Fumonisins in Maize: <i>Can We Reduce Their Occurrence?</i>

Munkvold, Gary P.; Desjardins, Anne E.

doi:10.1094/pdis.1997.81.6.556

Cited by 433 publications

(357 citation statements)

References 52 publications

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“…A. flavus can infect maize pre-and post-harvest and an increase in aflatoxin content can occur if the phases of drying and storage are poorly managed [29]. Several phytopathogenic species of Fusarium are found to be associated with maize including F. verticillioides, F. proliferatum, F. graminearum and F. anthophilum [30,31].…”

Section: Discussionmentioning

confidence: 99%

Detection of Fungi Infecting Maize (Zea mays L.) Seeds in Different Storages Around Jimma, Southwestern Ethiopia

Tsedaley¹,

Adugna²

2016

J Plant Pathol Microbiol

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Maize is attacked by more than sixty diseases and a number of species of insect pests in the field as well as in the storage. Fungi are among the principal causes of deterioration and yield loss on farmers' maize during the storage period. Among the storage fungal pathogens Aspergillus, Fusarium and Penicillium are the most predominant species attacking maize seed and resulting in reduction in seed germination. The study was conducted at the Jimma University College of Agriculture and Veterinary Medicine in plant pathology laboratory. Three maize varieties and two levels of disinfection were used and arranged in complete block design with five replications. The highest frequency of Aspregilus spp. (40.4%) at farmer preserved seed with surface disinfected kernels on agar plate were recorded. The highest relative density of Fusarium spp. (51%) was only recorded on agar plate test from the farmer preserved seed without. The lowest germination percentage (62%) were recorded on the farmer preserved seed which was not disinfected. The Aspregilus spp. are the most dominant fungi followed by Fusarium spp. isolated in this study as well as in Ethiopia. These fungi are important in producing secondary metabolites which are carcinogenic to both humans and animals.

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Section: Discussionmentioning

confidence: 99%

Detection of Fungi Infecting Maize (Zea mays L.) Seeds in Different Storages Around Jimma, Southwestern Ethiopia

Tsedaley¹,

Adugna²

2016

J Plant Pathol Microbiol

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“…We next investigated the accumulation of dolabralexins in the response of maize to elicitation with Fusarium verticillioides and Fusarium graminearum, which are causal agents of seedling blights, stalk rots, ear rots, and mycotoxin contamination (Munkvold, 1997;Goswami and Kistler, 2004;Baldwin et al, 2014). For this purpose, roots of 53-d-old Mo17 plants were inoculated with live F. verticillioides or F. graminearum spores and harvested for the analysis of both metabolites and transcripts 7 d later.…”

Section: Dolabralexins Are Induced In Biotic and Abiotic Stress Respomentioning

confidence: 99%

Discovery, Biosynthesis and Stress-Related Accumulation of Dolabradiene-Derived Defenses in Maize

et al. 2018

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“…It has caused stalk, root and ear rot, resulting in severe economic losses in corn, other cereals and food crops (Nelson 1992). F. verticillioides infection in corn causes potential contamination by fumonisins and other mycotoxins (Munkvold & Desjardins 1997), which is further complicated due to the high frequency of fumonisins in kernels that show no symptoms (Nelson et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Development of polyclonal antibodies againstFusarium verticillioidesexoantigens

Biazon

Meirelles

Ono

et al. 2006

Food and Agricultural Immunology

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Exoantigens from Fusarium verticillioides strains were analysed by polyacrylamide gel electrophoresis (SDS-PAGE), enzyme-linked immunosorbent assay (ELISA) and Western blot and were used for production of polyclonal antibodies. Rabbits were immunized with exoantigens from strains 97K, 162A and 113F. Polyclonal antibodies against F. verticillioides 97K exoantigen showed the highest titre in ELISA (1:12.800) and were used in further studies. The crossreactivity of anti-97K antibodies with 14 species from 7 other fungal genera was low, ranging from 4.5 Á/14.7%; the highest reactivities (51.2 Á/76.0%) were observed in Fusarium genera. The detection limit of exoantigen by indirect competitive ELISA was 0.27 mg/ml. Western blot analysis showed two immunodominant antigens (67 kDa and 113 kDa) common to all the F. verticillioides strains analysed. The results suggest that polyclonal antibodies raised against exoantigens from F. verticillioides 97K are promising for Fusarium sp. immunodetection.

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Fumonisins in Maize: Can We Reduce Their Occurrence?

Cited by 433 publications

References 52 publications

Detection of Fungi Infecting Maize (Zea mays L.) Seeds in Different Storages Around Jimma, Southwestern Ethiopia

Detection of Fungi Infecting Maize (Zea mays L.) Seeds in Different Storages Around Jimma, Southwestern Ethiopia

Discovery, Biosynthesis and Stress-Related Accumulation of Dolabradiene-Derived Defenses in Maize

Development of polyclonal antibodies againstFusarium verticillioidesexoantigens

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