Function and Compensatory Mechanisms Among the Components of the Chloroplastic Redox Network

Souza, Paulo V L; Lima‐Melo, Yugo; Carvalho, Fernanda Torres de; Reichheld, Jean‐Philippe; Fernie, Alisdair R.; Silveira, Joaquim Albenísio Gomes da; Daloso, Danilo de Menezes

doi:10.1080/07352689.2018.1528409

Cited by 15 publications

(14 citation statements)

References 209 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The precise elucidation of the function of specific TRX isoforms has been a great challenge in plant biology, mostly due to the high number of TRX isoforms and the presence of other compensatory redox mechanisms found in plants (Geigenberger et al, ; Souza et al, ). Nevertheless, trxh mutants have been usually characterized by common functions in seed germination of cereals.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Fonseca‐Pereira

Souza

Hou

et al. 2019

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Thioredoxins (TRXs) are important proteins involved in redox regulation of metabolism. In plants, it has been shown that the mitochondrial metabolism is regulated by the mitochondrial TRX system. However, the functional significance of TRX h2, which is found at both cytosol and mitochondria, remains unclear. Arabidopsis plants lacking TRX h2 showed delayed seed germination and reduced respiration alongside impaired stomatal and mesophyll conductance, without impacting photosynthesis under ambient O2 conditions. However, an increase in the stoichiometry of photorespiratory CO2 release was found during O2‐dependent gas exchange measurements in trxh2 mutants. Metabolite profiling of trxh2 leaves revealed alterations in key metabolites of photorespiration and in several metabolites involved in respiration and amino acid metabolism. Decreased abundance of serine hydroxymethyltransferase and glycine decarboxylase (GDC) H and L subunits as well as reduced NADH/NAD+ ratios were also observed in trxh2 mutants. We further demonstrated that the redox status of GDC‐L is altered in trxh2 mutants in vivo and that recombinant TRX h2 can deactivate GDC‐L in vitro, indicating that this protein is redox regulated by the TRX system. Collectively, our results demonstrate that TRX h2 plays an important role in the redox regulation of mitochondrial photorespiratory metabolism.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Lack Of Trx H2 Delays Seed Germinationmentioning

confidence: 99%

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Fonseca‐Pereira

Souza

Hou

et al. 2019

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, ROS play an important role in signaling pathways essential for acclimation to environmental conditions (for recent reviews, see Mittler, 2017; Czarnocka and Karpiński, 2018; Mullineaux et al, 2018). ROS can induce signaling responses directly, or indirectly by driving redox changes that modulate signaling networks (de Souza et al, 2017; Exposito-Rodriguez et al, 2017; Noctor et al, 2018; Souza et al, 2018). In addition, oxidation by-products, including oxidized lipids and pigments, transduce signals (Mueller et al, 2008; Mosblech et al, 2009; López et al, 2011; Ramel et al, 2012; Satoh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana

et al. 2019

Self Cite

View full text Add to dashboard Cite

Photosynthesis involves the conversion of sunlight energy into stored chemical energy, which is achieved through electron transport along a series of redox reactions. Excess photosynthetic electron transport might be dangerous due to the risk of molecular oxygen reduction, generating reactive oxygen species (ROS) over-accumulation. Avoiding excess ROS production requires the rate of electron transport to be coordinated with the capacity of electron acceptors in the chloroplast stroma. Imbalance between the donor and acceptor sides of photosystem I (PSI) can lead to inactivation, which is called PSI photoinhibition. We used a light-inducible PSI photoinhibition system in Arabidopsis thaliana to resolve the time dynamics of inhibition and to investigate its impact on ROS production and turnover. The oxidation state of the PSI reaction center and rates of CO 2 fixation both indicated strong and rapid PSI photoinhibition upon donor side/acceptor side imbalance, while the rate of inhibition eased during prolonged imbalance. PSI photoinhibition was not associated with any major changes in ROS accumulation or antioxidant activity; however, a lower level of lipid oxidation correlated with lower abundance of chloroplast lipoxygenase in PSI-inhibited leaves. The results of this study suggest that rapid activation of PSI photoinhibition under severe photosynthetic imbalance protects the chloroplast from over-reduction and excess ROS formation.

show abstract

“…Recently, Souza et al (2018) highlighted the complexity of plant redox system which is composed of numerous players that act together to adjust redox metabolism and may be able to compensate one another in order to maintain homeostasis. They proposed that redundancy and complementarity between different components of the redox network enable plants to grow efficiently under both normal and stressed conditions [ 55 ]. In this work, in addition to Catalase2, among the upregulated transcripts in Snakin-1 silenced line we also identified genes involved in cell redox homeostasis such as: Nucleoredoxin, Peroxidases and Thioredoxins ( Fig 4 , S2 Table ).…”

Section: Discussionmentioning

confidence: 99%

Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

et al. 2019

View full text Add to dashboard Cite

Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that Snakin-1 is involved in plant defense to pathogens as well as in plant growth and development, but its mechanism of action has not been completely elucidated yet. Here, we showed that leaves of Snakin-1 silenced potato transgenic plants exhibited increased levels of reactive oxygen species and significantly reduced content of ascorbic acid. Furthermore, Snakin-1 silencing enhanced salicylic acid content in accordance with an increased expression of SA-inducible PRs genes. Interestingly, gibberellic acid levels were also enhanced and transcriptome analysis revealed that a large number of genes related to sterol biosynthesis were downregulated in these silenced lines. Moreover, we demonstrated that Snakin-1 directly interacts with StDIM/DWF1, an enzyme involved in plant sterols biosynthesis. Additionally, the analysis of the expression pattern of PStSN1::GUS in potato showed that Snakin-1 is present mainly in young tissues associated with active growth and cell division zones. Our comprehensive analysis of Snakin-1 silenced lines demonstrated for the first time in potato that Snakin-1 plays a role in redox balance and participates in a complex crosstalk among different hormones.

show abstract

Function and Compensatory Mechanisms Among the Components of the Chloroplastic Redox Network

Cited by 15 publications

References 209 publications

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism

Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana

Snakin-1 affects reactive oxygen species and ascorbic acid levels and hormone balance in potato

Contact Info

Product

Resources

About