Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Xue, Mande; Long, Yan; Zhao, Zhiqiang; Huang, Gege; Huang, Ke; Zhang, Tianbao; Jiang, Ying; Yuan, Qianhua; Pei, Xinwu

doi:10.3390/ijms19072009

Cited by 22 publications

(15 citation statements)

References 42 publications

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“…Given the fact that there are few studies on the tissue‐specific synthetic promoter in rice, let alone with bidirectional driving activities, we reviewed the green tissue‐specific expression regulatory sequences obtained in recent years as a repository available for future researchers (Figure ). Considering that the length of regulatory sequence is closely related to the usability and flexibility of synthetic promoters, the length, specificity and expression efficiency of cis ‐regulatory elements were taken into account (Cai et al ., ; Huang, ; Lin et al ., ; Thilmony et al ., ; Xu et al ., ; Yang et al ., ). Furthermore, it should be paid particular attention that cis ‐regulatory elements assembled in synthetic promoters always bring inconsistent function with earlier reports (Wang et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…In the process of constructing synthetic promoters, these factors should be concerned with different degrees to meet individual demands. Consequently, we selected ten expression regulatory sequences based on a statistical results of relevant existing reports (Cai et al ., ; Carlos et al ., ; Gianì et al ., ; Huang, ; Jeong and Jung, ; Kuwano et al ., ; Lin et al ., ; Lu et al ., ; Manikandan et al ., ; Molla et al ., ; Nguyen et al ., ; Park et al ., ; Wang et al ., ; Xu et al ., ; Yang et al ., ; Zhang et al ., ). The four features of each expression regulatory sequence (tissue specificity, expression efficiency, sequence length and universality) were, respectively, scored based on our scoring standard, as shown in Table S1 in detail.…”

Section: Methodsmentioning

confidence: 99%

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Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Bai

Wang

et al. 2019

Plant Biotechnology Journal

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Summary Bidirectional green tissue‐specific promoters have important application prospects in genetic engineering and crop genetic improvement. However, there is no report on the application of them, mainly due to undiscovered natural bidirectional green tissue‐specific promoters and the lack of a comprehensive approach for the synthesis of these promoters. In order to compensate for this vacancy, the present study reports a novel strategy for the expression regulatory sequence selection and the bidirectional green tissue‐specific synthetic promoter construction. Based on this strategy, seven promoters were synthesized and introduced into rice by agrobacterium‐mediated transformation. The functional identification of these synthetic promoters was performed by the expression pattern of GFP and GUS reporter genes in two reverse directions in transgenic rice. The results indicated that all the synthetic promoters possessed bidirectional expression activities in transgenic rice, and four synthetic promoters (BiGSSP2, BiGSSP3, BiGSSP6, BiGSSP7) showed highly bidirectional expression efficiencies specifically in green tissues (leaf, sheath, panicle, stem), which could be widely applied to agricultural biotechnology. Our study provided a feasible strategy for the construction of synthetic promoters, and we successfully created four bidirectional green tissue‐specific synthetic promoters. It is the first report on bidirectional green tissue‐specific promoters that could be efficiently applied in genetic engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Bai

Wang

et al. 2019

Plant Biotechnology Journal

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“…Recently, tissue-specific promoters have become the focus of plant genetic engineering. Researchers have isolated, cloned, and studied many tissue-specific promoters from different plants (Guo et al, 2010; Koehorst-van Putten et al, 2012; Chen et al, 2013; Zhu et al, 2014; Liu et al, 2015; Xue et al, 2018). The third type of promoter, the inducible promoter, is activated by abiotic or biotic stresses or hormone induction.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of PsDREB2 promoter involved in tissue-specific expression and abiotic stress response from Paeonia suffruticosa

Liu

Zhu

Tan

et al. 2019

PeerJ

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Dehydration-responsive element-binding factor 2 (DREB2) belongs to the C-repeat-binding factor (CBF)/DREB subfamily of proteins. In this study, a 2,245 bp PsDREB2 promoter fragment was isolated from the genome of Paeonia suffruticosa. The fragment was rich in A/T bases and contained TATA box sequences, abscisic acid (ABA)-response elements, and other cis-elements, such as MYB and CAAT box. The promoter was fused with the β-glucuronidase (GUS) reporter gene to generate an expression vector. Arabidopsis thaliana was transformed with a flower dipping method. Gus activity in different tissues and organs of transgenic plants was determined via histochemical staining and quantified via GUS fluorescence. The activity of promoter regulatory elements in transgenic plants under drought, low-temperature, high-salt, and ABA stresses was analyzed. The results showed that the PsDREB2 gene promoter was expressed in the roots, stems, leaves, flowers, and silique pods but not in the seeds of transgenic Arabidopsis. Furthermore, the promoter was induced by drought, low temperature, high salt, and ABA. Hence, the PsDREB2 promoter is tissue- and stress-specific and can be used in the genetic engineering of novel peony cultivars in the future.

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“…Functional studies of intergenic regions have attracted the most attention of researchers [ 11 ], and the role of noncoding DNA in phenotypic evolution has been reported many times in the literature [ 25 ]. The orientation of flanking genes may influence the evolution of intergenic regions, in which cis-regulatory elements are likely to be located [ 26 , 27 ]. The head-to-head clustering of genes in which two adjacent genes are separated by a short intergenic distance have been determined by the direction of gene expression in different transcription configurations, and are also prevalent and conserved in many eukaryotes, including yeasts, plants, invertebrates, and vertebrates [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Structural and Functional Analysis of a Bidirectional Promoter from Gossypium hirsutum in Arabidopsis

Jing

Wang

Hasi

et al. 2018

IJMS

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Stacked traits have become an important trend in the current development of genomically modified crops. The bidirectional promoter can not only prevent the co-suppression of multigene expression, but also increase the efficiency of the cultivation of transgenic plants with multigenes. In Gossypium hirsutum, Ghrack1 and Ghuhrf1 are head-to-head gene pairs located on chromosome D09. We cloned the 1429-bp intergenic region between the Ghrack1 and Ghuhrf1 genes from Gossypium hirsutum. The cloned DNA fragment GhZU had the characteristics of a bidirectional promoter, with 38.7% G+C content, three CpG islands and no TATA-box. Using gfp and gus as reporter genes, a series of expression vectors were constructed into young leaves of tobacco. The histochemical GUS (Beta-glucuronidase) assay and GFP (green fluorescence protein) detection results indicated that GhZU could drive the expression of the reporter genes gus and gfp simultaneously in both orientations. Furthermore, we transformed the expression vectors into Arabidopsis and found that GUS was concentrated at vigorous growth sites, such as the leaf tip, the base of the leaves and pod, and the stigma. GFP was also mainly expressed in the epidermis of young leaves. In summary, we determined that the intergenic region GhZU was an orientation-dependent bidirectional promoter, and this is the first report on the bidirectional promoter from Gossypium hirsutum. Our findings in this study are likely to enhance understanding on the regulatory mechanisms of plant bidirectional promoters.

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Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (Oryza rufipogon Griff.)

Cited by 22 publications

References 42 publications

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Comprehensive construction strategy of bidirectional green tissue‐specific synthetic promoters

Identification and characterization of PsDREB2 promoter involved in tissue-specific expression and abiotic stress response from Paeonia suffruticosa

Structural and Functional Analysis of a Bidirectional Promoter from Gossypium hirsutum in Arabidopsis

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