CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum

Wang, Zhengran; Zhao, Shanshan; Zhang, Kai; Lin, Congyu; Ru, Xin; Yang, Qian

doi:10.1016/j.synbio.2022.07.002

Cited by 3 publications

(3 citation statements)

References 47 publications

(54 reference statements)

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“…In contrast, transcript levels of the CgVeA gene in samples grown under illumination increased considerably ( Figure 3 E). Similar to existing research findings [ 28 , 29 ], the results suggest that brlA could have a positive regulatory role in conidial formation, whereas CgVeA could have a negative role in the process. A low expression level of brlA was observed in the CgXpp1 -N14 disruptive mutant relative to the controls; however, the transcript level of the CgVeA gene increased significantly, which could be associated with the relatively low spore production in the transformants.…”

Section: Resultssupporting

confidence: 90%

“…To elucidate the effect of CgXpp1 on C. globosum development, the brlA gene, which has been identified as a critical gene for conidiophore formation in Acremonium chrysogenum [ 28 ], was examined by qRT-PCR. The expression of the CgVeA gene, which encodes a light-dependent regulator necessary for sporulation, was also detected in all species [ 29 , 30 ]. The expression of the brlA gene in all fungal strains tested under dark conditions was higher than that in strains incubated under light conditions ( Figure 3 D).…”

Section: Resultsmentioning

confidence: 99%

“…Electrophoretic mobility shift assays were carried out as previously described by Wang et al and Li et al [ 29 , 59 ]. The DNA probes containing the promoter regions (500-bp: from −400 to +100) of CgPKS , CgP450 , CgFMO , CgER , brlA , CgVeA , CgLaeA , and CgcheR , were PCR-amplified using the primers presented in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

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Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Zhao

Zhang

Lin

et al. 2022

IJMS

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Chaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by Chaetomium globosum. However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered its application and commercialization in agriculture and biomedicine. Here, a comprehensive study of the CgXpp1 gene, which encodes a basic helix-loop-helix family regulator with a putative role in the regulation of fungal growth and CheA biosynthesis, was performed by employing CgXpp1-disruption and CgXpp1-complementation strategies in the biocontrol species C. globosum. The results suggest that the CgXpp1 gene could be an indirect negative regulator in CheA production. Interestingly, knockout of CgXpp1 considerably increased the transcription levels of key genes and related regulatory factors associated with the CheA biosynthetic. Disruption of CgXpp1 led to a significant reduction in spore production and attenuation of cell development, which was consistent with metabolome analysis results. Taken together, an in-depth analysis of pleiotropic regulation influenced by transcription factors could provide insights into the unexplored metabolic mechanisms associated with primary and secondary metabolite production.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Zhao

Zhang

Lin

et al. 2022

IJMS

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Discovery and enantioselective total synthesis of antitumor agent asperfilasin A via a regio- and diastereoselective Nazarov cyclization

Wang,

Qi,

Chen

et al. 2024

Chinese Chemical Letters

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Involvement of LaeA and Velvet Proteins in Regulating the Production of Mycotoxins and Other Fungal Secondary Metabolites

Hou,

Liu,

et al. 2024

JoF

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Fungi are rich sources of secondary metabolites of agrochemical, pharmaceutical, and food importance, such as mycotoxins, antibiotics, and antitumor agents. Secondary metabolites play vital roles in fungal pathogenesis, growth and development, oxidative status modulation, and adaptation/resistance to various environmental stresses. LaeA contains an S-adenosylmethionine binding site and displays methyltransferase activity. The members of velvet proteins include VeA, VelB, VelC, VelD and VosA for each member with a velvet domain. LaeA and velvet proteins can form multimeric complexes such as VosA-VelB and VelB-VeA-LaeA. They belong to global regulators and are mainly impacted by light. One of their most important functions is to regulate gene expressions that are responsible for secondary metabolite biosynthesis. The aim of this mini-review is to represent the newest cognition of the biosynthetic regulation of mycotoxins and other fungal secondary metabolites by LaeA and velvet proteins. In most cases, LaeA and velvet proteins positively regulate production of fungal secondary metabolites. The regulated fungal species mainly belong to the toxigenic fungi from the genera of Alternaria, Aspergillus, Botrytis, Fusarium, Magnaporthe, Monascus, and Penicillium for the production of mycotoxins. We can control secondary metabolite production to inhibit the production of harmful mycotoxins while promoting the production of useful metabolites by global regulation of LaeA and velvet proteins in fungi. Furthermore, the regulation by LaeA and velvet proteins should be a practical strategy in activating silent biosynthetic gene clusters (BGCs) in fungi to obtain previously undiscovered metabolites.

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CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum

Cited by 3 publications

References 47 publications

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Discovery and enantioselective total synthesis of antitumor agent asperfilasin A via a regio- and diastereoselective Nazarov cyclization

Involvement of LaeA and Velvet Proteins in Regulating the Production of Mycotoxins and Other Fungal Secondary Metabolites

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