Functional analysis of Pcpme6 from oomycete plant pathogen Phytophthora capsici

Feng, Baozhen; Li, Peiqian; Wang, Hemei; Zhang, Xiuguo

doi:10.1016/j.micpath.2010.03.004

Cited by 26 publications

(23 citation statements)

References 39 publications

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“…The prevalence of Phytophthora blight has increased worldwide infecting crowns, stems, roots, fruits and foliar parts of plant (Oelke et al, 2003;Hausbeck, Lamour, 2004;Bosland, 2008;QuesadaOcampo et al, 2011). A PCPME6 gene encoding PME is responsible for pathogenesis of P. capsici on pepper (Feng et al, 2010). The reproductive part of P. capsici is called sporangium containing spores (antheridium or oogonium) by which further zoospores are formed (Fig.…”

Section: Epidemiology and Disease Cycle Of Causal Agent Phytophthora mentioning

confidence: 99%

“…Manipulation of resistant genes has become very common for producing disease resistant crops with better yield (Ali et al, 2014 b). A PCPME6 gene encoding pectin methylesterase (PME) is considered to be responsible for the pathogenesis of P. capsici on pepper (Feng et al, 2010). Genetic control of disease is an efficient, long term, competitive, cost effective and environment friendly way to control pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Phytophthora capsici on chilli pepper (Capsicum annuum L.) and its management through genetic and bio-control: a review

Majid

Awan

Fatima³

et al. 2016

Zemdirbyste-Agriculture

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Phytophthora capsici is the most destructive pathogen of vegetables that represents a serious threat to chilli pepper plants. We discussed the control of P. capsici through manipulation of genetic architecture of chilli plant and endophytic microorganisms. The function of various genes encoding transcriptional regulatory and defense related putative proteins such as pathogen-related protein (PR), anti-microbial peptides (AMPs), polygalacturonaseinhibiting proteins (PGIPs), lipid transfer protein (LTP), pectin methylesterase (PME), leucine-rich repeat proteins (LRRs), osmotin-like and thaumatin-like protein, in Capsicum was also analyzed. The bio-control of P. capsici by using various strains of Bacillus, Trichoderma, Pseudomonas, Chryseobacterium and Rhizobacteria was demonstrated. We also discussed the enhanced resistance to P. capsici infection by treatment with a variety of abiotic and biotic inducers that act on defence signalling pathways involved in disease resistance. We highlighted the vulnerability of chilli crop with reference to its genetic resources against Phytophthora blight. Disease control through chemicals is becoming problematic, so we proposed other ways to control the disease severity. This review highlights the economic significance of chilli pepper (Capsicum annuum L.) along with disease management strategies against P. capsici. This pathogen has posed a serious threat to chilli crop worldwide.

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Section: Epidemiology and Disease Cycle Of Causal Agent Phytophthora mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytophthora capsici on chilli pepper (Capsicum annuum L.) and its management through genetic and bio-control: a review

Majid

Awan

Fatima³

et al. 2016

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

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“…To isolate the pathogen, we treated diseased plant tissues with 70% ethanol for 30-50 s and then soaked them in 0.1% mercuric chloride for 5 min, rinsed them in sterilized distilled water twice, and plated them on 10% V8 plate at 28°C (Feng et al, 2010). Single-zoospore isolates were derived from hyphae grown on plates and subcultured on V8 plates for 7 days in darkness.…”

Section: P Capsici Strainmentioning

confidence: 99%

“…Seedlings were grown as previously described (Feng et al, 2010). P. capsici sporangia and zoospores were produced as previously described (Feng et al, 2010).…”

Section: Production Of Infected Pepper Leaf Tissuementioning

confidence: 99%

“…Seedlings were grown as previously described (Feng et al, 2010). P. capsici sporangia and zoospores were produced as previously described (Feng et al, 2010). Detached leaves obtained during the 5th and 6th leaf stage were inoculated with 2.5 μL zoospore suspension (1 x 10 5 zoospores/mL) and placed on 1.5% (w/v) water agar plates in darkness for 7 days at 28°C.…”

Section: Production Of Infected Pepper Leaf Tissuementioning

confidence: 99%

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Molecular characterization and functional analysis of the Nep1-like protein-encoding gene from Phytophthora capsici

Feng

Li²

2013

Genet. Mol. Res.

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ABSTRACT. Phytophthora capsici is an aggressive plant pathogen that affects solanaceous and cucurbitaceous hosts. Nep1-like proteins (NLPs) are a group of effectors found particularly in oomycetes and considered important virulence factors. We identified an NLP gene (phcnlp1) from the highly virulent P. capsici strain Phyc12 that had an encoded polypeptide of 476-amino acid residues and a predicted molecular mass of 51.75 kDa. We performed quantitative reverse transcription-polymerase chain reaction to detect the expression pattern of phcnlp1 during various phases of interaction with the host plant, and the results showed that phcnlp1 was increasingly expressed during symptom development after P. capsici infection of pepper leaves. We also confirmed that phcnlp1 caused significant necrosis on tobacco plants when expressed based on potato virus agroinfection. All results indicated that phcnlp1 belongs to the NLP gene family and is important for the pathogenesis of P. capsici in its hosts.

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