Functional analysis of the α-neurotoxin, BmαTX14, derived from the Chinese scorpion, Buthus martensii Karsch

Wang, Kun; Yin, Shen; Lü, Meng; Yi, Hong; Dai, Chuan‐Chao; Xu, Xiu-Jing; Cao, Zhijian; Wu, Yingliang; Li, Wenxin

doi:10.1007/s10529-006-9155-y

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…Recombinant BmaTX14 was expressed in P. pastoris and its production was 120 mg/l, ten times of that in E. coli (Wang et al 2006). Similar results were obtained in our experiment.…”

Section: Discussionsupporting

confidence: 87%

Expression, purification and characterization of an analgesic peptide from Buthus martensii Karsch in Pichia pastoris

Yang

Zhu

Cheng

et al. 2009

World J Microbiol Biotechnol

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“…Recombinant BmaTX14 was expressed in P. pastoris and its production was 120 mg/l, ten times of that in E. coli (Wang et al 2006). Similar results were obtained in our experiment.…”

Section: Discussionsupporting

confidence: 87%

Expression, purification and characterization of an analgesic peptide from Buthus martensii Karsch in Pichia pastoris

Yang

Zhu

Cheng

et al. 2009

World J Microbiol Biotechnol

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“…The recombinant BmαTX14 expressed in Pichia pastoris could block sodium channel currents without affecting the gating kinetics in mouse trigeminal root ganglion neurons [35]. However, the recombinant BmαTX14 produced by the expression vector pET-28a could inhibit the fast inactivation of mNa v 1.4 sodium channels with the EC 50 of 82.3 ± 15.7 Nm [20].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity

Tian

Hong-lian

et al. 2014

Toxins

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Long-chain scorpion toxins with four disulfide bridges exhibit various pharmacological features towards the different voltage-gated sodium channel subtypes. However, the toxin production still remains a huge challenge. Here, we reported the effects of different expression vectors on the pharmacological properties of a novel toxin BmαTX47 from the scorpion Buthus martensii Karsch. The recombinant BmαTX47 was obtained using the expression vector pET-14b and pET-28a, respectively. Pharmacological experiments showed that the recombinant BmαTX47 was a new α-scorpion toxin which could inhibit the fast inactivation of rNav1.2, mNav1.4 and hNav1.5 channels. Importantly, the different expression vectors were found to strongly affect BmαTX47 pharmacological activities while toxins were obtained by the same expression and purification procedures. When 10 µM recombinant BmαTX47 from the pET-28a vector was applied, the values of I5ms/Ipeak for rNav1.2, mNav1.4 and hNav1.5 channels were 44.12% ± 3.17%, 25.40% ± 4.89% and 65.34% ± 3.86%, respectively, which were better than those values of 11.33% ± 1.46%, 15.96% ± 1.87% and 5.24% ± 2.38% for rNav1.2, mNav1.4 and hNav1.5 channels delayed by 10 µM recombinant BmαTX47 from the pET-14b vector. The dose-response experiments further indicated the EC50 values of recombinant BmαTX47 from the pET-28a vector were 7262.9 ± 755.9 nM for rNav1.2 channel and 1005.8 ± 118.6 nM for hNav1.5 channel, respectively. Together, these findings highlighted the important role of expression vectors in scorpion toxin pharmacological properties, which would accelerate the understanding of the structure-function relationships of scorpion toxins and promote the potential application of toxins in the near future.

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“…From the M . martensii library, a large number of putative toxins have been described, including NaTx (toxins specific for the sodium channels) [51,52,53,54,55,56], KTx (toxins specific for the potassium channels) [57,58,59,60,61,62,63], ClTx (toxins specific for chloride channels) [64], CaTx (toxins for ryanodine receptors) [65], Bpp-like (bradykinin-potentiating peptide-like) peptide [66], cytolytic peptide [67,68], and other functional peptides [69,70,71]. In 2009, Ma et al .…”

Section: Toxins From Chinese Scorpion Speciesmentioning

confidence: 99%

Overview of Scorpion Species from China and Their Toxins

Cao

et al. 2014

Toxins

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Scorpions are one of the most ancient groups of terrestrial animals. They have maintained a steady morphology over more than 400 million years of evolution. Their venom arsenals for capturing prey and defending against predators may play a critical role in their ancient and conservative appearance. In the current review, we present the scorpion fauna of China: 53 species covering five families and 12 genera. We also systematically list toxins or genes from Chinese scorpion species, involving eight species covering four families. Furthermore, we review the diverse functions of typical toxins from Chinese scorpion species, involving Na+ channel modulators, K+ channel blockers, antimicrobial peptides and protease inhibitors. Using scorpion species and their toxins from China as an example, we build the bridge between scorpion species and their toxins, which helps us to understand the molecular and functional diversity of scorpion venom arsenal, the dynamic and functional evolution of scorpion toxins, and the potential relationships of scorpion species and their toxins.

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Functional analysis of the α-neurotoxin, BmαTX14, derived from the Chinese scorpion, Buthus martensii Karsch

Cited by 11 publications

References 16 publications

Expression, purification and characterization of an analgesic peptide from Buthus martensii Karsch in Pichia pastoris

Expression, purification and characterization of an analgesic peptide from Buthus martensii Karsch in Pichia pastoris

Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity

Overview of Scorpion Species from China and Their Toxins

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