Functional analyses of nanoparticle toxicity: A comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum)

Song, Uhram; Jun, Heeju; Waldman, Bruce; Roh, Jinkyu; Kim, Younghun; Yi, Jongheop; Lee, Eun Ju

doi:10.1016/j.ecoenv.2013.03.033

Cited by 323 publications

(129 citation statements)

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“…SOD is the first line of the defense mechanism and scavenges superoxide radicals (Bowler et al, 1992;Fatima and Ahmad, 2005). An increase in SOD activity of L. esculentum was reported in AgNPs treated plants (Song et al, 2013). In Ricinus communis, AgNPs treatment enhanced SOD activity up to 1.000 mg/L application; however, it was decreased in 2.000 mg/L AgNPs-treated plants (Yasur and Rani, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…We also analyzed total chlorophyll content to evaluate nanotoxicity. Previous studies demonstrated that AgNPs decreased total chlorophyll content in Arabidopsis thaliana (Qian et al, 2013), Lycopersicon esculentum (Song et al, 2013), Oryza sativa (Nair and Chung, 2014a), and Vigna radiata (Nair and Chung, 2015). These studies are parallel to our study; we found a remarkable decrease in total ISSR807 6 0 2 0 2 2 2 0 1 1 3 1 0 3 3 0 3 ISSR880 5 1 0 0 1 1 0 0 2 0 1 2 1 4 1 0 1 Total bands 43 41 38 18 46 a+b+c+d 22 31 36 46 chlorophyll content especially in high concentration AgNPs treatment, and this result could cause an inhibition in photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Nanoparticles could uncertainly spread to the environment. However, the interaction between AgNPs and plant systems is still not well known (Patlolla et al, 2012;Song et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Çekiç¹,

Ekinci²,

Inal³

et al. 2017

Turk J Biol

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IntroductionSilver nanoparticles (AgNPs) are widely used among other nanoparticles in many industries within a wide range of consumer products because of their antibacterial and biocidal properties (Thuesombat et al., 2014). In recent years, the significant increase in the consumption of nanoparticles has caused environmental, health, and safety concerns regarding their potential effects (Ma et al., 2010;Pokhrel and Dubey, 2013). Nanoparticles could uncertainly spread to the environment. However, the interaction between AgNPs and plant systems is still not well known (Patlolla et al., 2012;Song et al., 2013).AgNPs are known to be absorbed by plants and could interact with intracellular parts causing water imbalances, cell damage, and decreases in photosynthesis (Kumari et al., 2009;Qian et al., 2013). They are also reported to have genotoxic effects on plant cells, inducing chromosomal aberrations and micronucleus induction (Patlolla et al., 2012). However, the impacts of nanoparticles on plants can vary according to the nanoparticle concentration, size, chemical properties, and plant species (Ma et al., 2010;Thuesombat et al., 2014).Nanotoxicity could lead to oxidative stress and previous studies indicate that AgNPs could induce toxicity due to their effect on reactive oxygen species (ROS) formation (Qian et al., 2013;McShan et al., 2014). The imbalance of ROS production and antioxidant activity can cause oxidative damage, and plants cope with this oxidative damage by their antioxidant defense mechanism (Saed-Moucheshi et al., 2014). Previously, studies on the genotoxicity of nanoparticles have used cell viability, chromosome aberration, or micronucleus assays to identify the genotoxic effect, and comet analysis for detecting the DNA damage in different plant species (Kumari et al., 2009;Kumari et al., 2011;Patlolla et al., 2012;Ghosh et al., 2012). However, these methods are very restricted for identifying the genotoxic effects of nanoparticles at the DNA level. DNA-based techniques are sensitive and selective assays that help to determine the genotoxic effects of environmental pollutants on DNA. One of these methods used for these aims is the intersimple sequence repeat (ISSR)-PCR assay. ISSR-PCR uses as primer microsatellite repeats (Zietkiewicz et al., 1994). The ISSR-PCR method is more sensitive than the random amplified polymorphic DNA assay (RAPD) (Correia et al., 2014;Bajpai et al., 2015), because of the exhibiting specificity of the sequence-tagged-site markers and high ratio of reproducibility potential owing to the use of longer primers (16-25 bp).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Çekiç¹,

Ekinci²,

Inal³

et al. 2017

Turk J Biol

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“…The SNP treatments may cause toxicity to the plants. Song et al (2013) reported that in greenhouse experiments, mature tomato plants showed evidence of phytotoxicity due to AgNPs by exhibiting low chlorophyll contents, higher SOD, and less fruit production. Nevertheless, Darvishzadeh et al, (2015) found that the application of nano-silver particles at the concentration of 40 mg.kg-1 led to the increases in germination percentage and improved the resistance to salinity conditions in basil.…”

Section: Discussionmentioning

confidence: 99%

Effect of Silver Nanoparticles on Salt Tolerancy of Tomato Trans-plants (Solanum lycopersicom,L. Mill.)

2016

AJAS

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A pot experiment was conducted at the Experimental Farm, Faculty of Agriculture, Assiut University, Assiut, during the summer seasons of 2014 and 2015 to study the effect of Silver Nanoparticles (SNP) on salt tolerance in tomato. The experimental soil type was clay. Split plot arrangement in Randomized Complete Blocks Design (RCBD) with four replicates was used. The salt solution (sodium chloride) concentrations [(0 %( control plots were irrigated with deionized water), 30, 60, 90, 120 mM/L)] were distributed in main plots, while the Silver Nanoparticles concentrations [(0 %( control plots were soaked in distilled water), 10, 20, 40 ppm)] were arranged in sub plots. The obtained results indicated that soaking tomato transplants in Silver nanoparticles for 24 hours had no significant effect on increasing tomato tolerance to sodium chloride salt within the used levels. This was reflected in negative effects on fruit number per plant, fruit diameter, average fruit weight, number of branches per plant and plant height. The control treatment (soaked in distilled water) of SNP was consistently superior for all studied traits. SNP treatments reduced the fruit number per plant, fruit diameter, average fruit weight, number of branches per plant and plant height.

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“…A decrease in germination, a slowdown in the root nodules formation (due to a decreased number of the symbiotic bacteria Rhizobium leguminosarum) and shoot growth, and smaller root length in Vicia faba L. are described when Ag NPs were added to the culture medium [67). In hydroponic culture, the germination rate in Solanum lycopersicum [68] and Raphanus sativus L. did not decrease in the presence of Ag NPs, but root and shoot length decreased, and the photosynthetic activity was slightly reduced [69]. According to C.L.…”

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confidence: 99%

INTERACTIONS OF PLANTS WITH NOBLE METAL NANOPARTICLES (review)

Дыкман¹,

Shchyogolev²

2017

S-h. biol.

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A b s t r a c tGold and silver nanoparticles are used in a variety of biomedical practice as carriers of drugs, enhancers and/or converters of optical signal, immunomarkers, etc. The review examines a decade publications (2007)(2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016) pertaining to the various influence of nanoparticles of noble metals (gold and silver) on growth and productivity of higher plants. In fact, possible phytotoxicity of these nanoparticles is being actively studied for over 10 years. The topicality of this field of research is due to the detection of a number of natural and human-caused factors resulting in interactions of plants with nanoparticles (B.P. Colman et al., 2013; N.G. Khlebtsov et al., 2011). A positive or negative impact of nanoparticles on plants is little known, and the information is very contradictory (P. Manchikanti et al., 2010; M. Carrière et al., 2012; C. Remédios et al., 2012; N. Zuverza-Mena et al., 2016). In the study both model (Arabidopsis thaliana) and cultivated plants (soy, canola, beans, rice, radish, tomato, pumpkin, etc.) were involved. The discussed data are indicative of both positive and negative effects of metal nanoparticles on plants, as well as of the chemical nature, size, shape, surface charge, and the dose introduced being the major factors that are responsible for the processes of intracellular nanoparticle penetration. In general terms, it was mentioned that silver nanoparticles were more toxic as compared to gold ones being due to more active silver ion diffusion from the silver nanoparticle surface. Silver ions are known to inhibit effectively biosynthesis of ethylene -a phytohormone controlling processes of plant stress, aging etc., wherein gold ions do not influence ethylene biosynthesis and signaling. Considered all, metal ion toxicity exceeds considerably a toxicity of nanoparticles. The mechanism of the nanoparticle phytotoxic action is often connected with accumulation of active oxygen species in plant tissues. The use of cell suspension cultures may be a promising approach to study plant-nanoparticles interaction (E. Planchet et al., 2015). The period during which these studies are conducted is still small for elucidating all aspects with regard to biosafety. Contradictory (often conflicting) information on the impact of nanoparticles, in our opinion, is a result of diverse experimental conditions used. It is noted that while being clearly incomplete and contradictory, the obtained data suggest that a coordinated research program is needed that would detect correlations between particle parameters, experimental design, and the observed biological effects.Keywords: gold nanoparticles, silver nanoparticles, toxicity, biological effects, plantsIn recent decades, both scientists and the world community have paid much attention to nanotechnology, based on the use of objects no larger than 100 nm in the synthesis, assembly and modification of substances, materials and structures with unusual (often unexpected) properties. The specific chara...

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Functional analyses of nanoparticle toxicity: A comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum)

Cited by 323 publications

References 33 publications

Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Silver nanoparticles induced genotoxicity and oxidative stress in tomato plants

Effect of Silver Nanoparticles on Salt Tolerancy of Tomato Trans-plants (Solanum lycopersicom,L. Mill.)

INTERACTIONS OF PLANTS WITH NOBLE METAL NANOPARTICLES (review)

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