Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize

Yang, Fangfang; Ding, Xinhua; Chen, Jing; Shen, Yanting; Kong, Lingguang; Li, Ning; Zhang, Chu

doi:10.1186/s12870-017-1181-5

Cited by 11 publications

(12 citation statements)

References 49 publications

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“…The tissue-specific expression of transgenes plays a critical role in biotechnology crops because it avoids fitness costs caused by constitutive expression of target genes [ 9 ]. However, the information about the core cis-acting elements controlling tissue-specific expression is limited.…”

Section: Discussionmentioning

confidence: 99%

“…However, inducible promoters greatly increase the transcription level of genes under specific physical or chemical signals [ 5 ], this is different from constitutive promoters. At present, a great number of inducible promoters have been isolated, including light-inducible, heat-inducible and trauma-inducible [ 6 , 7 , 8 , 9 , 10 ]. Abiotic stress is a major obstacle for crop production, and the promoters of many genes related to abiotic stress have been cloned and applied in crop biotechnology.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Xue

Long

Zhao

et al. 2018

IJMS

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Promoters play a very important role in the initiation and regulation of gene transcription. Green-tissue promoter is of great significance to the development of genetically modified crops. Based on RNA-seq data and RT-PCR expression analysis, this study screened a gene, OrGSE (GREEN SPECIAL EXPRESS), which is expressed specifically in green tissues. The study also isolated the promoter of the OrGSE gene (OrGSEp), and predicted many cis-acting elements, such as the CAAT-Box and TATA-Box, and light-responding elements, including circadian, G-BOX and GT1 CONSENSUS. Histochemical analysis and quantification of GUS activity in transgenic Arabidopsis thaliana plants expressing GUS under the control of OrGSEp revealed that this promoter is not only green tissue-specific, but also light-inducible. The ability of a series of 5’-deletion fragments of OrGSEp to drive GUS expression in Arabidopsis was also evaluated. We found that the promoter region from −54 to −114 is critical for the promoter function, and the region from −374 to −114 may contain core cis-elements involved in light response. In transgenic rice expressing GUS under the control of OrGSEp, visualization and quantification of GUS activity showed that GUS was preferentially expressed in green tissues and not in endosperm. OrGSEp is a useful regulatory element for breeding pest-resistant crops.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Xue

Long

Zhao

et al. 2018

IJMS

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“…A promoter is a DNA sequence containing a variety of specific regulatory elements that regulates gene expression 20, 21 . To date, studies in plants have focused on constitutive promoters (with no spatiotemporal or tissue‐specific expression patterns), 22‐26 tissue‐specific promoters (with tissue‐specific expression patterns), 27‐30 and pathogen‐inducible promoters (with disease stress‐induced expression patterns) 31‐33 . Compared with the above promotor types, knowledge of insect‐inducible promoters lags far behind, and to date, only a very few insect‐inducible promoters have been reported 34‐37 …”

Section: Introductionmentioning

confidence: 99%

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

Wang

Han

et al. 2020

Pest Management Science

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BACKGROUNDPromoters play a key role in driving insect‐resistant genes during breeding of transgenic plants. In current transgenic procedures for breeding rice resistance to striped stem borer (Chilo suppressalis Walker, SSB), the constitutive promoter is used to drive the insect‐resistant gene. To reduce the burden of constitutive promoters on plant growth, isolation and identification of insect‐inducible promoters are particularly important. However, few promoters are induced specifically by insect feeding.RESULTSWe found rice hydroperoxide lyase gene (OsHPL2) (LOC_Os02g12680) was upregulated after feeding by SSB. We subsequently cloned the promoter of OsHPL2 and analysed its expression pattern using the β‐glucuronidase (GUS) reporter gene. Histochemical assays and quantitative analyses of GUS activity confirmed that P HPL2:GUS was activated by SSB, but did not respond to brown planthopper (Nilaparvata lugens Stål, BPH) infestation, mechanical wounding or phytohormone treatments. A series of 5′ truncated assays were conducted and three positive regulatory regions (−1452 to −1213, −903 to −624, and −376 to −176) induced by SSB infestation were identified. P2R123‐min 35S and P2TR2‐min 35S promoters linked with cry1C of transgenic plants showed the highest levels of Cry1C protein expression and SSB larval mortality.CONCLUSIONWe identified an SSB‐inducible promoter and three positive internal regions. Transgenic rice plants with the OsHPL2 promoter and its positive regions driving cry1C exhibited the expected larvicidal effect on SSB. Our study is the first report of an SSB‐inducible promoter that could be used as a potential resource for breeding insect‐resistant transgenic crops. © 2020 Society of Chemical Industry

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“…A previous study identified that the promoter of the R. solani-induced genes commonly harbors four types of cis-element sequences (GCTGA, TATAT, GTTGA, and TATTT) (33,34). To test whether the upregulated genes also carry the cis-element motifs, the 1.5 kb of promoter sequences from 31…”

Section: Identification Of Rhizoctonia Solani Responsive Cis-elementsmentioning

confidence: 99%

Transcriptome analysis of rice leaves in response to Rhizoctonia solani infection

Yuan¹,

Zhang²,

Wang³

et al. 2019

Preprint

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Background: Sheath blight disease (ShB) is one of the important diseases that severely affects rice production. However, the mechanism of defense against ShB remains unclear. To understand the molecular mechanism of rice defense to ShB, an RNA-sequencing analysis was performed using Rhizoctonia solani AG1-IA-inoculated rice leaves. Results: After 48 hours of inoculation, 6,838 genes were differentially expressed in rice leaves (>2 fold, P<0.05). Among them, 3,802 genes were upregulated, while 3,036 were downregulated compared to the control group. In addition, the differentially expressed genes were classified via GO, KEGG, and Mapman analyses. Thirty GO terms, including biological process, molecular function, and cellular component, were significantly enriched, and 30 KEGG pathways included ribosome, carbon metabolism, and biosynthesis of amino acids. A Mapman analysis demonstrated that the phytohormone and metabolic pathways were significantly altered. Interestingly, the expression levels of 359 transcription factors, including WRKY, MYB, and NAC family members, as well as 239 transporter genes, including ABC, MFS, and SWEET, were significantly changed upon R. solani AG1-IA inoculation. An additional genetic study showed that OsWRKY53 negatively and OsAKT1 positively regulate rice defense to R. solani, respectively. In addition, interestingly, many differentially expressed genes contain R. solani-responsive cis-elements in their promoter region. Conclusions: Taken together, our analyses provide valuable information for the additional study of rice defense mechanisms to ShB, and the genes identified could be useful in the future to breed resistant rice.

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Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize

Cited by 11 publications

References 49 publications

Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

Transcriptome analysis of rice leaves in response to Rhizoctonia solani infection

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