Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay

Zahur, Muzna; Maqbool, Asma; Irfan, Muhammad; Barozai, Muhammad Younas Khan; Qaiser, Uzma; Rashid, Bushra; Husnain, Tayyab; Riazuddin, S.

doi:10.1007/s11033-008-9399-9

Cited by 26 publications

(18 citation statements)

References 36 publications

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“…However, because of the limited insight in signaling pathways that play a role in wheat-stripe rust interactions, it is unclear how TaHSC70 is regulated by JA. In another respect, the transcriptional regulation of genes enconding HSP is known to due to the activation of the correlative transcription factors, which bind to the HSP promoter elements [19,49]. Thereby mining these transcription factors and DNA elements could help to further investigate the biological functions of TaHSC70 in response to the environmental stress.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light-dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.

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Section: Discussionmentioning

confidence: 99%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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“…The W-box (TTGACC/T), found in F3 (-303) (-303 to -256 region), probably could have led to MeJA-(2.09-fold) and wound-inducible (3.05-fold) GUS expression. WRKY transcription factors have been reported to interact with the W-box in the transactivation of pathogen-or hormone-responsive (SA, MeJA) genes (Zahur et al 2009). Besides the W-box (TGAC), TGACG motif (MeJA responsive), Box E, and TCA elements (SA responsive) present between -843 and -613 region upstream of the transcription start site may also play important roles in the MeJA and wound induction of F7 (-843) construct to an extent of 4.14-and 3.81-fold, respectively (Fig.…”

Section: Phytohormone and Wound-induced Expression Analysismentioning

confidence: 99%

Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones

Gajjeraman

Prasad

2011

Plant Cell Rep

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The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) flanking the 5' ScMYBAS1 coding region from the sugarcane genome. A series of PScMYBAS1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -843, -1,033) was fused to the uidA reporter gene (GUS) and each deletion construct was analyzed by Agrobacterium-mediated transient transformation in tobacco leaves subjected to dehydration, salinity, cold, wounding, gibberellic acid (GA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Deletion analysis of the promoter, PScMYBAS1, suggested that the 303-bp promoter region was required for basal expression. Promoter fragments, 777 bp or longer showed ~twofold to ~fourfold increased induction of GUS in response to abiotic stress (dehydration, salt, cold, wounding) and hormone (SA, MeJA) treatments. These findings further our understanding of the regulation of ScMYBAS1 expression and provide a new stress-inducible promoter system in transgenic plants.

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“…The strongest evidence for the direct link between HSF, HSP expression, and thermotolerance comes from the demonstration that constitutive expression of HSF increased the level of thermotolerance in Arabidopsis [37]. Functional analysis of the cotton sHSP promoter in response to abiotic stresses has been shown to increase the thermotolerance in tobacco [44].…”

Section: Introductionmentioning

confidence: 99%

Ecotypic variation in chloroplast small heat-shock proteins and related thermotolerance in Chenopodium album

Shakeel

Haq

Heckathorn

et al. 2011

Plant Physiology and Biochemistry

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Functional analysis of cotton small heat shock protein promoter region in response to abiotic stresses in tobacco using Agrobacterium-mediated transient assay

Cited by 26 publications

References 36 publications

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones

Ecotypic variation in chloroplast small heat-shock proteins and related thermotolerance in Chenopodium album

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