Fumonisin B1, zearalenone and deoxynivalenol production by Fusarium moniliforme, F proliferatum and F graminearum in mixed cultures on irradiated maize kernels

Velluti, Andrea; Marı́n, Sonia; Gonzalez, Roger V.; Ramos, Ana M. González; Sanchis, Vicente

doi:10.1002/1097-0010(20010101)81:1<88::aid-jsfa787>3.3.co;2-h

Cited by 11 publications

(16 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast to our observations, stimulation of F. graminearum DON production was reported in the competition with Fusarium verticillium or Fusarium proliferatum on irradiated maize kernels (Velluti et al , 2001). Fungal biomasses were not quantified in this study, and it is possible that higher DON levels resulted from a stimulated F. graminearum growth.…”

Section: Resultscontrasting

confidence: 99%

“…We used our PCR assays to test the competitive ability of a DON-producing F. graminearum and its nontoxigenic mutant against the biocontrol candidate T. atroviride in a standardized microcosm with autoclaved maize leaf tissue. As we found no evidence for a recently suggested competitive advantage conferred by F. graminearum DON production (Velluti et al, 2001;Lutz et al, 2003), biocontrol application of Trichoderma is unlikely to increase selection for toxigenic F. graminearum specimens; this needs to be proven under field conditions. In our competition assays, we further observed partially reduced DON production per F. graminearum wild-type biomass in the presence of T. atroviride.…”

Section: Role Of Fusarium Graminearum Don Production In the Interacticontrasting

confidence: 79%

See 1 more Smart Citation

A microsatellite based method for quantification of fungi in decomposing plant material elucidates the role of Fusarium graminearum DON production in the saprophytic competition with Trichoderma atroviride in maize tissue microcosms

Naef

Senatore

Défago³

2006

FEMS Microbiology Ecology

View full text Add to dashboard Cite

Common PCR assays for quantification of fungi in living plants cannot be used to study saprophytic colonization of fungi because plant decomposition releases PCR-inhibiting substances and saprophytes degrade the plant DNA which could serve as internal standard. The microsatellite PCR assays presented here overcome these problems by spiking samples prior to DNA extraction with mycelium of a reference strain. PCR with fluorescent primers co-amplifies microsatellite fragments of different length from target and reference strains. These fragments were separated in a capillary sequencer with fluorescence detection. The target/reference ratio of fluorescence signal was used to calculate target biomass in the sample. Such PCR assays were developed for the mycotoxin deoxynivalenol (DON)-producing wheat and maize pathogen Fusarium graminearum and the biocontrol agent Trichoderma atroviride, using new microsatellite markers. In contrast to real-time PCR assays, the novel PCR assays showed reliable fungal biomass quantification in samples with differentially decomposed plant tissue. The PCR assays were used to quantify the two fungi after competitive colonization of autoclaved maize leaf tissue in microcosms. Using a DON-producing F. graminearum wild-type strain and its nontoxigenic mutant we found no evidence for a role of DON production in F. graminearum defense against T. atroviride. The presence of T. atroviride resulted in a 36% lower wild-type DON production per biomass.

show abstract

Section: Resultscontrasting

confidence: 99%

Section: Role Of Fusarium Graminearum Don Production In the Interacticontrasting

confidence: 79%

A microsatellite based method for quantification of fungi in decomposing plant material elucidates the role of Fusarium graminearum DON production in the saprophytic competition with Trichoderma atroviride in maize tissue microcosms

Naef

Senatore

Défago³

2006

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…(2000a) noted that the growth rate of F. graminearum was relatively unaffected by the presence of competing species, whereas the growth of F. proliferatum and F. moniliforme was reduced. However, a similar study by the same authors (Velluti et al ., 2000b) found that mixed culture of F. graminearum with either F. proliferatum or F. moniliforme led to a larger total fungal population than occurred with the growth of F. graminearum alone. Their study quantified the total population by colony counts only, without quantifying each species individually.…”

Section: Discussionmentioning

confidence: 73%

“…Previous studies have indicated that the growth and toxin production of toxigenic Fusarium species can be affected by the presence of competing fungi (Ramakrishna et al ., 1996; Velluti et al ., 2000a) and that toxin production can, in some circumstances, be stimulated in these interactions. Much of the work in this area has relied on the isolation and enumeration of colony‐forming units to quantify the mixed population (Ramakrishna et al ., 1996), sometimes without specific quantification of each pathogen (Velluti et al ., 2000a,b). Molecular assays to detect and individually quantify the major pathogens involved in the Fusarium ear blight and stem base disease complexes have now been developed (Nicholson et al ., 1996; 1998), and these assays are used here to investigate the nature of interactions between some components of the disease complexes.…”

Section: Introductionmentioning

confidence: 99%

Competitive interactions between Microdochium nivale var. majus, M. nivale var. nivale and Fusarium culmorum in planta and in vitro

Simpson¹,

Thomsett²,

Nicholson³

2003

Environmental Microbiology

View full text Add to dashboard Cite

Microdochium nivale var. majus and var. nivale are economically important fungal pathogens of cereal seedlings, stem bases and ears, as is the toxigenic species Fusarium culmorum. Competition experiments on seedlings support an earlier report of differential host preference between the varieties of M. nivale on wheat and rye seedlings at 15 degrees C, but showed that it does not extend across a broad range of temperatures. The studies showed that, although interaction is disadvantageous to the less virulent pathogen, it does not confer an advantage to the more virulent pathogen. In mixed inoculum experiments on wheat seedlings at 15 degrees C and 20 degrees C, F. culmorum suppressed the growth of both varieties of M. nivale. However, if M. nivale var. majus became established on the seedlings, it was able to co-suppress colonization of wheat seedlings by F. culmorum. In contrast M. nivale var. nivale did not suppress F. culmorum significantly. The growth of M. nivale var. majus and F. culmorum was also co-suppressed in liquid culture. Significantly, the accumulation of deoxynivalenol mycotoxin was also reduced in the mixed in vitro culture compared with axenic culture of F. culmorum. However, in vitro interaction studies on solidified media were of only limited use in predicting the outcome of competitions in planta.

show abstract

“…, 1999) in maize ears whereas in vitro studies yielded opposite results (Marín et al. , 1998; Velluti et al. , 2000, 2001).…”

Section: Introductionmentioning

confidence: 94%

Interactions between Fusarium verticillioides and Fusarium graminearum in maize ears and consequences for fungal development and mycotoxin accumulation

et al. 2011

View full text Add to dashboard Cite

Fungal interactions of Fusarium verticillioides and F. graminearum in maize ears and the impact on fungal development and toxin accumulation were investigated in a 2-year field study at two locations in France. Maize ears were inoculated either with a spore mixture of F. graminearum and F. verticillioides or using a sequential inoculation procedure consisting of a first inoculation with F. graminearum followed by a second with F. verticillioides 1 week later. Toxin and fungal biomass were assessed on mature kernels, using HPLC and quantitative PCR. Correlation between the levels of DNA and toxin was high concerning F. graminearum DNA and deoxynivalenol (R 2 = 0AE73) and moderate for F. verticillioides DNA and fumonisin (R 2 = 0AE44). Fusarium graminearum DNA either decreased in mixed inoculations or was not influenced by subsequent inoculations with F. verticillioides, compared to single inoculations. In contrast, F. verticillioides DNA either significantly increased or was not affected in mixed and sequential inoculations. In two of the replicates, it can be assumed that natural contamination by F. verticillioides was favoured by previous contamination with F. graminearum. Overall, the results suggest that F. verticillioides has competitive advantages over the F. graminearum strains. Additionally, the data provide, for the first time, key evidence that previous contamination by F. graminearum in maize ears can facilitate subsequent infections by F. verticillioides.

show abstract

Fumonisin B1, zearalenone and deoxynivalenol production by Fusarium moniliforme, F proliferatum and F graminearum in mixed cultures on irradiated maize kernels

Cited by 11 publications

References 11 publications

A microsatellite based method for quantification of fungi in decomposing plant material elucidates the role of Fusarium graminearum DON production in the saprophytic competition with Trichoderma atroviride in maize tissue microcosms

A microsatellite based method for quantification of fungi in decomposing plant material elucidates the role of Fusarium graminearum DON production in the saprophytic competition with Trichoderma atroviride in maize tissue microcosms

Competitive interactions between Microdochium nivale var. majus, M. nivale var. nivale and Fusarium culmorum in planta and in vitro

Interactions between Fusarium verticillioides and Fusarium graminearum in maize ears and consequences for fungal development and mycotoxin accumulation

Contact Info

Product

Resources

About