioning

confidence: 89%

Effects of pentoxifylline on hemodynamics, gas exchange and multiple organ platelet sequestration in experimental endotoxic shock

Sigurdsson

Youssef

1993

Acta Anaesthesiol Scand

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“…HillZobel et al (;1987) used rabbit platelets labelled with indium-Ill oxine-sulphate, tropolone or MPO and re-ported on marked decreases in platelet survival and initial recovery in platelets labelled without plasma. With 7.4 MBq per 109 platelets labelled in saline, Bernard et al (1983) found only slight morphological alterations on scanning electron microscopy and also described about a 15% decrease in aggregability compared with unlabelled platelets. Other studies indicated unimpaired platelet survival with platelets labelled outside of plasma (Heaton et al 1979).…”

Section: Discussionmentioning

confidence: 93%

Functional alterations of human platelets following indium-111 labelling using different incubation media and labelling agents

Isaka

Kimura²,

Matsumoto

et al. 1991

Eur J Nucl Med

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Human platelets were labelled in the absence or presence of plasma using indium-111 labelled oxine sulphate, tropolone or 2-mercaptopyridine-N-oxide (MPO). Under in vitro and in vivo conditions, platelet functions were evaluated by measuring their aggregability, survival, recovery and early distribution. High labelling efficiency was achieved in saline labelling, whereas with plasma labelling, it was necessary to concentrate the platelet-rich plasma to 4.8 x 10(6) platelets/microliters. The aggregation of platelets labelled in plasma or saline was compared with that of controls; platelets labelled in saline showed lower aggregability in 2 microM ADP but not in 5 microM ADP nor with collagen. No significant differences in platelet survival and recovery were noted between platelets labelled in plasma and those labelled in saline. Our results indicate that partial loss of ADP aggregability in vitro does not influence the in vivo viability of platelets labelled in saline. Scintigraphic studies showed that platelets labelled in a saline medium were temporarily sequestrated in the liver but not in the spleen or heart. Thus, platelet labelling in saline does not affect platelet function adversely, but platelets labelled in plasma are more desirable for assessing the early distribution of platelets in the reticuloendothelial system.

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“…Earlier experiments have shown that Indium-Ill oxine is firmly bound to platelets and that dissociation of In-111 oxine from platelets does not occur in vivo [13][14][15]. Hence, the radioactivity measured in those organs (lung, liver, and kidney) reflects the number of platelets sequestered in those organs.…”

Section: Discussionmentioning

confidence: 97%

Early and late platelet sequestration in different organs during endotoxic shock

1988

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Using a sheep model, platelet sequestration in lungs, liver, kidney, spleen, and brain was studied after the injection of Indium-111-oxine-labeled platelets and E. coli lipopolysaccharide. Immediately following endotoxin-induced shock, platelet sequestration was observed in lungs, liver, and kidney with corresponding decrease in measured Indium-111-oxine platelets in spleen and circulating blood. There was also a decrease in platelet counts as measured in a phase contrast microscope. The early platelet sequestration in lung, liver, and kidney was followed by platelet disaggregation and another phase of platelet sequestration which continued until the death of the animal. No platelet sequestration was observed in the brain.

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Functional and ultrastructural alterations of autologous platelets labeled with 111In-oxine

Cited by 12 publications

References 15 publications

Effects of pentoxifylline on hemodynamics, gas exchange and multiple organ platelet sequestration in experimental endotoxic shock

Effects of pentoxifylline on hemodynamics, gas exchange and multiple organ platelet sequestration in experimental endotoxic shock

Functional alterations of human platelets following indium-111 labelling using different incubation media and labelling agents

Early and late platelet sequestration in different organs during endotoxic shock

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