Functional and taxonomic dynamics of an electricity-consuming methane-producing microbial community

Bretschger, Orianna; Carpenter, Kayla; Phan, Tony; Suzuki, Shino; Ishii, Shun’ichi; Grossi-Soyster, Elysse N.; Flynn, Michael; Hogan, John A.

doi:10.1016/j.biortech.2015.06.129

Cited by 37 publications

(38 citation statements)

References 34 publications

(40 reference statements)

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“…18 The reactors had a carbon based working electrode (WE) composed of three carbon cloth pieces stacked together (Zoltek, PW06, St. Louis, MO) with an apparent surface area of 28.5 cm 2 (total surface area of 85.5 cm 2 ). The counter electrode (CE) was constructed out of titanium mesh (Cleveland Wire cloth, 40×40 mesh, Cleveland, OH) and had an apparent surface area of 66 cm 2 .…”

Section: Methodsmentioning

confidence: 99%

“…18 The original BES enrichment started from 5 g of rice paddy soil (Koda Farms, South Dos Palos, CA) mixed with the modified 120 media under anoxic conditions, degassed with N 2 :CO 2 and operated potentiostatically at −700 mV vs Ag/AgCl. 18 The single graphite felt electrode from this BES was divided in half and each half was placed into two new sterile BES reactors of the type shown in Bretschger et al 2015. 18 Each BES subpassage was conducted for 7 to 14 days under batch operation.…”

Section: Methodsmentioning

confidence: 99%

“…18 The single graphite felt electrode from this BES was divided in half and each half was placed into two new sterile BES reactors of the type shown in Bretschger et al 2015. 18 Each BES subpassage was conducted for 7 to 14 days under batch operation. The time line of the experiment along with the points for the subpassages are shown in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

“…At the end of each batch cycle, an electrode from the electrode stack was subpassaged into a new sterile reactor to accelerate the enrichment of electron-consuming methane-producing communities. A schematic describing the subpassage process is shown in Bretschger et al 2015. 18 Briefly, medium was withdrawn from the reactors and a single electrode from the three-electrode stack was removed and sandwiched between two sterile electrodes in the new reactor.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, investigations into optimal microbial communities along with interspecies interactions have also gained attention in recent years. [16][17][18][19][20][21][22][23][24][25] The practical application of microbial electrosynthesis still requires a deeper understanding of how reactor design may fundamentally impact performance and overall microbial composition. In most cases, a microbial electrosynthesis reactor is a dual-chambered system composed of an anode electrode, a cathode electrode and a separator.…”

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confidence: 99%

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The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

Babanova

Carpenter

Phadke

et al. 2016

J. Electrochem. Soc.

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This study evaluated the influence of the membrane type on the performance of bioelectromethanogenesis reactors. The functional activities and taxonomic composition of bioelectrochemical systems (BES) with Nafion 117 or Ultrex CMI-7000 membranes were assessed. Functional activity was measured as methane production and current consumption rates throughout operation. Microbial biomass and phylogenetic diversity were characterized at strategic intervals related to the membrane type used. The Nafion-BES reactor showed the best performance in terms of current consumption and methane production in the early operational period and a strong selection for fermentative bacteria. However, the Nafion-BES was not able to sustain this activity over the course of 7 subpassages since methanogenic species were ultimately selected against and did not appear in the community composition for the last two subpassages. In contrast, the Ultrex-BES had a lower pH concentration gradient and lower overall current consumption activity; however, the methane production activity from the Ultrex-BES was equivalent or better than the Nafion-BES reactor and was sustained throughout the seven subpassages. The membrane type appeared to be responsible not only for differences in the electrochemical operation of the BESs but it also influenced microbial taxonomic composition and dynamics. Microbial electrosynthesis has been reported as a promising new technology for the synthesis of value-added products from CO 2 or other organic feedstocks.1 Microbial electrosynthesis relies on microbial population, applied potential, system design and the specific environmental conditions to define the final products and overall efficiencies of these systems.2-7 As a new technology, most of the studies in the area of microbial electrosynthesis address: i) proving the concept of using bacteria as electrocatalysts for targeted synthesis of a given product 8,9 and ii) understanding the mechanisms of "communication" between microbial species and electrode surfaces used as electron donors.10-13 Pure microbial cultures have primarily been used as they can provide selectivity of the synthesis process.2,12-15 However, from a practical standpoint, natural mixed microbial communities may provide a better strategy for bioelectrochemical systems exploring microbial electrosynthesis because they are more robust relative to operational changes, have greater metabolic capacity for converting/synthesizing complex substrates and are less susceptible to contamination during long-term operation. Therefore, investigations into optimal microbial communities along with interspecies interactions have also gained attention in recent years. [16][17][18][19][20][21][22][23][24][25] The practical application of microbial electrosynthesis still requires a deeper understanding of how reactor design may fundamentally impact performance and overall microbial composition. In most cases, a microbial electrosynthesis reactor is a dual-chambered system composed of an anode electrode, a cathode ele...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

Babanova

Carpenter

Phadke

et al. 2016

J. Electrochem. Soc.

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A Promising Strategy for Renewable Energy Recovery

Zhao

Zhang

2020

Bioelectrosynthesis

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Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium

et al. 2017

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Summary We report the first single‐chamber microbial electrochemical cell for conversion of CO2 to CH4, with an average CH4 production rate of 0.47 ± 0.05 mL day−1 cm−2 at an applied potential of 600 mV, utilizing a methanogenic microbial community collected from the formation water in the San Juan coal basin (Colorado, USA). CH4 production was only observed at the graphite rod cathode after an electrochemical pre‐treatment that facilitates biofilm formation. The carbon contained within the CH4 arose predominantly from the CO2 source, as verified by experiments during which the CO2 source was repeatedly turned off and on. Modest quantities of acetic acid and ethanol were also produced. DNA extraction and sequencing from the microbial community showed that from the Archaea kingdom, only 2 species survived prolonged exposure to CO2 and CH4 production, methanobacterium sp. (81.4%), and methanoculleus sp. (18.6%), while in the bacterial kingdom, anaerobaculum thermoterrnum (67.1%) was the predominant surviving species.

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Functional and taxonomic dynamics of an electricity-consuming methane-producing microbial community

Cited by 37 publications

References 34 publications

The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

A Promising Strategy for Renewable Energy Recovery

Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium

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Functional and taxonomic dynamics of an electricity-consuming methane-producing microbial community

Cited by 37 publications

References 34 publications

The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

The Effect of Membrane Type on the Performance of Microbial Electrosynthesis Cells for Methane Production

A Promising Strategy for Renewable Energy Recovery

Single-chamber microbial electrochemical cell for CH4production from CO2utilizing a microbial consortium

Contact Info

Product

Resources

About

Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium