Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Vestal, J. Robie; Perry, Jerome J.

doi:10.1128/jb.99.1.216-221.1969

Cited by 85 publications

(35 citation statements)

References 28 publications

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“…Manometry. Production of 4CO2 from the metabolism of [1-'4C]acetate by 2.5 mg (dry weight) of propane-or hexadecane-grown cells was determined as previously described (24). Acetate (68 pmol) containing 2 iLCi of labeled substrate was added to initiate the reaction.…”

Section: Methodsmentioning

confidence: 99%

Regulation of fatty acid biosynthesis by hydrocarbon substrates in Mycobacterium convolutum

Ascenzi¹,

Vestal²

1979

J Bacteriol

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When Mycobacterium convolutum R22 was grown on the n-alkanes C13 through C16, the predominant fatty acids were of the same chain length as the growth substrate. Cells grown on C13 through C16 n-alkanes incorporated between 15 and 85 pmol of acetate per pg of lipid into the fatty acids, whereas acetate-or propanegrown cells incorporated 280 and 255 pmol of acetate per ,ug of lipid, respectively.In vivo experiments demonstrated that hexadecane, hexadecanoic acid, and hexadecanoylcoenzyme A (CoA) all inhibited de novo fatty acid synthesis. Hexadecanoyl-CoA was the most potent inhibitor. Hexadecane and hexadecanoic acid inhibited acetyl-CoA carboxylase by up to 37 and 39%, respectively, at 1 mM. Hexadecanoyl-CoA inhibited the enzyme activity by 65% at 50 uM. Cells that were grown on C14 through C1s n-alkanes had about 25 times less acetyl-CoA carboxylase activity than did cells grown on acetate or propane, suggesting repressed levels of the enzyme. Hexadecaneor pentadecane-grown cells were found to have 5 to 10 times more intracellular free fatty acid than cells grown on acetate, propane, or ethane.Bacterial and yeast cells growing on longchain n-alkanes (C14 through C18) as sole sources of carbon and energy contain a large proportion of fatty acids that are the same carbon chain length as the hydrocarbon substrate (6, 7, 9a, 14, 20, 23, 25). King and Perry (11) have demonstrated that in hexadecane-grown Mycobacterium vaccae, the predominant fatty acids tetradecanoic, hexadecanoic, and hexadecenoic acids were derived directly from the carbon skeleton of hexadecane. Similarly, n-octadecane served as the immediate precursor for tetradecanoic, hexadecanoic, hexadecenoic, octadecanoic, and octadecenoic acids. Makula and Finnerty (16) demonstrated that Acinetobacter HO1-N accu-

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Section: Methodsmentioning

confidence: 99%

Regulation of fatty acid biosynthesis by hydrocarbon substrates in Mycobacterium convolutum

Ascenzi¹,

Vestal²

1979

J Bacteriol

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“…Therefore the possibility should be considered that this acetol monooxygenase is also present in other bacteria which assimilate certain C 3 compounds via the glyoxylate cycle as, for example, Mycobacterium JOB-5 during growth on acetone [4,5].…”

Section: Conditionsmentioning

confidence: 99%

“…Furthermore, isocitrate lyase activity was induced in Mycobacterium JOB-5 during growth on propane, indicating that propane is metabolized via acetate. Propane degradation by this organism was suggested to proceed via isopropanol and acetone to acetol, followed by a cleavage reaction yielding a C 2 and C 1 unit [4]. The C~ unit is probably formaldehyde [5].…”

Section: Introductionmentioning

confidence: 99%

Acetol monooxygenase from Mycobacterium Py1 cleaves acetol into acetate and formaldehyde

Hartmans¹

1986

FEMS Microbiology Letters

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A novel acetol monooxygenase has been detected in Mycobacterium Py1. Extracts of 1,2‐propanediol‐grown cells oxidized acetol in an oxygen‐and NADPH‐consuming reaction. The initial oxidation product is probably hydroxymethyleneacetate, which spontaneously rearranges to acetate and formaldehyde. Acetol oxidation was inhibited by carbon monoxide, but not by 10 mM cyanide, indicating that a cytochrome P‐450 type oxygenase might be involved. The enzyme activity was only detected in 1,2‐propanediol‐ or acetol‐grown cells, suggesting that this acetol monooxygenase plays a role in the metabolism of 1,2‐propanediol by Mycobacterium Py1.

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“…Figure 2 (lanes 4 and 5) also shows the LMW RNA profile of the strain JOB5 and the LMW RNA profile of type strain of Mycobacterium vaccae. Strain JOB5 was tentatively classified in this species using physiological criteria (PERRY, 1968;VESTAL and PERRY, 1969). The 5S rRNA zone profiles support the classification of this strain in the genus Mycobacterium, but its tRNA profile indicates that this strain does not belong to species M. vaccae.…”

mentioning

confidence: 74%

Analysis of Stable Low Molecular Weight (LMW) RNA Profiles of Hydrocarbon Metabolizing Bacteria by Staircase Electrophoresis

Trujillo

Velázquez

Mateos

et al. 2001

Systematic and Applied Microbiology

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Divergent Metabolic Pathways for Propane and Propionate Utilization by a Soil Isolate

Cited by 85 publications

References 28 publications

Regulation of fatty acid biosynthesis by hydrocarbon substrates in Mycobacterium convolutum

Regulation of fatty acid biosynthesis by hydrocarbon substrates in Mycobacterium convolutum

Acetol monooxygenase from Mycobacterium Py1 cleaves acetol into acetate and formaldehyde

Analysis of Stable Low Molecular Weight (LMW) RNA Profiles of Hydrocarbon Metabolizing Bacteria by Staircase Electrophoresis

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