Function of laccase in the white-rot fungus Fomes annosus

Haars, A.; Hüttermann, Aloys

doi:10.1007/bf00446882

Cited by 67 publications

(21 citation statements)

References 13 publications

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“…annosum . This is in good agreement with results of Haars and Huttermann (1980) who reported that lignosulfonate was efficiently depolymerized by H . annosum even when laccase was completely inhibited by the copper‐chelating agent thioglycolic acid.…”

Section: Discussionsupporting

confidence: 93%

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Ihssen

Schubert²,

Schwarze³

et al. 2011

Journal of Applied Microbiology

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Aims: Wild‐type white rot fungi are the most important production organisms for laccase, a promising oxidative biocatalyst with numerous applications. This study aimed at identifying novel highly productive strains, finding optimal cultivation conditions for laccase production and establishing a simple immobilization procedure. Methods and Results: By using a newly developed 96‐well microplate cultivation method, 23 species of white rot fungi, represented by 29 strains, were directly compared with regard to the amount of secreted laccase. Both, with glucose and spruce saw dust as growth substrate a Heterobasidion annosum strain and a Physisporinus vitreus strain were the most productive (730–2200 U l−1 of secreted laccase). Cultivation conditions for laccase production with H. annosum were optimized in larger‐scale liquid cultures. Aeration with a sparger lead to a 3·8‐fold increase in laccase activity when compared to nonaerated flask cultures. More than 3000 U l−1 laccase was produced in glucose medium supplemented with yeast extract and the inducer veratryl alcohol. Culture supernatant was incubated with short‐range ordered Al(OH)3 particles to directly immobilize and concentrate laccase by adsorption. Active laccase was recovered in 40% yield and the Al(OH)3‐adsorbed laccase was suitable for repeated decolourization of indigo carmine. Conclusions: Microplate cultivation allowed a large‐scale comparison of the capacity of different fungal species for laccase production. Laccase secretion of a highly productive H. annosum strain was found to vary strongly with different cultivation conditions. Adsorption to Al(OH)3 proved to be suitable as direct immobilization technique. Significance and Impact of the Study: The microplate screening method simplifies strain and medium development for laccase production. Two novel fungal strains suitable for laccase production were identified. Procedures for simple and efficient production of immobilized H. annosum laccase were established.

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Section: Discussionsupporting

confidence: 93%

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Ihssen

Schubert²,

Schwarze³

et al. 2011

Journal of Applied Microbiology

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“…The low water content might be affecting by increasing the water tension and decreasing the substrate swelling. The non-involvement of laccase in lignin degradation has also been indicated [27] whereas others wign it a dual role i.e. simultaneous condensation and depolymerization [28].…”

Section: Discussionmentioning

confidence: 99%

Degradation of lignocellulosic residues by polyporus versicolor and the effect of moisture contents and phenolic compounds

Arora

Sandhu

1986

Acta Biotechnologica

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Polyporus versicolor was selected to find out its ability to degrade four different lignocellulosic residues (angiospermic wood sawdust, sugarcane bagasse, paddy and wheat straw) under semisolid conditions. The production of laccase was also studied on these substrates. Sawdust suffered a maximum lignin loss though overall reduction in weight was maximum in paddy straw. Addition of malt extract and certain phenolic compounds (gallic acid, tannic acid and orcinol) favoured ligninolysis in sawdust. A moisture level of 5 ml/g of sawdust was found to be the most suitable for degradation whereas laccase yield increased with further rise in moisture content.

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“…Laccase activities in Heterobasidion have been described (Haars &Hüttermann 1980a, Daniel et al 1998, Al-Adhami et al 2002, Lang 2004 and genes for Mn-dependent peroxidases are known to exist in the genus (Maijala et al 2003). Laccases play probably a major role in the lignin degradation of wood, but little detail is known about such fungal enzymatic activities on lignin (further reading in Chapter 17 of this book).…”

Section: Heterobasidion Infested Wood -Naturally Activated Fibres As mentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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Function of laccase in the white-rot fungus Fomes annosus

Cited by 67 publications

References 13 publications

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Degradation of lignocellulosic residues by polyporus versicolor and the effect of moisture contents and phenolic compounds

Wood production, wood technology, and biotechnological impacts

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Function of laccase in the white-rot fungus Fomes annosus

Cited by 67 publications

References 13 publications

Efficient production of Al(OH)3-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Efficient production of Al(OH)3-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Degradation of lignocellulosic residues by polyporus versicolor and the effect of moisture contents and phenolic compounds

Wood production, wood technology, and biotechnological impacts

Contact Info

Product

Resources

About

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening

Efficient production of Al(OH)₃-immobilized laccase with aHeterobasidion annosumstrain selected by microplate screening