Function of the Glyoxylate-condensing Enzymes I. Growth of
            <i>Escherichia coli</i>
            on
            <i>n</i>
            -Valeric Acid

Furmanski, Philip; Wegener, Warner S.; Reeves, Henry C.; Ajl, Samuel J.

doi:10.1128/jb.94.4.1075-1081.1967

Cited by 9 publications

(3 citation statements)

References 8 publications

(11 reference statements)

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“…Malate synthase is present in cells of strain JOB5 after growth on all substrates tested. This enzyme is widely distributed in nature and is present under many conditions of growth (2,6,25). Methylmalonyl CoA mutase was also present after growth of strain JOB5 on a variety of substrates, and the enzymes involved in this pathway of propionate utilization are reported to be constitutive in those organisms in which this pathway has been demonstrated (1).…”

Section: Discussionmentioning

confidence: 99%

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Vestal

Perry

1969

J Bacteriol

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The metabolism of propane and propionate by a soil isolate (Brevibacterium sp. strain JOB5) was investigated. The presence of isocitrate lyase in cells grown on isopropanol, acetate, or propane and the absence of this inducible enzyme in n-propanol-and propionate-grown cells suggested that propane is not metabolized via C-terminal oxidation. Methylmalonyl coenzyme A mutase and malate synthase are constitutive in this organism. The incorporation of "4CO2 into pyruvate accumulated during propionate utilization suggests that propionate is metabolized via the methylmalonyl-succinate pathway. These results were further substantiated by radiorespirometric studies with propionate-1-'4C,-2-14C, and-3_14C as substrate. Propane-2-14C was shown, by unlabeled competitor experiments, to be oxidized to acetone; acetone and isopropanol are oxidized in this organism to acetol. Cleavage of acetol to acetate and CO2 would yield the inducer for the isocitrate lyase present in propane-grown cells.

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Section: Discussionmentioning

confidence: 99%

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Vestal

Perry

1969

J Bacteriol

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“…During Growth on Valerate The mechanism by which growth on valerate results in the formation of a mutant population is not clearly understood. Mutagenesis on valerate does not occur simply by selection of a spontaneously occurring mutant; instead, it appears to involve the intermediate formation of a valerate-"adapted" population which exhibits a high frequency of conversion to the mutant cell type (46,209).…”

Section: Studies On the Mechanism Of Mutagenesismentioning

confidence: 99%

“…Secondary growth time (right ordinate) expresses the time required for the respective samples to initiate growth in valerate (0) and butyrate (0) media. From Furmanski et al (46). a function of valeric acid specifically, and, after incubation in valeric acid, the change is maintained as a stable characteristic, even when cells are subsequently subcultured in complex media.…”

Section: Studies On the Mechanism Of Mutagenesismentioning

confidence: 99%

Alternate pathways of metabolism of short-chain fatty acids.

Wegener¹,

Reeves²,

Rabin³

et al. 1968

Bacteriological Reviews

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Synthesis of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli

Yim

Lee

Chang

2000

Biotechnol. Bioeng.

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Several recombinant Escherichia coli strains, including XL1‐Blue, JM109, HB101, and DH5α harboring a stable high‐copynumber plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyalkanoate (PHA) biosynthesis genes were constructed. These recombinant strains were examined for their ability to synthesize and accumulate poly‐(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) [P(3HB‐co‐3HV)] copolymer from glucose and either propionate or valerate. All recombinant E. coli strains could synthesize the P(3HB‐co‐3HV) copolymer in the medium containing glucose and propionate. However, only the homopolymer poly‐(3‐hydroxybutyrate) [P(3HB)] was synthesized from glucose and valerate. The PHA concentration and the 3HV fraction could be increased by inducing with acetate and/or oleate. When supplemented with oleate, the 3HV fraction increased by fourfold compared with that obtained without induction. Induction with propionate resulted in lower PHA concentration due to the inhibitory effect, but an 3HV fraction of as high as 33.0% could be obtained. These results suggest that P(3HB‐co‐3HV) can be efficiently produced from propionate by recombinant E. coli by inducing with acetate, propionate, or oleate. © 1996 John Wiley & Sons, Inc.

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Function of the Glyoxylate-condensing Enzymes I. Growth of Escherichia coli on n -Valeric Acid

Cited by 9 publications

References 8 publications

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Alternate pathways of metabolism of short-chain fatty acids.

Synthesis of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant Escherichia coli

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