Functional analysis of CfSnf1 in the development and pathogenicity of anthracnose fungus Colletotrichum fructicola on tea-oil tree

Zhang, Shengpei; Guo, Yuan; Li, Sizheng; Zhou, Guoying; Liu, Junang; Xu, Jianping; He, Lin

doi:10.1186/s12863-019-0796-y

Cited by 25 publications

(23 citation statements)

References 39 publications

(49 reference statements)

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“…Targeted CfGCN5 gene deletion was carried out by the one-step replacement strategy as per our previous description ( Zhang et al, 2019 ). The two ∼1.0 kb sequences flanking CfGCN5 were overlapped to the two sides of the HPH (hygromycin resistance cassette) gene, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…We recently demonstrated that CfSnf1, a key component of AMPK (AMP-activated protein kinase), regulates the utilization of specific carbon sources, appressorium formation, and pathogenicity in C. fructicola ( Zhang et al, 2019 ). In Saccharomyces cerevisiae , ScSnf1 cooperates with the histone acetyltransferase ScGcn5 (general control non-derepressible 5) to regulate transcription ( Lo et al, 2001 ; Abate et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

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The Histone Acetyltransferase CfGcn5 Regulates Growth, Development, and Pathogenicity in the Anthracnose Fungus Colletotrichum fructicola on the Tea-Oil Tree

et al. 2021

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The tea-oil tree (Camellia oleifera Abel.) is a commercial edible-oil tree in China, and anthracnose commonly occurs in its plantations, causing great losses annually. We have previously revealed that CfSnf1 is essential for pathogenicity in Colletotrichum fructicola, the major pathogen of anthracnose on the tea-oil tree. Here, we identified CfGcn5 as the homolog of yeast histone acetyltransferase ScGcn5, which cooperates with ScSnf1 to modify histone H3 in Saccharomyces cerevisiae. Targeted gene deletion revealed that CfGcn5 is important in fungi growth, conidiation, and responses to environmental stresses. Pathogenicity assays indicated that CfGcn5 is essential for C. fructicola virulence both in unwounded and wounded tea-oil tree leaves. Further, we found that CfGcn5 is localized to the nucleus and this specific localization is dependent on both NLS region and HAT domain. Moreover, we provided evidence showing that the nuclear localization is essential but not sufficient for the full function of CfGcn5, and the NLS, HAT, and Bromo domains were proven to be important for normal CfGcn5 functions. Taken together, our studies not only illustrate the key functions of CfGcn5 in growth, development, and pathogenicity but also highlight the relationship between its locations with functions in C. fructicola.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Histone Acetyltransferase CfGcn5 Regulates Growth, Development, and Pathogenicity in the Anthracnose Fungus Colletotrichum fructicola on the Tea-Oil Tree

et al. 2021

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“…oleifera anthracnose. We recently demonstrated that CfSnf1, CfSte50, and CfGcn5 were involved in pathogenicity by regulating the appressorium formation in C. fructicola ( Zhang et al, 2019 , 2021 ; Chen et al, 2020 ). Transcription factor of CfSte12 and the MAP Kinase CfPmk1 are key regulators of pathogenesis of C. fructicola ( Liang et al, 2019 ; Liu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The SNARE Protein CfVam7 Is Required for Growth, Endoplasmic Reticulum Stress Response, and Pathogenicity of Colletotrichum fructicola

Zhang

et al. 2021

Front. Microbiol.

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The tea-oil tree Camellia oleifera is native to China and is cultivated in many parts of southern China. This plant has been grown for over 2,000 years, mainly for its high-quality cooking oil. Anthracnose is the main disease of tea-oil tree and results in a huge loss annually. Colletotrichum fructicola is a major pathogen causing anthracnose on tea-oil tree. In a previous study, we characterized that the bZIP transcription factor CfHac1 controlled the development and pathogenicity of C. fructicola. Here, we identified and characterized the function of CfVAM7 gene, which was significantly downregulated at the transcriptional level in the ΔCfhac1 strain under dithiothreitol stress. Targeted gene deletion revealed that CfVam7 is important in growth, pathogenicity, and responses to endoplasmic reticulum-related stresses. Further analysis revealed that CfVam7 is required for appressorium formation and homotypic vacuole fusion, which are important for fungal pathogen invasion. Cytological examinations revealed that CfVam7 is localized to vacuole membranes in the hyphal stage. The Phox homology (PX) and SNARE domains of CfVam7 were indispensable for normal cellular localization and biological function. Taken together, our results suggested that CfVam7-mediated vacuole membrane fusion promotes growth, stress response, and pathogenicity of C. fructicola.

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“…fructicola were studied. Previously, we had shown that CfSNF1 , CfSTE50 , CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019, 2020; Yao et al, 2019; Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C .…”

Section: Introductionmentioning

confidence: 99%

“…In this preliminary study, the functions of some genes of C. fructicola were studied. Previously, we had shown that CfSNF1, CfSTE50, CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019(Chen et al, , 2020Yao et al, 2019;Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C. fructicola and found that the effector molecule inhibits the immune response of plants in the early stage of infection (Shang et al, 2020).…”

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A HOPS protein, CfVps39, is required for appressorium formation, environmental stress response and vacuolar fusion of Colletotrichum fructicola

Zhang

2021

Forest Pathology

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The tea‐oil tree Camellia oleifera is native to China and is cultivated in many parts of southern China. Anthracnose on Ca. oleifera is one of the most serious diseases in China. The major causal pathogen of tea‐oil anthracnose is Colletotrichum fructicola, but the detailed molecular mechanisms of its pathogenicity are still largely unclear. Vacuolar protein sorting 39 (Vps39) is a component of the homotypic fusion and vacuole protein sorting (HOPS) complex. To investigate the role of HOPS proteins in the fungal plant anthracnose pathogen C. fructicola, the CfVPS39 gene was deleted. This resulted in reduced mycelial growth, conidial production and complete loss of pathogenicity on tea‐oil leaves and fruits. In addition, ∆Cfvps39 showed increased sensitivity to cell wall stress, osmotic stress and endoplasmic reticulum stress. Further analysis revealed that CfVps39 is required for appressorium formation and homotypic vacuole fusion, both important for fungal pathogen invasion. Our study provides evidence that CfVps39 has a role in conidiation, stress response, appressorium formation, homotypic vacuole fusion and virulence against tea‐oil. This research not only provides the theories for revealing the pathogenic molecular mechanism of C. fructicola, but also has important guiding significance to reveal new fungicide targets to control this devastating disease.

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Functional analysis of CfSnf1 in the development and pathogenicity of anthracnose fungus Colletotrichum fructicola on tea-oil tree

Cited by 25 publications

References 39 publications

The Histone Acetyltransferase CfGcn5 Regulates Growth, Development, and Pathogenicity in the Anthracnose Fungus Colletotrichum fructicola on the Tea-Oil Tree

The Histone Acetyltransferase CfGcn5 Regulates Growth, Development, and Pathogenicity in the Anthracnose Fungus Colletotrichum fructicola on the Tea-Oil Tree

The SNARE Protein CfVam7 Is Required for Growth, Endoplasmic Reticulum Stress Response, and Pathogenicity of Colletotrichum fructicola

A HOPS protein, CfVps39, is required for appressorium formation, environmental stress response and vacuolar fusion of Colletotrichum fructicola

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