Functional analysis of a chaetoglobosin A biosynthetic regulator in Chaetomium globosum

CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum

“…4 ), indicated that CgVeA played an important regulatory role in the biosynthesis of cheA by controlling the transcription levels of the core enzymes in the gene cluster. Furthermore, the results showed that effectively enhanced expression of CgVeA had a positive impact on the expression levels of LaeA and cheR , which were identified to be positively correlated with cheA biosynthesis [ 13 ], especially under light condition. In contrast, both LaeA and cheR were obviously reduced in PS ( Fig.…”

“…In the past 5 years, our research group has progressively explored the factors influencing cheA production. CgLaeA and CgcheR, the Zn(II) 2 Cys 6 structural and pathway-specific transcription factors, respectively, have been identified in C. globosum , and have been found to exert positive effects (especially, CgcheR ) on target compounds synthesis [ 13 , 33 ]. Furthermore, EMSA results have revealed that CgcheR shared a relatively strong shift signal to PKS gene, which has been reported to be associated with polymerization of monomeric substance to form the carbon backbone [ 12 ].…”

“…By using an RNA-mediated gene silencing strategy, the gene cluster involved in cheA biosynthesis, which is most similar to the aspyridone gene cluster in Aspergillus nidulans , has been identified in Penicillium expansum [ 11 ]. Bioinformatics analysis has revealed that the cheA biosynthetic gene cluster consists of seven elements, such as the core region PKS-NRPS, a trans enoyl-reductase (ER), an FAD-dependent monooxygenase (FMO), two putative p450 monooxygenases, and two putative C6 transcription factors [ 12 , 13 ]. PKS-NRPS plays a major role in the condensation of acetate/malonate to form the carbon skeleton in the biosynthesis of chaetoglobosins [ 14 ].…”

“…Over the past 5 years, our research team has attempted to identify and study the function of regulators that play an essential role in morphological development and cheA biosynthesis in C. globosum . The pathway-specific regulator CgcheR , which has an essential role in modulating sporulation and cheA production, was identified via bioinformatics analysis in C. globosum [ 13 ]. Disruption of CgcheR caused a significant decrease in the expression of genes associated with cheA biosynthetic gene cluster, and the CgcheR disruptant lost the ability to produce spores.…”

Deletion of a Rare Fungal PKS CgPKS11 Promotes Chaetoglobosin A Biosynthesis, Yet Defers the Growth and Development of Chaetomium globosum

“…After a spontaneous intramolecular condensation and a Diels-Alder reaction, the key intermediate prochaetoglobosin is generated, which can be furnished into ChA after a set of oxidative modifications. Through gene disruption, homologs of CheA (CgcheA with a gene locus CHGG_01239) and CheB (CgcheB with a gene locus CHGG_01238) as well as the enzymes CheD (CgcheE with a gene locus CHGG_01242-1), CheE (CgcheF with a gene locus CHGG_01242-2), and CheG (CgcheG with a gene locus CHGG_01243), involved in the final oxidative transformations, are identified and characterized in C. globosum [17,18]. Nonetheless, we previously observed in C. globosum NK102 that a putative pigment polyketide synthase gene, pks-1/alb1, was also required for ChA biosynthesis [19].…”