1986
DOI: 10.1016/0378-1097(86)90303-4
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Acetol monooxygenase from Mycobacterium Py1 cleaves acetol into acetate and formaldehyde

Abstract: A novel acetol monooxygenase has been detected in Mycobacterium Py1. Extracts of 1,2‐propanediol‐grown cells oxidized acetol in an oxygen‐and NADPH‐consuming reaction. The initial oxidation product is probably hydroxymethyleneacetate, which spontaneously rearranges to acetate and formaldehyde. Acetol oxidation was inhibited by carbon monoxide, but not by 10 mM cyanide, indicating that a cytochrome P‐450 type oxygenase might be involved. The enzyme activity was only detected in 1,2‐propanediol‐ or acetol‐grown … Show more

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Cited by 12 publications
(15 citation statements)
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“…On the basis of the biochemical properties of acetol monooxygenase from Mycobacterium sp. strain Py1, this enzyme appears to be a BVMO (20). Therefore, it may be noteworthy to point out that two BVMOs are involved in two distinct catabolic pathways for acetone.…”
Section: Discussionmentioning
confidence: 91%
“…On the basis of the biochemical properties of acetol monooxygenase from Mycobacterium sp. strain Py1, this enzyme appears to be a BVMO (20). Therefore, it may be noteworthy to point out that two BVMOs are involved in two distinct catabolic pathways for acetone.…”
Section: Discussionmentioning
confidence: 91%
“…2H20. Maintenance of strains, culture harvesting and storage of harvested cells were as previously described (Hartmans & de Bont, 1986). …”
Section: Methodsmentioning
confidence: 99%
“…Crude cell extracts were prepared as described previously (Hartmans & de Bont, 1986) except that 10% (v/v) glycerol (87 %) was added before sonication. All chromatographic steps were done at 4 "C. Dialysis of crude extracts was done overnight at 4 "C against 200 vols 50 mM-potassium phosphate buffer, pH 7.3, with 8.7% glycerol (buffer A).…”
Section: Preparation Of Cell Extractsmentioning
confidence: 99%
“…When CAA was used as a carbon source it was sterilized separately by filtration and added at a concentration of 5 mM. Culture conditions, maintenance of strains, culture harvesting and storage of harvested cells were as previously described (Hartmans & de Bont, 1986). …”
Section: Introductionmentioning
confidence: 99%
“…Crude cell extracts and dialysed extracts were prepared as described previously (Hartmans & de Bont, 1986). …”
Section: Introductionmentioning
confidence: 99%