Functional activity of antibodies after immunization of Finnish and Israeli infants with an 11-valent pneumococcal conjugate vaccine

Wuorimaa, Tomi; Dagan, Ron; Bailleux, Fabrice; Haikala, Raili; Ekström, Nina; Eskola, Juhani; Yaich, Mansour; Käyhty, Helena

doi:10.1016/j.vaccine.2005.06.023

Cited by 19 publications

(10 citation statements)

References 16 publications

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“…This suggests better functional capacity of anti-6B than anti-19F antibodies. The results are in agreement with the previous studies, demonstrating low functional activity of anti-19F antibodies (6,32,34,43), and with the data of Jokinen et al (20), showing that a higher mean antibody concentration is needed for protection against serotype 19F than for 6B AOM after PCV vaccination. Serotypes 6B and 23F elicited similar antibody concentrations, yet the concentration required for 50% killing of serotype 23F was 2 to 10 times as high as for 6B, indicating again the superiority of anti-6B antibodies.…”

Section: Discussionsupporting

confidence: 93%

“…Serotypes 6B and 23F elicited similar antibody concentrations, yet the concentration required for 50% killing of serotype 23F was 2 to 10 times as high as for 6B, indicating again the superiority of anti-6B antibodies. The finding was unexpected, since previously published data for different PCVs (6,32,34,43) indicate equal functional activity of anti-6B and -23F antibodies. However, similar results have been presented by Nurkka et al for an 11-valent PnPD vaccine (29a).…”

Section: Discussionmentioning

confidence: 72%

“…We could, however, not find any correlation between the concentration required for 50% killing and the relative avidity of antibodies when the comparisons were made within a time point for each PCV group and serotype. Accordingly, in the study by Wuorimaa et al (43), no such correlation was found. However, relatively small numbers of samples obtained at the same age may have resulted in the relatively narrow variation of AIs, which, in turn, may have obstructed us from observing a truly existing correlation.…”

Section: Discussionmentioning

confidence: 82%

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Functional Antibodies Elicited by Two Heptavalent Pneumococcal Conjugate Vaccines in the Finnish Otitis Media Vaccine Trial

et al. 2007

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In the Finnish Otitis Media Vaccine Trial, the now-licensed pneumococcal conjugate vaccine containing polysaccharides conjugated to protein CRM 197 (PncCRM) and the experimental pneumococcal polysaccharide-meningococcal outer membrane protein complex conjugate vaccine (PncOMPC), showed similar efficacy profiles against acute otitis media despite different antibody concentrations in sera. We now report the opsonophagocytic activities (OPA) in these sera. OPA, antibody concentration, and avidity for serotypes 6B, 19F, and 23F were determined in sera of infants who received either pneumococcal conjugate (PCV) or control vaccine at 2, 4, and 6 months of age and either the homologous or pneumococcal polysaccharide vaccine at 12 months of age. OPA varied by vaccine and serotype. The majority of PCV recipients had positive OPA after the fourth dose, while OPA was undetectable in the control group. Coinciding with the efficacy data, the concentration of antibodies required for 50% killing was low for 6B and high for 19F for both PCVs. Contradictory to the efficacy data, PncOMPC induced lower functional capacity to 23F than PncCRM. OPA correlated with antibody concentration, while avidity and functional capacity of antibodies showed no correlation. The OPA data provide valuable additional information for serotype-specific differences in protection and when evaluating serotype-specific immunogenicity and should thus be considered when defining serological correlates of protection.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 82%

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Functional Antibodies Elicited by Two Heptavalent Pneumococcal Conjugate Vaccines in the Finnish Otitis Media Vaccine Trial

et al. 2007

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“…The ability of anticapsular antibodies to enhance opsonophagocytosis was assessed by analyzing the pneumococcal isolates with pooled sera from infants immunized in a previous study with an 11-valent pneumococcal conjugate vaccine (53). Each serum pool was collected from post-booster sera of 5 to 11 different children and contained low, medium, or high concentrations of serotype-specific antibodies.…”

Section: Methodsmentioning

confidence: 99%

The Capsular Serotype ofStreptococcus pneumoniaeIs More Important than the Genetic Background for Resistance to Complement

et al. 2010

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The polysaccharide capsule of Streptococcus pneumoniae inhibits phagocytic killing by innate immune mechanisms. Certain serotypes are associated with invasive disease while others with a nasopharyngeal carriage. The invasiveness of serotypes may partly be explained by ability to resist deposition of complement (C3) on the bacterial surface and consequent opsonophagocytic killing. In our previous studies, we observed that clinical isolates of serotypes 1 and 5, which are rarely detected in asymptomatic carriage, were resistant to complement deposition and opsonophagocytosis, whereas serotypes 6B and 23F, both common in carriage, were more sensitive to deposition of C3 and opsonophagocytic killing. However, presence of significant variation in C3 deposition between isolates of the same serotype indicated that factors other than the capsule also affect complement resistance. To distinguish the relative effect of the capsular serotype and other virulence factors on C3 deposition, we compared capsule-switched mutants prepared in genetic backgrounds of pneumococcal strains TIGR4, 603, and 618. Clinical isolates which had the same multilocus sequence type but expressed different serotypes were also compared. We found that the serotype had a significant impact on complement resistance and that the more resistant the strain was to complement, the higher was the concentration of polysaccharide-specific antibodies required for opsonophagocytic killing. Comparison of strains expressing the same capsular polysaccharides in the different genetic backgrounds and various capsular mutants of the same strain suggests that while the genotype affects complement resistance, the serotype is the most important determinant. Differences between serotypes were more significant than the differences between strains.

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“…In contrast, the GMCs of serotype-specific IgG required for 50% killing ranged 2-119 ng/ml in infant populations after CV vaccination [41][42][43]. Collectively, our present data suggest that opsonic function of serotype-specific IgG after CV vaccination is much decreased in Ugandan adults, irrespective of HIV infection, than those in infants.…”

Section: Resultsmentioning

confidence: 59%

Induction of opsonophagocytic killing activity with pneumococcal conjugate vaccine in human immunodeficiency virus-infected Ugandan adults

Chen

Ssali

Mulungi

et al. 2008

Vaccine

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The levels of IgG determined by ELISA may have limited relevance in human immunodeficiency virus (HIV)-infected adults because of nonfunctional antibodies. 58HIV-1-infected and 29 HIV-uninfected Ugandan adults were immunized with conjugate vaccine (CV) followed by polysaccharide vaccine (PV) after a two-month interval, and the opsonophagocytic killing (OPK) titers against serotype 4 or 14 pneumococcal strains as well as the levels of serotype-specific IgG in sera were determined.Significant increases were found in the OPK titers and IgG levels for both serotypes after CV vaccination irrespective of HIV status. Increases in IgG levels and OPK titers were largely dependent on the CD4 + cell counts, except for increases in the IgG levels for serotype 4. The proportions with serum OPK titer equal to or greater than 8 were 0-4.3% for serotype 4 and 26.7-42.9% for serotype 14 before vaccination, but the proportions increased up to 43.3-86.2% for serotype 4 and 63.3-96.6% for serotype 14 in all three groups 2 months after CV vaccination. The serum OPK titers remained at levels higher than the pre-vaccination level for at least 8 months after CV vaccination. A

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Functional activity of antibodies after immunization of Finnish and Israeli infants with an 11-valent pneumococcal conjugate vaccine

Cited by 19 publications

References 16 publications

Functional Antibodies Elicited by Two Heptavalent Pneumococcal Conjugate Vaccines in the Finnish Otitis Media Vaccine Trial

Functional Antibodies Elicited by Two Heptavalent Pneumococcal Conjugate Vaccines in the Finnish Otitis Media Vaccine Trial

The Capsular Serotype ofStreptococcus pneumoniaeIs More Important than the Genetic Background for Resistance to Complement

Induction of opsonophagocytic killing activity with pneumococcal conjugate vaccine in human immunodeficiency virus-infected Ugandan adults

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